Measurement of Insulin in Human Sera Using a New RIA Kit. 1. Insulin Determination in the Absence of Insulin Antibodies — Conventional Assay and Micro Modification<sup>2</sup>)

Besch, W; Woltanski, K.-P.; Keilacker, H; Díaz-Alonso, Javier; Schulz, B; Amendt, Peter; Kohnert, Klaus–Dieter; Ziegler, M

doi:10.1055/s-0029-1210700

Cited by 29 publications

(15 citation statements)

References 2 publications

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“…In order to check the hypoglycemic effects of NSFO and NSEO, we determined the glucose concentration of individual rat in each study using GOD-PAP method as described by [16], while insulin level was determined following the method of Besch et al [17]. …”

Section: Methodsmentioning

confidence: 99%

Nigella sativa Fixed and Essential Oil Supplementation Modulates Hyperglycemia and Allied Complications in Streptozotocin‐Induced Diabetes Mellitus

Sultan

Butt

Karim

et al. 2014

Evidence-Based Complementary and Alternative Medicine

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In the recent era, diabetes mellitus has emerged as one of the significant threats to public health and this situation demands the attention of the researchers and allied stakeholders. Dietary regimens using functional and nutraceutical foods are gaining wide range of acceptance and some traditional medicinal plants are of considerable importance. The main objective of this instant study was to explore the antidiabetic potential of Nigella sativa fixed oil (NSFO) and essential oil (NSEO). Three experimental groups of rats received diets during the entire study duration, that is, D1 (control), D2 (NSFO: 4.0%), and D3 (NSEO: 0.30%). Experimental diets (NSFO & NSEO) modulated the lipid profile, while decreasing the antioxidant damage. However, production of free radicals, that is, MDA, and conjugated dienes increased by 59.00 and 33.63%, respectively, in control. On the contrary, NSFO and NSEO reduced the MDA levels by 11.54 and 26.86% and the conjugated dienes levels by 32.53 and 38.39%, respectively. N. sativa oils improved the health and showed some promising anti-diabetic results.

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Section: Methodsmentioning

confidence: 99%

Nigella sativa Fixed and Essential Oil Supplementation Modulates Hyperglycemia and Allied Complications in Streptozotocin‐Induced Diabetes Mellitus

Sultan

Butt

Karim

et al. 2014

Evidence-Based Complementary and Alternative Medicine

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“…Plasma insulin was measured by radioimmunoassay 23. Homeostatic model assessment of insulin resistance (HOMAir) index was calculated 24.…”

Section: Methodsmentioning

confidence: 99%

DASH lowers blood pressure in obese hypertensives beyond potassium, magnesium and fibre

et al. 2009

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The mechanism underlying blood pressure reduction in the high fruits and vegetables arm of the Dietary Approaches to Stop Hypertension (DASH) Study is unknown but may include potassium, magnesium and fiber. This study was designed to separate minerals and fiber from other components of DASH on blood pressure in abdominally obese metabolic syndrome subjects with prehypertension to Stage 1 hypertension (obese hypertensives). Fifteen obese hypertensives and 15 lean normotensives were studied on a standardized usual diet, randomized to DASH or usual diet supplemented with potassium, magnesium and fiber to match DASH, then crossed over to the complementary diet. All diets were three weeks long, isocaloric and matched for sodium and calcium. In obese hypertensives, blood pressure was lower after 3 weeks on DASH than usual diet (-7.6±1.4/-5.3±1.4 mmHg, p<0.001/0.02 and usual diet supplemented (-6.2±1.4/-3.7±1.4 p<0.005/0.06), whereas blood pressure was not significantly different on usual and supplemented diets. Blood pressure values were not different among the three diets in lean normotensives. Small artery elasticity was lower in obese hypertensives than lean normotensives on the usual and supplemented diets (p<0.02). This index of endothelial function improved in obese hypertensives (p<0.02) but not lean normotensives on DASH and was no longer different from values in lean normotensives (p>0.50). DASH is more effective than potassium, magnesium, and fiber supplements for lowering blood pressure in obese hypertensives, which suggest that high fruits and vegetables DASH lowers blood pressure and improves endothelial function in this group by nutritional factors in addition to potassium, magnesium and fiber.

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“…Plasma insulin was measured by radioimmunoassay 24. The homeostatic model assessment of insulin resistance (HOMAir) was calculated 25.…”

Section: Methodsmentioning

confidence: 99%

Low-sodium DASH reduces oxidative stress and improves vascular function in salt-sensitive humans

et al. 2009

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Salt induces oxidative stress in salt-sensitive (SS) animals and man. It is unknown in SS subjects if the low-sodium Dietary Approaches to Stop Hypertension (LS-DASH) reduces oxidative stress more than DASH, which is high in anti-oxidants. To assess the effects of DASH and LS-DASH on oxidative stress, 19 volunteers were studied after 3-weeks of a standardized usual low fruits and vegetables diet (ULFV), followed by 3-weeks on DASH (both diets ∼120 mmol Na+/day), then 3-weeks on low-sodium (LS)-DASH (60 mmol Na+/d). SS was defined as systolic blood pressure ≥5 mmHg lower on LS-DASH than DASH. In SS subjects (N=9), systolic blood pressure was lower on LS-DASH (111.0±2.0 mmHg) than DASH (118.0±2.2, p<0.01) and ULFV (122.3±2.7, p=0.002). In salt-resistant (SR) volunteers (N=10), systolic blood pressure was lower on DASH (113.0±1.6) than ULFV (119.0±1.8, p<0.05) but not LS-DASH (115.7±1.8). Urine F2-isoprostanes, a marker of oxidative stress, were lower in SS subjects on LS-DASH (1.69±0.24) than ULFV (3.09±0.50, p<0.05) and marginally lower than DASH (2.46±0.44, p<0.20). F2-isoprostanes were not different among the three diets in SR volunteers (2.18±0.29, 2.06±0.29, 2.27±0.53, respectively). Aortic augmentation index, a measure of vascular stiffness, was lower in SS subjects on LS-DASH than either DASH or ULFV, and lower on DASH than ULFV in SR volunteers. In SS but not SR subjects, LS-DASH is associated with lower values for F2-isorprostane and the aortic augmentation index. The results suggest that LS-DASH decreases oxidative stress, improves vascular function and lowers blood pressure in SS but not SR volunteers.

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Measurement of Insulin in Human Sera Using a New RIA Kit. 1. Insulin Determination in the Absence of Insulin Antibodies — Conventional Assay and Micro Modification²)

Cited by 29 publications

References 2 publications

Nigella sativa Fixed and Essential Oil Supplementation Modulates Hyperglycemia and Allied Complications in Streptozotocin‐Induced Diabetes Mellitus

Nigella sativa Fixed and Essential Oil Supplementation Modulates Hyperglycemia and Allied Complications in Streptozotocin‐Induced Diabetes Mellitus

DASH lowers blood pressure in obese hypertensives beyond potassium, magnesium and fibre

Low-sodium DASH reduces oxidative stress and improves vascular function in salt-sensitive humans

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Measurement of Insulin in Human Sera Using a New RIA Kit. 1. Insulin Determination in the Absence of Insulin Antibodies — Conventional Assay and Micro Modification2)

Cited by 29 publications

References 2 publications

Nigella sativa Fixed and Essential Oil Supplementation Modulates Hyperglycemia and Allied Complications in Streptozotocin‐Induced Diabetes Mellitus

Nigella sativa Fixed and Essential Oil Supplementation Modulates Hyperglycemia and Allied Complications in Streptozotocin‐Induced Diabetes Mellitus

DASH lowers blood pressure in obese hypertensives beyond potassium, magnesium and fibre

Low-sodium DASH reduces oxidative stress and improves vascular function in salt-sensitive humans

Contact Info

Product

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Measurement of Insulin in Human Sera Using a New RIA Kit. 1. Insulin Determination in the Absence of Insulin Antibodies — Conventional Assay and Micro Modification²)