Measurement of Glucocorticoid Metabolite Concentrations in Faeces of Domestic Livestock

Möstl, Erich; Messmann, S.; Bagu, E; Robia, C.; Palme, Rüpert

doi:10.1046/j.1439-0442.1999.00256.x

Cited by 167 publications

(24 citation statements)

References 13 publications

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“…HPLC analysis and generation of immunoreactivity profiles of samples at time 0 and at different times of storage are needed, however, to substantiate this idea ( cf. Möstl et al 1999). …”

Section: Discussionmentioning

confidence: 99%

Methodological Considerations in the Analysis of Fecal Glucocorticoid Metabolites in Tufted Capuchins (Cebus apella)

et al. 2013

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Analysis of fecal glucocorticoid (GC) metabolites has recently become the standard method to monitor adrenocortical activity in primates noninvasively. However, given variation in the production, metabolism, and excretion of GCs across species and even between sexes, there are no standard methods that are universally applicable. In particular, it is important to validate assays intended to measure GC production, test extraction and storage procedures, and consider the time course of GC metabolite excretion relative to the production and circulation of the native hormones. This study examines these four methodological aspects of fecal GC metabolite analysis in tufted capuchins (Cebus apella). Specifically, we conducted an adrenocorticotrophic hormone (ACTH) challenge on one male and one female capuchin to test the validity of four GC enzyme immunoassays (EIAs) and document the time course characterizing GC metabolite excretion in this species. In addition, we compare a common field-friendly technique for extracting fecal GC metabolites to an established laboratory extraction methodology and test for effects of storing “field extracts” for up to 1 yr. Results suggest that a corticosterone EIA is most sensitive to changes in GC production, provides reliable measures when extracted according to the field method, and measures GC metabolites which remain highly stable after even 12 mo of storage. Further, the time course of GC metabolite excretion is shorter than that described yet for any primate taxa. These results provide guidelines for studies of GCs in tufted capuchins, and underscore the importance of validating methods for fecal hormone analysis for each species of interest.

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“…HPLC analysis and generation of immunoreactivity profiles of samples at time 0 and at different times of storage are needed, however, to substantiate this idea ( cf. Möstl et al 1999). …”

Section: Discussionmentioning

confidence: 99%

Methodological Considerations in the Analysis of Fecal Glucocorticoid Metabolites in Tufted Capuchins (Cebus apella)

et al. 2013

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“…Afterwards the ether phase was decanted and dried down. The extract was re-dissolved in assay buffer and the concentration of 11,17-dioxodandrostanes (11,17-DOA), a group of cortisol metabolites, was measured with an 11-oxoaetiocholanolone enzyme immunoassay (EIA), previously described [55] and successfully validated for use in horses [56]. …”

Section: Methodsmentioning

confidence: 99%

Plasma cortisol and faecal cortisol metabolites concentrations in stereotypic and non-stereotypic horses: do stereotypic horses cope better with poor environmental conditions?

et al. 2013

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BackgroundStereotypic behaviours, i.e. repetitive behaviours induced by frustration, repeated attempts to cope and/or brain dysfunction, are intriguing as they occur in a variety of domestic and captive species without any clear adaptive function. Among the different hypotheses, the coping hypothesis predicts that stereotypic behaviours provide a way for animals in unfavourable environmental conditions to adjust. As such, they are expected to have a lower physiological stress level (glucocorticoids) than non-stereotypic animals. Attempts to link stereotypic behaviours with glucocorticoids however have yielded contradictory results. Here we investigated correlates of oral and motor stereotypic behaviours and glucocorticoid levels in two large samples of domestic horses (NStudy1 = 55, NStudy2 = 58), kept in sub-optimal conditions (e.g. confinement, social isolation), and already known to experience poor welfare states. Each horse was observed in its box using focal sampling (study 1) and instantaneous scan sampling (study 2). Plasma samples (collected in study 1) but also non-invasive faecal samples (collected in both studies) were retrieved in order to assess cortisol levels.ResultsResults showed that 1) plasma cortisol and faecal cortisol metabolites concentrations did not differ between horses displaying stereotypic behaviours and non-stereotypic horses and 2) both oral and motor stereotypic behaviour levels did not predict plasma cortisol or faecal cortisol metabolites concentrations.ConclusionsCortisol measures, collected in two large samples of horses using both plasma sampling as well as faecal sampling (the latter method minimizing bias due to a non-invasive sampling procedure), therefore do not indicate that stereotypic horses cope better, at least in terms of adrenocortical activity.

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“…Asimismo, las muestras deben ser congeladas de inmediato a -20 °C para prevenir la degradación de los esteroides por la actividad enzimática de las bacterias presentes en las heces (Bokkenheuser et al, 1978;Möstl et al, 1999;Arias et al, 2013). Figura 1.…”

Section: Metabolitos De Cortisol En Hecesunclassified

Cortisol como Indicador Fiable del Estrés en Alpacas y Llamas

Arias

Velapatiño

2015

Rev. investig. vet. Perú

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RESUMENSe revisan investigaciones sobre medición de cortisol y sus metabolitos en alpacas y llamas. Las concentraciones de cortisol en muestras de plasma, suero y saliva, así como de metabolitos de cortisol en muestras de heces, se elevan luego de situaciones de estrés. La interpretación de estos resultados debe complementarse con otros signos fisiológicos y observaciones del comportamiento.Palabras clave: bienestar animal; camélidos; comportamiento; cortisol; estrés ABSTRACTResearch papers on the measurement of cortisol and its metabolites in alpacas and llamas were reviewed. Plasma, serum, saliva cortisol and fecal cortisol metabolites concentrations increase after stress events. The interpretation of these results should be complemented with additional physiological signs and behavioral observations.

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Measurement of Glucocorticoid Metabolite Concentrations in Faeces of Domestic Livestock

Cited by 167 publications

References 13 publications

Methodological Considerations in the Analysis of Fecal Glucocorticoid Metabolites in Tufted Capuchins (Cebus apella)

Methodological Considerations in the Analysis of Fecal Glucocorticoid Metabolites in Tufted Capuchins (Cebus apella)

Plasma cortisol and faecal cortisol metabolites concentrations in stereotypic and non-stereotypic horses: do stereotypic horses cope better with poor environmental conditions?

Cortisol como Indicador Fiable del Estrés en Alpacas y Llamas

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