Measurement of ‘in situ’ mitochondrial membrane potential in Ehrlich ascites tumor cells during aerobic glycolysis

Olavarría, J S; Galindo, Clara Eugenia Maestre; Montero, Mayte; Baquero, Yolanda; Vitórica, Javier; Satrústegui, Jorgina

doi:10.1016/0005-2728(88)90227-7

Cited by 12 publications

(1 citation statement)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…There are several hypotheses to explain the Crabtree eect that do not involve PK. For example, SaÂ nches OlavarrõÂ a et al 28 suggested that a limiting factor in the Crabtree eect is the availability of respiratory substrates. This does not apply to our results however, because in all experimental situations described in the present study, an excess of succinate was used.…”

Section: Resultsmentioning

confidence: 99%

Occurrence of the Crabtree effect in HeLa cells

Melo

Stevan-Hancke

Campello

et al. 1998

Cell Biochem. Funct.

View full text Add to dashboard Cite

The occurrence of a Crabtree effect in HeLa cells was detected. Some properties of pyruvate kinase (PK) were also evaluated. Hexose phosphate, triose-phosphate and phosphoenolpyruvate (PEP) significantly decreased the oxygen consumption of digitonin-permeabilized HeLa cells, which were oxidizing succinate. The Crabtree effect promoted by PEP was concentration-dependent and was lowered by an increase of ADP concentration, suggesting a participation of PK. The dependence of fructose-1,6-bisphosphate (FDP) by HeLa cell PK was observed. The PK of HeLa cells was inhibited by L-alanine only in the absence of FDP, while in the presence of the metabolite, an increase in the activity was observed. PK was also inhibited in the presence of L-histidine and L-leucine, while L-serine promoted activation. L-Cysteine and L-phenylalanine also inhibited the PK of HeLa cells. This, together with the sigmoidal character in relation to substrate concentration, suggests the presence of the K-type of PK in HeLa cells.

show abstract

Section: Resultsmentioning

confidence: 99%