Measurement of Henry's law constants for C1 and C2 chlorinated hydrocarbons

Gossett, James M.

doi:10.1021/es00156a012

Cited by 770 publications

(542 citation statements)

References 2 publications

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“…This nomenclature allowed mass balances to be expressed more easily (Egli et al 1987). Our calculations with the Henry's constant (Gossett 1987) indicated that t> 97.5% of DCM was in the aqueous phase.…”

Section: Methodsmentioning

confidence: 86%

Dichloromethane utilized by an anaerobic mixed culture: acetogenesis and methanogenesis

Stromeyer¹,

Winkelbauer²,

Kohler³

et al. 1991

Biodegradation

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Dichloromethane (8.9 mg/1) was eliminated from industrially polluted, anaerobic groundwater in a fixed-bed reactor (43 m 3) which was packed with activated charcoal and operated continuously for over three years. The elimination of dichloromethane over this period was some ten-fold in excess of the sorptive capacity of the charcoal, and the elimination (3.7 mg/h. [kg of charcoal]: residence time, 49 h) was tentatively attributed to deha]ogenative microorganisms immobilized on the charcoal. Anaerobic enrichment cultures, with dichloromethane as the sole added source of carbon and energy, were inoculated with material from the reactor. Reproducibly complete substrate disappearance in subcultures was observed when traces of groundwater (1%) or yeast extract (0.01%) were supplied. Fed-batch experiments under an atmosphere of CO2 plus N2 led to the conversion in 11 days of 11 mM dichloromethane to 3 mM acetate and 2 mM methane, with a growth yield of 0.4 g of protein/mol of dichloromethane; insignificant amounts (< 1/zM) of chloromethane accumulated. Methanogenesis could be inhibited by 50 mM 2-bromoethane sulfonate without any effect on the dehalogenation rate. The maximum dehalogenation rate was 0.13 mmol dichloromethane/h-1 (2.6 mkat/ kg of protein).

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Section: Methodsmentioning

confidence: 86%

Dichloromethane utilized by an anaerobic mixed culture: acetogenesis and methanogenesis

Stromeyer¹,

Winkelbauer²,

Kohler³

et al. 1991

Biodegradation

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“…Assays of enzymatic activity were done at 30°C in a 3-ml volume in 12.4-ml serum flasks with gas-tight rubber stoppers. The H 4 folate-dependent dehalogenation of chloromethane was measured by following the consumption of chloromethane by gas chromatography (20) by using a Henry constant of 0.43, calculated by interpolation to 30°C of the published value for chloromethane (21). Dehalogenation was also determined by monitoring the chloromethane-dependent formation of CH 3 -H 4 folate from H 4 folate.…”

Section: Methodsmentioning

confidence: 99%

A corrinoid-dependent catabolic pathway for growth of aMethylobacteriumstrain with chloromethane

Vannelli

Meßmer

Studer

et al. 1999

Proc. Natl. Acad. Sci. U.S.A.

103

165

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Methylobacterium sp. strain CM4, an aerobic methylotrophic ␣-proteobacterium, is able to grow with chloromethane as a carbon and energy source. Mutants of this strain that still grew with methanol, methylamine, or formate, but were unable to grow with chloromethane, were previously obtained by miniTn5 mutagenesis. The transposon insertion sites in six of these mutants mapped to two distinct DNA fragments. The sequences of these fragments, which extended over more than 17 kb, were determined. Sequence analysis, mutant properties, and measurements of enzyme activity in cell-free extracts allowed the definition of a multistep pathway for the conversion of chloromethane to formate. The methyl group of chloromethane is first transferred by the protein CmuA (cmu: chloromethane utilization) to a corrinoid protein, from where it is transferred to H 4 folate by CmuB. Both CmuA and CmuB display sequence similarity to methyltransferases of methanogenic archaea. In its C-terminal part, CmuA is also very similar to corrinoid-binding proteins, indicating that it is a bifunctional protein consisting of two domains that are expressed as separate polypeptides in methyl transfer systems of methanogens. The methyl group derived from chloromethane is then processed by means of pterinelinked intermediates to formate by a pathway that appears to be distinct from those already described in Methylobacterium. Remarkable features of this pathway for the catabolism of chloromethane thus include the involvement of a corrinoiddependent methyltransferase system for dehalogenation in an aerobe and a set of enzymes specifically involved in funneling the C1 moiety derived from chloromethane into central metabolism.

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“…The column temperature was 160~ which resulted in VC retention times of 0.65 rain. The total amount of VC in the sample was determined using Henry's constants published by Gossett (1987).…”

Section: Analyticalmentioning

confidence: 99%

Vinyl Chloride Biodegradation with Methanotrophic Attached Films

Nelson

Jewell

1993

J. Environ. Eng.

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ABSTRACT; Methanotrophic degradation of vinyl chloride (VC) is investigated using a laboratory-scale methanotrophic attached-film expanded-bed (MAFEB) bioreactor. This study provides a basis for applying a microbial cometabolizing reaction to practical treatment of toxic chlorinated compounds. The MAFEB reactor was operated at 20~ with in fluent VC concentrations ranging from 1,800 to 9,600 I~g/L and bed hydraulic retention times ranging from 3.7 to 7.6 h. VC effluent concentrations during steady continuous operation ranged from 3 to 140 ixg/L, with most values less than 26 p,g/L, resulting in removal efficiencies of 96.3% to 99.8%. The maximum continuous-flow VC degradation rate observed at 20~ was 2.

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Measurement of Henry's law constants for C1 and C2 chlorinated hydrocarbons

Cited by 770 publications

References 2 publications

Dichloromethane utilized by an anaerobic mixed culture: acetogenesis and methanogenesis

Dichloromethane utilized by an anaerobic mixed culture: acetogenesis and methanogenesis

A corrinoid-dependent catabolic pathway for growth of aMethylobacteriumstrain with chloromethane

Vinyl Chloride Biodegradation with Methanotrophic Attached Films

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