Measurement of ion leakage from plant cells in response to aquatic pollutants

Koch, Uwe; Glatzle, D; Ringenbach, F.; Dunz, Thomas; Steger‐Hartmann, Thomas; Wagner, Edgar

doi:10.1007/bf00192606

Cited by 7 publications

(3 citation statements)

References 2 publications

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“…Before the r-BuOOHderived radicals could be detoxified by intracellu lar antioxidants, they would lead to oxidation of the plasmalemma or intrinsic plasma m em brane pro teins. In turn, this oxidation causes ion leakage from the damaged cell [20,30]. In contrast, chloroplastderived 0 2~ faces the intracellular antioxidative sys tem (including rutin), before it causes an oxidation of the plasmalemma.…”

Section: Discussionmentioning

confidence: 99%

Induced Accumulation and Potential Antioxidative Function of Rutin in Two Cultivars of Nicotiana tabacum L.

Steger‐Hartmann

Koch

Dunz

et al. 1994

Zeitschrift Für Naturforschung C

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The rutin (quercetin-3-rhamnosyl-glucoside) content of two tobacco cultivars (Nicotiana tabacum L.) which differ in their ozone-sensitivity was assayed after exposure to various rutininducing stimuli. In the growth-chamber, UV radiation in combination with white light led to the accumulation of similar amounts of rutin in both cultivars. Treatment with radical producing agents (tert-butylhydroperoxide and paraquat) also led to rutin accumulation. In this case, the rutin content was higher in the tolerant cultivar. The rutin content was also higher in the tolerant cultivar upon exposure of the plants on an out-door stand, even when the UV-part of the sun spectrum was excluded by cut-off filters. The potential role of rutin as antioxidant was tested with an ion leakage assay. Plants with relatively high rutin content were less sensitive towards paraquat-induced ion leakage than plants without rutin. Thus, the higher rutin content of the ozone-tolerant cultivar Bel B may well contribute to its tolerance against oxidative stress.

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Section: Discussionmentioning

confidence: 99%

Induced Accumulation and Potential Antioxidative Function of Rutin in Two Cultivars of Nicotiana tabacum L.

Steger‐Hartmann

Koch

Dunz

et al. 1994

Zeitschrift Für Naturforschung C

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“…Ion leakage, considered to be a useful indicator of damage to the plasma-membrane (Koch et al 1995), was determined using the protocol described in Brown and Newman (2003). Following exposure to 0, 10, 20, 30 and 40 mg l -1 CAPB in seawater for either 48 h or 120 h, individual discs were transferred to boiling tubes containing 5 ml of ultra-pure water, left for 5 min (Sample 1) and again transferred to a second set of boiling tubes containing 5 ml of ultra-pure water that was boiled for 5 min (Sample 2), after which the discs were discarded.…”

Section: Exposure To Capbmentioning

confidence: 99%

Toxicity of the amphoteric surfactant, cocamidopropyl betaine, to the marine macroalga, Ulva lactuca

2010

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The degradation of the synthetic, amphoteric surfactant, cocamidopropyl betaine (CAPB) and its toxicity to the marine macroalga, Ulva lactuca, has been evaluated using several different physiological test end-points over different periods of exposure up to 120 h. Droplet surface angle measurements revealed that, following a period of acclimation of about 24 h, CAPB began to degrade and that primary degradation was complete within 120 h. Effective quantum yield (∆F/F(m)') and relative growth rates (RGRs) were the most sensitive measures of phytotoxicity, with CAPB concentrations at and above 10 mg l(-1) eliciting irreversible, time-dependent and/or dose-dependent responses. Cell membrane damage, estimated from measurements of ion leakage, was detected only at a concentration of 40 mg l(-1) after 48 h of exposure to CAPB but by 120 h damage was evident at all measured concentrations above 10 mg l(-1). These observations suggest that both CAPB and its metabolites are intrinsically toxic to U. lactuca. The findings of this study are discussed in terms of the environmental consequences of applying CAPB to control harmful algal blooms.

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“…Particularly in the "eld of plant physiology, damage due to dehydration (Pearce, 1985), freezing (Caphorn et al, 1994;Murray et al, 1989;Sule et al, 1991) or cooling (Pearce and Willison, 1985;Ste!en et al, 1989), air pollution (Tarhanen et al, 1996), water stress, or oxidative stress (Keitel and Arndt, 1983;Koch et al, 1993Koch et al, , 1995StaK dtler and Ziegler, 1993) is frequently detected by measuring the loss of substances from cells into a surrounding medium. The released substances are detected either by spectroscopy (betacyanin, UV-active compounds) or by conductometry (electrolytes).…”

Section: Introductionmentioning

confidence: 99%