Measurement of Lower Limb Blood Flow in Patients With Neurogenic Claudication Using Positron Emission Tomography

Keenan, Gary F.; Ashcroft, GP; Roditi, Giles; Hutchison, J.M.S.; Evans, N.; Mikecz, Pál; Chaloner, F.; Dodd, M.; Leonard, Christopher; Porter, Richard W.; Sharp, P.; Smith, F. W.

doi:10.1097/00007632-199502001-00002

Cited by 6 publications

(4 citation statements)

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“…Similarly, PET imaging with small molecules such as 13 N-NH 3 or 15 O-H 2 O to measure extremity blood flow does not assess molecular changes either. 23, 27–29 Laser Doppler perfusion imaging is frequently used to examine the hindlimb perfusion. This technique, however, is limited to measurement of superficial blood flow and is unlikely to provide reliable information about the subtle changes in global blood flow.…”

Section: Discussionmentioning

confidence: 99%

“…High-resolution MR angiography and spectroscopy, as well as CT angiography, are newer vascular imaging strategies that are less invasive than the conventional techniques; however they cannot detect molecular changes occurring early in the disease process. Similarly, PET imaging with small molecules such as 13 N-NH 3 or 15 O–H 2 O to measure extremity blood flow does not assess molecular changes either. ,− Laser Doppler perfusion imaging is frequently used to examine the hindlimb perfusion. This technique, however, is limited to measurement of superficial blood flow and is unlikely to provide reliable information about the subtle changes in global blood flow.…”

Section: Discussionmentioning

confidence: 99%

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Positron Emission Tomography Imaging of Angiogenesis in a Murine Hindlimb Ischemia Model with ⁶⁴Cu-Labeled TRC105

Orbay

Zhang²,

Hong³

et al. 2013

Mol. Pharmaceutics

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The goal of this study was to assess ischemia-induced angiogenesis with 64Cu-NOTA-TRC105 positron emission tomography (PET) in a murine hindlimb ischemia model of peripheral artery disease (PAD). CD105 binding affinity/specificity of NOTA-conjugated TRC105 (an anti-CD105 antibody) was evaluated by flow cytometry, which exhibited no difference from unconjugated TRC105. BALB/c mice were anesthetized and the right femoral artery was ligated to induce hindlimb ischemia, with the left hindlimb serving as an internal control. Laser Doppler imaging showed that perfusion in the ischemic hindlimb plummeted to ~20% of the normal level after surgery, and gradually recovered to near normal level on day 24. Ischemia-induced angiogenesis was non-invasively monitored and quantified with 64Cu-NOTA-TRC105 PET on postoperative days 1, 3, 10, 17, & 24. 64Cu-NOTA-TRC105 uptake in the ischemic hindlimb increased significantly from the control level of 1.6±0.2 %ID/g to 14.1±1.9 %ID/g at day 3 (n=3), and gradually decreased with time (3.4±1.9 %ID/g at day 24), which correlated well with biodistribution studies performed on days 3 & 24. Blocking studies confirmed the CD105 specificity of tracer uptake in the ischemic hindlimb. Increased CD105 expression on days 3 and 10 following ischemia was confirmed by histology and RT-PCR. This is the first report of PET imaging of CD105 expression during ischemia-induced angiogenesis. 64Cu-NOTA-TRC105 PET may play multiple roles in future PAD-related research and improve PAD patient management by identifying the optimal timing of treatment and monitoring the efficacy of therapy.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Positron Emission Tomography Imaging of Angiogenesis in a Murine Hindlimb Ischemia Model with ⁶⁴Cu-Labeled TRC105

Orbay

Zhang²,

Hong³

et al. 2013

Mol. Pharmaceutics

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“…In general, PET imaging of extremity ischemia in human subjects aims to quantify the extremity blood flow and O 2 utilization using C 15 O 2 or 15 O 2 as the tracer [50–52]. In patients with unilateral neurogenic claudication, 15 O-labeled water has also been used to measure thigh and leg muscle blood flow with PET in response to exercise [52].…”

Section: Clinical Imaging Of Extremity Ischemiamentioning

confidence: 99%

“…In patients with unilateral neurogenic claudication, 15 O-labeled water has also been used to measure thigh and leg muscle blood flow with PET in response to exercise [52]. In another study, H 2 15 O PET scans were carried out in 17 patients to detect the muscle blood flow of legs with severe ischemia [53].…”

Section: Clinical Imaging Of Extremity Ischemiamentioning

confidence: 99%

PET/SPECT imaging of hindlimb ischemia: focusing on angiogenesis and blood flow

et al. 2012

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Peripheral artery disease (PAD) is a result of the atherosclerotic narrowing of blood vessels to the extremities, and the subsequent tissue ischemia can lead to the up-regulation of angiogenic growth factors and formation of new vessels as a recovery mechanism. Such formation of new vessels can be evaluated with various non-invasive molecular imaging techniques, where serial images from the same subjects can be obtained to allow the documentation of disease progression and therapeutic response. The most commonly used animal model for preclinical studies of PAD is the murine hindlimb ischemia model, and a number of radiotracers have been investigated for positron emission tomography (PET) and single photon emission computed tomography (SPECT) imaging of PAD. In this review article, we summarize the PET/SPECT tracers that have been tested in the murine hindlimb ischemia model as well as those used clinically to assess the extremity blood flow.

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The stenotic concept

Ombregt

2013

A System of Orthopaedic Medicine

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Measurement of Lower Limb Blood Flow in Patients With Neurogenic Claudication Using Positron Emission Tomography

Cited by 6 publications

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Positron Emission Tomography Imaging of Angiogenesis in a Murine Hindlimb Ischemia Model with ⁶⁴Cu-Labeled TRC105

Positron Emission Tomography Imaging of Angiogenesis in a Murine Hindlimb Ischemia Model with ⁶⁴Cu-Labeled TRC105

PET/SPECT imaging of hindlimb ischemia: focusing on angiogenesis and blood flow

The stenotic concept

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Measurement of Lower Limb Blood Flow in Patients With Neurogenic Claudication Using Positron Emission Tomography

Cited by 6 publications

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Positron Emission Tomography Imaging of Angiogenesis in a Murine Hindlimb Ischemia Model with 64Cu-Labeled TRC105

Positron Emission Tomography Imaging of Angiogenesis in a Murine Hindlimb Ischemia Model with 64Cu-Labeled TRC105

PET/SPECT imaging of hindlimb ischemia: focusing on angiogenesis and blood flow

The stenotic concept

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Product

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Positron Emission Tomography Imaging of Angiogenesis in a Murine Hindlimb Ischemia Model with ⁶⁴Cu-Labeled TRC105

Positron Emission Tomography Imaging of Angiogenesis in a Murine Hindlimb Ischemia Model with ⁶⁴Cu-Labeled TRC105