Measurement of IgG Antibody and Airborne Antigen to Control an Industrial Outbreak of Hypersensitivity Pneumonitis

Reed, Charles E.; Swanson, Mark C.; Lopez, Mae K.; Ford, Alex; Major, J; Witmer, W B; Valdes, T B

doi:10.1097/00043764-198303000-00014

Cited by 47 publications

(21 citation statements)

References 5 publications

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“…In previous studies similar symptoms have been described in relation with endotoxin fever, humidifier fever, or Monday morning fever and extrinsic allergic alveolitis (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13). As etiologic agent(s) fungi, bacteria, amoeba, endotoxins, and antigenic material from humidifiers have been suggested.…”

mentioning

confidence: 52%

Relationship of airborne microorganisms with the lung function and leucocyte levels of workers with a history of humidifier fever.

Kateman¹,

Heederik²,

Pal³

et al. 1990

Scand J Work Environ Health

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mentioning

confidence: 52%

Relationship of airborne microorganisms with the lung function and leucocyte levels of workers with a history of humidifier fever.

Kateman¹,

Heederik²,

Pal³

et al. 1990

Scand J Work Environ Health

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“…Several studies [10][11][12][13] as well as our own investigations including inhalative challenge tests clearly demonstrate the causative role of microbially contaminated water in humidifier lung disease. Approximately two thirds of the workers of the printing company were found to be immunologically sensitized to antigens in the water supply and 2-26% of them had IgG antibodies to extracts of the mi croorganisms tested.…”

Section: Discussionmentioning

confidence: 86%

Increased Prevalence of IgG-lnduced Sensitization and Hypersensitivity Pneumonitis (Humidifier Lung) in Nonsmokers Exposed to Aerosols of a Contaminated Air Conditioner

Baur¹,

Richter

Pethran

et al. 1992

Respiration

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Specific IgG antibodies against antigens of a contaminated air conditioner were estimated in serum of 134 workers of a printing company. Altogether 64% of the workers investigated revealed significantly elevated levels ( > 3 U/ml) of IgG antibodies specific to these antigens as compared to a nonexposed control group. The occurence of IgG antibodies for microbial extracts were 25% for Fusarium, 23% for Penicillium notatum, 13% for Alternaria tenuis, 12% for Aureobasidium pullulans, 9% for Sphaeropsidales species, 3% for Micropolyspora faeni, 2% for Aspergillus fumigatus and 2% for Thermoactionomyces vulgaris. Out of the 86 workers with elevated IgG antibodies for air conditioner antigens, 59 were nonsmokers. Considering a cut-off level of 10 U/ml IgG for high values, the proportion of smokers to nonsmokers becomes even more pronounced (6 to 36 respectively, binominal test p < 0.001). This is despite the fact that the distribution of smokers and nonsmokers among the 134 workers is approximately equal (60 to 74). All 3 workers with clinical diagnosis of humidifier lung or humidifier fever belonged to the nonsmoker group. Our findings indicate that crude water extracts of contaminated air conditioners are the best choice as antigen source for the diagnosis of humidifier lung in exposed workers. Nonsmokers are shown to have a high risk for immunological sensitization.

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“…The use of pooled sera of sensitized people may be practical in some special cases and environments [15,16]. A rabbit an tiserum offers, however, the advantage of availability and high titer allowing long-term studies with consis tent results.…”

Section: Discussionmentioning

confidence: 99%

Enzyme-Linked Immunosorbent Assay (ELISA) Inhibition Method to Estimate the Level of Airborne Bovine Epidermal Antigen in Cowsheds

Virtanen¹,

Louhelainen

Mäntyjärvi³

1986

Int Arch Allergy Immunol

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A method based on inhibition of the enzyme-linked immunosorbent assay was developed for measuring airborne bovine epidermal antigen (BEA) in cowsheds. BEA extract served as the solid-phase reactant competing with the sample for binding of a rabbit immune serum. A sample of swine epithelial extract was used to ascertain the specificity of the reaction. Cowshed air samples were collected according to a standardized volumetric filter method. A specific inhibiting activity was detected in all samples tested corresponding to concentrations between 137ng/m³ and 19.8 μg/m³. The intertest coefficient of variation of cowshed samples was 9.5%.

show abstract

Measurement of IgG Antibody and Airborne Antigen to Control an Industrial Outbreak of Hypersensitivity Pneumonitis

Cited by 47 publications

References 5 publications

Relationship of airborne microorganisms with the lung function and leucocyte levels of workers with a history of humidifier fever.

Relationship of airborne microorganisms with the lung function and leucocyte levels of workers with a history of humidifier fever.

Increased Prevalence of IgG-lnduced Sensitization and Hypersensitivity Pneumonitis (Humidifier Lung) in Nonsmokers Exposed to Aerosols of a Contaminated Air Conditioner

Enzyme-Linked Immunosorbent Assay (ELISA) Inhibition Method to Estimate the Level of Airborne Bovine Epidermal Antigen in Cowsheds

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