Measurement of LIII jump ratio, jump factor and related parameters for Pt and Tl

Singh, Gurinderjeet; Singh, Amrit; Gupta, Manoj Kumar; Kahlon, K.S.; Dhaliwal, A.S.

doi:10.1016/j.jallcom.2015.09.062

Cited by 2 publications

(1 citation statement)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We also included methylated pomalidomide (which lacks CRBN binding affinity) and eight compounds known to induce diverse morphological changes (dubbed "morphological controls") recommended by the Joint Undertaking in Morphological Profiling-Cell Painting consortium. 30 CRBN-Dependent Degraders Have Different Morphological Perturbation Strengths. Prior to screening the compound library in the isogenic CPA, we assessed their CRBN binding affinities using a fluorescence-polarizationbased competition assay with recombinant DDB1-CRBN and the fluorescent tracer 16 (Figure S2).…”

Section: ■ Resultsmentioning

confidence: 99%

Discovery of Molecular Glue Degraders via Isogenic Morphological Profiling

Ng,

Offensperger,

Cisneros

et al. 2023

ACS Chem. Biol.

View full text Add to dashboard Cite

Molecular glue degraders (MGDs) are small molecules that degrade proteins of interest via the ubiquitin–proteasome system. While MGDs were historically discovered serendipitously, approaches for MGD discovery now include cell-viability-based drug screens or data mining of public transcriptomics and drug response datasets. These approaches, however, have target spaces restricted to the essential proteins. Here we develop a high-throughput workflow for MGD discovery that also reaches the nonessential proteome. This workflow begins with the rapid synthesis of a compound library by sulfur(VI) fluoride exchange chemistry coupled to a morphological profiling assay in isogenic cell lines that vary in levels of the E3 ligase CRBN. By comparing the morphological changes induced by compound treatment across the isogenic cell lines, we were able to identify FL2-14 as a CRBN-dependent MGD targeting the nonessential protein GSPT2. We envision that this workflow would contribute to the discovery and characterization of MGDs that target a wider range of proteins.

show abstract

Section: ■ Resultsmentioning

confidence: 99%

Discovery of Molecular Glue Degraders via Isogenic Morphological Profiling

Ng,

Offensperger,

Cisneros

et al. 2023

ACS Chem. Biol.

View full text Add to dashboard Cite

show abstract

Determination of L_III subshell photoelectric parameters of iridium

Cengi̇z

2017

Can. J. Phys.

View full text Add to dashboard Cite

The LIII subshell photoelectric cross section, jump ratio, jump factor, and Davisson–Kirchner ratio of iridium have been determined by mass attenuation coefficients. The measurements have been performed using the X-ray attenuation method in narrow beam geometry. The obtained results have been compared with the tabulated values of XCOM (Berger et al. XCOM: Photon cross section database (version 1.3). NIST. Available at http://physics.nist.gov/xcom . 2005) and FFAST (Chantler et al. X-ray form factor, attenuation and scattering tables (version 2.1). NIST. Available at http://physics.nist.gov/ffast . 2005).

show abstract

Measurement of LIII jump ratio, jump factor and related parameters for Pt and Tl

Cited by 2 publications

References 12 publications

Discovery of Molecular Glue Degraders via Isogenic Morphological Profiling

Discovery of Molecular Glue Degraders via Isogenic Morphological Profiling

Determination of L_III subshell photoelectric parameters of iridium

Contact Info

Product

Resources

About

Measurement of LIII jump ratio, jump factor and related parameters for Pt and Tl

Cited by 2 publications

References 12 publications

Discovery of Molecular Glue Degraders via Isogenic Morphological Profiling

Discovery of Molecular Glue Degraders via Isogenic Morphological Profiling

Determination of LIII subshell photoelectric parameters of iridium

Contact Info

Product

Resources

About

Determination of L_III subshell photoelectric parameters of iridium