2000
DOI: 10.1016/s0168-1656(99)00203-5
|View full text |Cite
|
Sign up to set email alerts
|

Measurement of intracellular (compartmental) pH by 31P NMR in Aspergillus niger

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1
1
1
1

Citation Types

3
56
0

Year Published

2001
2001
2021
2021

Publication Types

Select...
8
1

Relationship

0
9

Authors

Journals

citations
Cited by 123 publications
(59 citation statements)
references
References 23 publications
3
56
0
Order By: Relevance
“…Our study revealed that the cytoplasmic pH in young hyphae of A. niger is tightly regulated at pH ϳ7.6. This estimate is in agreement with intracellular pH measurements obtained with 31 P-NMR (19,36), but it was 0.2 pH units higher when measurements were made with the ratiometric fluorescent dye SNARF (24). However, in the latter study an esterified version of the SNARF dye was used that tends to sequester within intracellular organelles (34).…”
Section: Discussionsupporting
confidence: 82%
See 1 more Smart Citation
“…Our study revealed that the cytoplasmic pH in young hyphae of A. niger is tightly regulated at pH ϳ7.6. This estimate is in agreement with intracellular pH measurements obtained with 31 P-NMR (19,36), but it was 0.2 pH units higher when measurements were made with the ratiometric fluorescent dye SNARF (24). However, in the latter study an esterified version of the SNARF dye was used that tends to sequester within intracellular organelles (34).…”
Section: Discussionsupporting
confidence: 82%
“…Intracellular pH measurements with free pH-sensitive dyes often suffer from problems associated with dye loading and dye sequestration within organelles (21,33). There are also reports on the use of radiolabeled membranepermeable acids (3) and 31 P nuclear magnetic resonance (NMR) for intracellular pH measurement (18,19,20) in fungi. However, both of these methods require extensive sample manipulation and do not allow the imaging of intracellular pH in single living cells.…”
mentioning
confidence: 99%
“…4). The optimal pH is between 7n5 and 8n0 which is close to the pH of the Aspergillus cytosol of pH 7n8 (Hesse et al, 2000). This slightly differs from the optimal pH of LAPI (pH 7n5) (Trumbly & Bradley, 1983).…”
Section: Substratementioning
confidence: 77%
“…Immobilization of A. niger NW131 conidia in Ca 2ϩ alginate beads (Manugel DJX; ISP Alginates, Tadworth, United Kingdom) and subsequent culturing were performed as described previously (14).…”
Section: Methodsmentioning
confidence: 99%