Measurement of Histamine‐Releasing Activity

Mongar, J. L.

doi:10.1002/9780470718957.ch12

Cited by 12 publications

(2 citation statements)

References 30 publications

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“…Histamine release in man and in laboratory animals can be determined by various in-vivo and in-vitro methods [8,41,42,43]. Most frequently the following parameters were measured : (a) Increase of concentration and output of histamine in several body fluids, especially in blood and urine.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of histamine elimination curves in plasma and whole blood of several circulatory regions: A method for studying kinetics of histamine release in the whole animal

et al. 1974

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Histamine concentrations in canine whole blood and plasma were determined under several pharmacological, pathophysiological, and clinical conditions using fluorometric methods.The specificity of the assay for whole-blood histamine was investigated by comparing 3 purification procedures for the isolation of histamine from whole blood including butanol extraction (Shore), ion-exchange chromatography on Dowex 50 W-X 8, and the combination of these 2 methods (Lorenz). Histamine in whole blood was identified in analytical and preparative samples by fluorescence spectra, thin-layer chromatography, degradation by diamine oxidase from pig kidney and inactivation by histamine methyltransferase from guinea-pig brain as well as by biological tests on the isolated guinea-pig ileum. Since butanol extraction resulted in significantly higher 'histamine' values than the other two purification procedures, ionexchange chromatography on Dowex 50 was recommended as the method of choice for the specific determination of histamine in doffs whole blood.Normal values of histamine concentrations in canine plasma were tentatively estimated. They depended on the time between pretreatment of the animals (anaesthesia, operation) and the collection of blood and showed an approximately logarithmic normal distribution. The median, the lower/upper quartiles and the range of the plasma histamine levels obtained 30 minutes after the end of pretreatment were 0.2, 0-0.4 and 0-1.2 ng/ml, respectively. Nearly 50 % of the values were zero (below 0.1 ng according to the sensitivity of the method), only 1 ~ of them exceeded slightly 1 ng/ml. Thus histamine release by drugs or by other medical treatments was only stated, when plasma histamine levels exceeded 1 ng/ml and decreased in a way to give an elimination carve of approximately first-order kinetics (Bateman function).Histamine concentrations in dog's whole blood showed approximately a logarithmic normal distribution. The median, lower/upper quartiles and range were 47, 34/75 and 13-209 ng/ml respectively.

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Section: Discussionmentioning

confidence: 99%

Evaluation of histamine elimination curves in plasma and whole blood of several circulatory regions: A method for studying kinetics of histamine release in the whole animal

et al. 1974

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“…Although this drug is active as histamine releaser in mice, isolated mouse peritoneal mast cells are not responsive [7]. Tissue variations in the action of compound 48/80 are well known since the early experiments with this drug [8]. To study the influence of lysophosphatidylserine on mast cells of different origin a comparison has been made of the activity of this compound on preparations obtained from various rodents.…”

Section: Introductionmentioning

confidence: 99%

Different responses of rodent mast cells to lysophosphatidylserine

et al. 1984

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The lysophosphatidylserine-induced activation of mast cells has been studied in preparations obtained from different rodents. In mouse and gerbil peritoneal mast cells lysophosphatidylserine behaves as an agonist, inducing noncytotoxic histamine release at 0.2-8 microM. In rat peritoneal and pleural mast cells lysophosphatidylserine is ineffective, but the histamine-releasing activity becomes manifest upon the addition of suboptimal concentrations of other mast cell activators. The common structure-activity relationship shows the link between these effects of lysophosphatidylserine but the calcium requirement indicates differences in the mechanism of action. Histamine release in mouse mast cells is independent of external calcium. Thus, lysophosphatidylserine induces mobilization of endogenous calcium stores in these cells. By contrast, histamine release in gerbil and rat mast cells is dependent on the addition of external calcium indicating that the phospholipid promotes calcium influx. While in gerbil mast cells calcium influx is promoted by lysophosphatidylserine alone, in rat it requires the combined action of the phospholipid and other mast cell agonists. Differently from lysophosphatidylserine, compound 48/80 elicits histamine release in rat and gerbil mast cells. Mouse mast cells are unaffected. Thus, gerbil mast cells are the only preparation in which the action of these two agonists can be observed simultaneously.

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Histamine Release by Basic Compounds

Rothschild¹

1966

Histamine and Anti-Histaminics

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Measurement of Histamine‐Releasing Activity

Cited by 12 publications

References 30 publications

Evaluation of histamine elimination curves in plasma and whole blood of several circulatory regions: A method for studying kinetics of histamine release in the whole animal

Evaluation of histamine elimination curves in plasma and whole blood of several circulatory regions: A method for studying kinetics of histamine release in the whole animal

Different responses of rodent mast cells to lysophosphatidylserine

Histamine Release by Basic Compounds

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