Measurement of interleukin-6 (IL-6) and erythropoietin (EPO) in umbilical cords of preterm infants with intraventricular hemorrhage in two hospitals in Tehran

Khosravi, Nastaran; Badamchi, Ali; Khalesi, Nasrin; Tabatabaee, A; Naghdalipour, Mehri; Asgarian, Ramin

doi:10.1080/14767058.2016.1228055

Cited by 6 publications

(4 citation statements)

References 14 publications

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“…Second, our current study, like many of the studies on human recurrent pregnancy loss, has still not demonstrated a direct cause-and-effect relationship between the inflammatory event and pregnancy loss; for instance, an inflammatory maternal response to a fetus that has died due to non-immunological factors, could well manifest the IL-6 secretion in CD4+ T cells and/or the production of other inflammatory cytokines at the maternal–fetal interface. However, given the association between IL-6 dysregulation and pregnancy failure, and the previously demonstrated deleterious effects of IL-6 on the conceptus and the fetal [ 41 , 42 ], it is suggested that IL-6-mediated effects may have been the cause of pregnancy failure in at least a proportion of the cases. Additional studies will be needed to confirm causality and determine the mechanism of action.…”

Section: Discussionmentioning

confidence: 99%

The AHNAK induces increased IL-6 production in CD4+ T cells and serves as a potential diagnostic biomarker for recurrent pregnancy loss

Liu

Feng

et al. 2022

Clinical and Experimental Immunology

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Disorganized maternal–fetal immune tolerance contributes to the occurrence of unexplained recurrent pregnancy loss (RPL). AHNAK is a scaffolding protein participating in the regulation of Ca2+ entry into T cells and the pathophysiology of diverse diseases. We performed differential gene expression analysis in decidual immune cells (DICs) isolated from three patients with RPL and from three healthy controls via RNA-sequencing (RNA-seq), which revealed 407 differentially expressed genes (DEGs). Among these DEGs, we underscored the clinical significance of elevated AHNAK mRNA and protein levels in DICs, peripheral blood mononuclear cells (PBMCs), and decidua of the patients with RPL, suggesting its potential use as a biomarker for the diagnosis of RPL. Especially, the ratios of decidual and blood AHNAK+CD4+ T cells in the CD4+ T cell population were significantly increased in patients with RPL, and the loss of AHNAK was further shown to inhibit interleukin (IL)-6 secretion in the CD4+ Jurkat cell line. Similar patterns were also observed in the clinical decidual and blood specimens. We uncovered that the AHNAK+CD4+ T cells could secrete more IL-6 than that the corresponding AHNAK-CD4+ T cells. Moreover, the frequencies of decidual and blood IL-6+CD4+ T cells in the CD4+ T-cell population were also increased in patients with RPL and showed significant positive correlations with the frequencies of AHNAK+CD4+ T cells. Our findings suggest that the elevated AHNAK expressed by CD4+ T cells may be involved in the immune dysregulation of RPL by increasing IL-6 production, illustrating its potential as a novel intervention target for RPL.

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Section: Discussionmentioning

confidence: 99%

The AHNAK induces increased IL-6 production in CD4+ T cells and serves as a potential diagnostic biomarker for recurrent pregnancy loss

Liu

Feng

et al. 2022

Clinical and Experimental Immunology

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“…This speculation is supported by previous reports that the cord serum level of IL-6 is a significant predictor of the severity of the hypoxic-ischemic encephalopathy and IVH in preterm neonates. 24,25 An important finding of this study is that elevated C5a cord plasma levels at birth were independently associated with vision-threatening ROP requiring laser treatment after adjusting for important clinical factors, including gestational age at birth and birth weight. To our knowledge, this is the first study that describes changes in cord plasma levels of complement activation fragments at birth in relation to the postnatal development of ROP.…”

Section: Discussionmentioning

confidence: 75%

Immune and Inflammatory Proteins in Cord Blood as Predictive Biomarkers of Retinopathy of Prematurity in Preterm Infants

Park

Woo

Kim

et al. 2019

Invest. Ophthalmol. Vis. Sci.

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PURPOSE. To determine whether elevated levels of immune/inflammatory proteins in cord blood, alone or in combination with conventional clinical parameters, can predict the occurrence and progression of retinopathy of prematurity (ROP) in preterm infants. METHODS. This was a retrospective cohort study of 110 premature singleton infants who were born at 32.0 weeks. Cord plasma at birth was assayed for interleukin-6, C3a, C5a, matrix metalloproteinase-2 (MMP-2), MMP-9, tissue inhibitor of metalloproteinase-1, macrophage colony-stimulating factor, endostatin, a proliferation-inducing ligand, insulin-like growth factor-binding protein-1 (IGFBP-1), IGFBP-2, and calcium-binding protein A8/A9 complex levels. The primary outcome measures were the occurrence of any stage ROP, severe ROP (>stage 3), and vision-threatening type 1 ROP requiring laser treatment. RESULTS. ROP was diagnosed in 30 of 110 infants (27.3%), including 14 (12.7%) with severe ROP. Laser treatment was performed on 7 infants (6.4%). Multiple logistic regression analyses indicated that elevated levels of cord plasma IL-6 were significantly associated with severe ROP, whereas elevated levels of cord plasma C5a were significantly associated with ROP laser treatments. However, none of the proteins measured in the cord plasma were associated with ROP occurrence. Using a stepwise regression procedure, we developed a combined prediction model, which included high cord plasma IL-6 levels and low birth weight for severe ROP (area under the curve [AUC], 0.840), and high cord plasma C5a levels and low birth weight for laser treatment (AUC, 0.884). CONCLUSIONS. Elevated levels of cord plasma IL-6 and C5a could be used as independent markers to predict severe ROP and laser treatment, respectively, with combined models predicting ROP progression with good accuracy.

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“…(2012), свидетельствующих о снижении уровня IL-6 в пуповинной крови детей с БЛД тяжелой степени, в проведенном нами исследовании отмечалось его повышение на уровне тенденции. Некоторые авторы связывают высокие уровни IL-6 при рождении с развитием синдрома воспалительной реакции плода и неблагоприятным неонатальным исходом [13], ранними пери/ интравентрикулярными кровоизлияниями [15] и сепсисом у недоношенных детей [16]. По нашему мнению, повышение уровня IL-6 в пуповинной крови может явиться следствием перенесенной гипоксии, внутриутробной инфекции [10].…”

Section: Discussionunclassified

Features of Immune Response in Preterm Babies With Bronchopulmonary Displasia of Various Severity

et al. 2019

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To evaluate the immunity indexes in the children who were born with bronchopulmonary dysplasia (BPD) of varying severity at very early delivery terms (22-27 weeks), dynamic examinations were performed in 35 infants: 17 children were with severe BPD; 18, with mild-severity and moderately severe BPD. The comparison group consisted of seven children born at 22-27 weeks of gestational age without signs of BPD. Relative numbers of lymphocyte subpopulations (CD3+, CD4+, CD8+, CD19+, CD16+CD56+, CD4+CD25+), and monocytes (CD14+CD64+, CD14+HLA-DR+) were determined by flow cytometry. The level of cytokines (IL-6, IL-8, IL-4) was measured by enzyme immunoassay technique. The features of immune status in children with BPD of severe, mild and moderate severity were discerned. It was found that the predictors of severe BPD development in the children born at very early terms, are: increased content of IL-8 at birth and at the age of 1 month, reduced level of expression on monocytes (CD64, HLA-DR) on the 1 month of life, and CD14+CD64+cells at 38-40 weeks post conception. The revealed features of immune status in newborns with BPD can be used to assess the effectiveness of the therapy, which requires further research in this direction.

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Measurement of interleukin-6 (IL-6) and erythropoietin (EPO) in umbilical cords of preterm infants with intraventricular hemorrhage in two hospitals in Tehran

Abstract: The concentration of IL-6 and EPO levels of the cord plasma was higher in the premature newborns with IVH.

Cited by 6 publications

References 14 publications

The AHNAK induces increased IL-6 production in CD4+ T cells and serves as a potential diagnostic biomarker for recurrent pregnancy loss

The AHNAK induces increased IL-6 production in CD4+ T cells and serves as a potential diagnostic biomarker for recurrent pregnancy loss

Immune and Inflammatory Proteins in Cord Blood as Predictive Biomarkers of Retinopathy of Prematurity in Preterm Infants

Features of Immune Response in Preterm Babies With Bronchopulmonary Displasia of Various Severity

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