Measurement of immunoglobulin A, G, and M class rotavirus antibodies in serum and mucosal secretions

McLean, B; Sonza, Secondo; Holmes, Ian

doi:10.1128/jcm.12.3.314-319.1980

Cited by 37 publications

(24 citation statements)

References 19 publications

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“…To investigate these possibilities, we have undertaken a survey of 92 mother-infant pairs during their first 5 to 8 days postpartum in the postnatal wards of a maternity hospital. The distribution of the concentrations of rotavirusspecific immunoglobulin classes, detected by a class-specific enzyme-linked immunosorbent microassay (10,19) in the maternal lacteal secre-tions and in the maternal and cord sera, has been described previously (18). This paper relates these lacteal and serum immunoglobulin levels, neonatal fecal trypsin levels, and lacteal TI measurements to the incidence of rotavirus infection in the infants of this population.…”

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confidence: 85%

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Effects of antibodies, trypsin, and trypsin inhibitors on susceptibility of neonates to rotavirus infection

McLean¹,

Holmes²

1981

J Clin Microbiol

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Levels of antirotaviral secretory immunoglobulin A were measured by enzymelinked immunosorbent assay in colostrum and milk samples collected daily for the first 5 days postpartum from 49 mothers breast-feeding their infants. The trypsin-inhibitory capacity of these lacteal secretion samples was assessed by their ability to inhibit the hydrolysis of aN -benzoyl-DL-arginine-p-nitroaniide by trypsin. Stools passed by these breast-fed infants and by an additional 43 bottlefed infants were pooled by individual and examined by electron microscopy for rotavirus. Stool trypsin levels were estimated with the gelatin hydrolysis test. Breast-fed infants were significantly less likely to become infected with rotavirus and showed significantly lower stool tryptic activity than did bottle-fed infants. Breast-fed infants who did not excrete rotavirus over the 5-day period received milk of significantly higher antirotaviral secretory immunoglobulin A or trypsininhibitory capacity or both than breast-fed infants who were infected with rotavirus. A case of probable maternal rotavirus infection during pregnancy, producing greatly elevated lacteal antirotaviral secretory immunoglobulin A levels lasting for 2 years, was detected. Results of this study suggest that both antibodies and trypsin inhibitors in human milk can be associated with the protection of neonates against rotavirus infection in the first 5 days of life.

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confidence: 85%

“…Class-specific enzyme-linked immunosorbent microassay. Details of the method have been described previously (10,19).…”

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confidence: 99%

Effects of antibodies, trypsin, and trypsin inhibitors on susceptibility of neonates to rotavirus infection

McLean¹,

Holmes²

1981

J Clin Microbiol

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“…IgE antibodies to cows' milk were measured by the Phadebas RAST assay (Pharmacia, Uppsala, Sweden) in one batch and expressed as absolute counts per minute of isotope bound. IgG antibodies to rotavirus were measured using a modification ofan ELISA assay [14]. Based on longitudinal epidemiological data generated from this institution, a level of more than 300 rotavirus antibody units (RAU) was considered as evidence of past rotavirus infection [15].…”

Section: Total Serum Immunoglobutins and Specific Antibodiesmentioning

confidence: 99%

Possible role for rotavirus in the development of cows' milk enteropathy in infants

Firer

Hosking

Hill

1988

Clin Experimental Allergy

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In order to test the hypothesis that rotavirus infection leads to IgE hypersensitivity to cows' milk, evidence of past rotavirus infection, as reflected by the presence of antibodies, was sought in two groups of children with cows' milk allergy. The groups were defined according to the time to react to milk challenge. In group I there were fifteen children with an adverse reaction within 40 min of milk challenge. The twentyone children in group II had gastrointestinal reactions between 1 hr and 24 hr after milk challenge. The results showed a higher ineidence of antibodies to rotavirus in group II than group I (/'<0 05). Although group I patients had significantly higher levels of total and milk-specific IgE antibodies to cows' milk than those in group II, both patient groups had lower levels of serum IgG, IgA and IgM than a control population. No association between the presence of rotavirus antibodies and IgE hypersensitivity to cows' milk was found. However, an association between non-IgE milk enteropathy and previous rotavirus antibodies was demonstrated.

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“…Rotavirus-specific IgA and IgG ELISAs were performed essentially as described by McLean et al (1980) and Bishop et al (1984). Antigen was prepared by ultracentrifugation (80 000 g for 1 hour at 4°C) of clarified (5000 g for 30 minutes at 4°C) lysates ofMA 104 cell cultures and resuspended in 5 ml of 20 mM Tris-HC1 (pH 7.5).…”

Section: Isotype-specific Elisasmentioning

confidence: 99%

Homotypic and heterotypic serum and milk antibody to rotavirus in normal, infected and vaccinated horses

Browning

Chalmers

Sale

et al. 1991

Veterinary Microbiology

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The homotypic and heterotypic antibody response to rotavirus was determined in three pony mares and their foals. The normal concentrations of anti-rotavirus antibodies in mares' milk and mares' and foals' serum over the first 10 weeks post-partum were measured using IgA, IgG and rotavirus serotype-specific enzyme linked immunosorbent assays. Experimental infection of the foals with serotype 3 equine rotavirus produced a rapid, serotype-specific response which peaked 10 days after infection and a slower heterotypic response which peaked 32 days later. In contrast, vaccination of the mares with an inactivated, adjuvanted serotype 6 bovine rotavirus produced a heterotypic response similar to that of the homotypic response in both serum and milk, although the predominant response in serum was IgG, while in milk it was IgA. These results suggest that non serotype-restricted passive protection of foals against rotavirus may be achieved by parenteral vaccination of mares.

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Measurement of immunoglobulin A, G, and M class rotavirus antibodies in serum and mucosal secretions

Cited by 37 publications

References 19 publications

Effects of antibodies, trypsin, and trypsin inhibitors on susceptibility of neonates to rotavirus infection

Effects of antibodies, trypsin, and trypsin inhibitors on susceptibility of neonates to rotavirus infection

Possible role for rotavirus in the development of cows' milk enteropathy in infants

Homotypic and heterotypic serum and milk antibody to rotavirus in normal, infected and vaccinated horses

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