Measurement of Input Impedance and Cytoplasmic Resistivity with A Single Microelectrode

Schanne, Otto F.; Ceretti, Elena R. P. de

doi:10.1139/y71-096

Cited by 24 publications

(19 citation statements)

References 7 publications

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“…This method depends on the bridge balance, which may seriously be affected by a change in the microelectrode resistance. Furthermore, the electrode resistance is a function of the ionic strength or ion concentrations in the fluid surrounding the electrode tip (SCHANNE, 1969). We feel that the one microelectrode method is not suitable for measurement of the input resistance under hypertonic condition, since the cell dehydration and consequent changes in the state of the intracellular fluid will decrease the resistance of microelectrode (SCHANNE, 1969).…”

Section: Discussionmentioning

confidence: 99%

Effects of hypertonic solution on action potential and input resistance in the guinea-pig ventricular muscle.

Ehara

Hasegawa

1983

Jpn.J.Physiol

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The effects of hypertonic solution on action potential and input resistance were studied using the guinea-pig ventricular muscle. Hypertonic solutions containing excess (150 and 300 mOsmf liter) sucrose, glucose, NaCI, Na2SO4, or LiCI produced a prolongation of action potential duration (APD) followed by a gradual shortening. These solutions increased the input resistance of the preparation. The prolongation of APD began immediately after the onset of perfusion with hypertonic solution and peaked within about 10 min, while the late shortening of APD developed progressively. On introducing the isotonic solution after the osmotic challenge, APD initially shortened and then gradually prolonged toward the control level. Slightly hypertonic solutions containing excess 30 or 75 mM sucrose prolonged APD, to some extent. It is concluded that the hypertonic solution exerts early and late effects on APD in guinea-pig ventricular muscle. The early effect, which is responsible for the APD prolongation, seems to be directly related to the development of cell dehydration. The hypertonic solutions produced a slight increase in the resting potential, and sucrose-and glucose-hypertonicity depressed the rising phase of the action potential. These effects can be explained by the cell dehydration and associated changes in the intracellular ion concentrations. The increase in input resistance produced by hypertonic solutions was accompanied by an enhanced spatial decay of electrotonic potentials along the muscle bundle, as determined by the two microelectrode method, suggesting that the electrical resistance of the internal current pathway increases under hypertonic conditions.

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Section: Discussionmentioning

confidence: 99%

Effects of hypertonic solution on action potential and input resistance in the guinea-pig ventricular muscle.

Ehara

Hasegawa

1983

Jpn.J.Physiol

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“…While the fact that our present equipment is limited to frequencies below 13 MHz means that it was not possible to measure the entire secondary dispersion, it is quite evident, as may also be clearly seen in the study of Irimajiri et al [36], that the data may still be reasonably well fitted to a single Cole/ Cole locus, on the one hand, but indicate two quite separate dispersions when observed in the complex admittance plane, on the other hand. This general type of finding, which is becoming increasingly widespread as studies of the dielectric behaviour of biological [4,5,15,60,61] and other [17,62,63] system become more sophisticated, illustrates the point that most real systems are not Kronig-Kramers transformable and thus [15,61] that one or more of the assumptions underlying classical linear dielectric theory, particularly that of local equilibrium, are inapplicable.…”

Section: When Dielectric Measurementsmentioning

confidence: 97%

On the audio- and radio-frequency dielectric behaviour of anchorage-independent, mouse L929-derived LS fibroblasts

Davey¹,

Kell²,

Кемп³

et al. 1988

Bioelectrochemistry and Bioenergetics

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“…Roughly, these protein effects combine to make the cell membrane approximately 10 6 times more conductive to ions than the pure lipid bilayer. 2 The cell membrane is in essence a twodimensional structured fluid, held intact only by van der Waals, hydrophobic, hydrogen-bonding, and screened electrostatic interactions. Occasionally, small separations in the lipid packing order occur, producing transient structural defects with lifetimes on the order of nanoseconds.…”

Section: Barrier Function Of the Cell Membranementioning

confidence: 99%

Triblock Copolymer as an Effective Membrane-Sealing Material

Wu¹,

Frey²,

Lee³

2006

MRS Bull.

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An intact cell membrane serves as a permeable barrier, regulating the influx and efflux of ions and small molecules. When the integrity of the membrane is compromised, its barrier function is also disrupted, threatening the survival of the cell. Triblock copolymer surfactants of the form poly(ethylene oxide)-poly(propylene oxide)-poly(ethylene oxide) have been shown to help seal structurally damaged membranes, arresting the leakage of intracellular materials.In order to understand how this particular family of triblock copolymers helps seal damaged membranes, model lipid monolayer and bilayer systems have been used to unravel the nature of the lipid/copolymer interaction. The copolymer surfactant is found to selectively insert into structurally compromised membranes, thus localizing its sealing effect on the damaged regions. The inserted polymer is "squeezed out" when the lipid packing density is increased, suggesting a mechanism for the cell to be rid of the polymer when the membrane integrity is restored.

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Measurement of Input Impedance and Cytoplasmic Resistivity with A Single Microelectrode

Cited by 24 publications

References 7 publications

Effects of hypertonic solution on action potential and input resistance in the guinea-pig ventricular muscle.

Effects of hypertonic solution on action potential and input resistance in the guinea-pig ventricular muscle.

On the audio- and radio-frequency dielectric behaviour of anchorage-independent, mouse L929-derived LS fibroblasts

Triblock Copolymer as an Effective Membrane-Sealing Material

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