Measurement of microcirculation in the optic nerve head by laser speckle flowgraphy and scanning laser doppler flowmetry

Yaoeda, Kiyoshi; Shirakashi, Masataka; Funaki, Shigeo; Funaki, Haruko; Nakatsue, Takeshi; Abe, Haruki

doi:10.1016/s0002-9394(00)00382-2

Cited by 83 publications

(53 citation statements)

References 17 publications

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“…However, both repeatability and reproducibility have been considered problematic with all techniques used to date thus limiting their value in clinical practice as well as in clinical trials. 11,17,18,[20][21][22][23] In our study, using the OCTA with OMAG technique for quantifying ONH perfusion, the intravisit and intervisit repeatability were ≤3.3% and ≤4.4% (CVs for the full ONH). Our results provide ONH perfusion measurements with higher repeatability and reproducibility values than those from other reports of noninvasive imaging Table 1 Intravisit repeatability of ONH perfusion metrics (flux, vessel area density, and normalized flux) using OCTA in normal eyes.…”

Section: Discussionmentioning

confidence: 86%

“…1 Such diseases include: glaucoma, [2][3][4][5] age-related macular degeneration, 6 and diabetic retinopathy. 7 Over the past several decades, various imaging techniques, such as fluorescein angiography, 8 laser Doppler flowmeter (LDF), 2,9,10 scanning laser Doppler flowmetry (SLDF), [11][12][13][14][15][16] laser speckle flowgraphy (LSFG), 17,18 and Doppler optical coherence tomography (D-OCT), 19 have been used to directly measure the ocular blood flow and vascular volume, or to indirectly quantify the parameters via perfusion time, flow velocity, flux of the blood cells, or vessel diameter changes.…”

Section: Introductionmentioning

confidence: 99%

“…They reported that the CVs for intervisit repeatability of the parameters ranged from 9.7% to 19.8%. 17,18 We previously developed a noninvasive imaging technique capable of providing three-dimensional in vivo blood flow visualization within microcirculatory tissue beds in the eye. The imaging approach uses a novel optical microangiography (OMAG) technique based on Fourier-domain optical coherence tomography (FD-OCT) systems.…”

Section: Introductionmentioning

confidence: 99%

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Repeatability and reproducibility of optic nerve head perfusion measurements using optical coherence tomography angiography

et al. 2016

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of optic nerve head perfusion measurements using optical coherence tomography angiography," J. Biomed. Opt. 21(6), 065002 (2016), doi: 10.1117/1.JBO.21.6.065002. Abstract. Optical coherence tomography angiography (OCTA) has increasingly become a clinically useful technique in ophthalmic imaging. We evaluate the repeatability and reproducibility of blood perfusion in the optic nerve head (ONH) measured using optical microangiography (OMAG)-based OCTA. Ten eyes from 10 healthy volunteers are recruited and scanned three times with a 68-kHz Cirrus HD-OCT 5000-based OMAG prototype system (Carl Zeiss Meditec Inc., Dublin, California) centered at the ONH involving two separate visits within six weeks. Vascular images are generated with OMAG processing by detecting the differences in OCT signals between consecutive B-scans acquired at the same retina location. ONH perfusion is quantified as flux, vessel area density, and normalized flux within the ONH for the prelaminar, lamina cribrosa, and the full ONH. Coefficient of variation (CV) and intraclass correlation coefficient (ICC) are used to evaluate intravisit and intervisit repeatability, and interobserver reproducibility. ONH perfusion measurements show high repeatability [CV ≤ 3.7% (intravisit) and ≤ 5.2% (intervisit)] and interobserver reproducibility (ICC ≤ 0.966) in all three layers by three metrics. OCTA provides a noninvasive method to visualize and quantify ONH perfusion in human eyes with excellent repeatability and reproducibility, which may add additional insight into ONH perfusion in clinical practice. © The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

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Section: Discussionmentioning

confidence: 86%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Repeatability and reproducibility of optic nerve head perfusion measurements using optical coherence tomography angiography

et al. 2016

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“…Applications in the retina have included analysis of the effects of a wide range of pharmacological agents on blood flow 43,44 as well as analysis of flow around the optic nerve head. 45 Despite the large number of reports of the use of speckle techniques for measuring retinal blood flow, however, very few images of retinal blood flow in humans have been reported. The majority of studies have reported average flow values that are calculated from images without showing any spatial maps of blood flow.…”

Section: Retinal Blood Flowmentioning

confidence: 99%

Laser speckle contrast imaging in biomedical optics

Boas¹,

2010

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Abstract. First introduced in the 1980s, laser speckle contrast imaging is a powerful tool for full-field imaging of blood flow. Recently laser speckle contrast imaging has gained increased attention, in part due to its rapid adoption for blood flow studies in the brain. We review the underlying physics of speckle contrast imaging and discuss recent developments to improve the quantitative accuracy of blood flow measures. We also review applications of laser speckle contrast imaging in neuroscience, dermatology and ophthalmology.

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“…The methodologies used to obtain ocular blood flow measurements can involve both invasive and noninvasive techniques. For example, the dye dilution method 6 is an invasive technique, while color Doppler imaging, 7 laser Doppler velocimetry, 8 laser speckle flowgraphy (LSFG) (LSFG-Navi, Softcare, Fukuoka, Japan), [9][10][11][12][13][14][15][16] and the retinal functional imager methodologies 17 are considered to be noninvasive techniques. The LSFG device has been used to visualize blood flow distributions in the ocular fundus.…”

Section: Central Retinal Vein Occlusion (Crvo) Is An Ophthalmologic Vmentioning

confidence: 99%

Retinal Blood Flow Correlates to Aqueous Vascular Endothelial Growth Factor in Central Retinal Vein Occlusion

Yamada

Suzuma

Matsumoto

et al. 2015

Retina

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Results: Fluorescein angiography found 20 ischemic and 25 non-ischemic type eyes. Aqueous VEGF concentration in the ischemic type was significantly higher than that in the non-ischemic type (P = 0.01). Arteriovenous passage time was significantly correlated to the logarithm of the aqueous VEGF concentration (P = 0.0001). MBR of the affected eye / MBR of the unaffected eye of the ischemic type was significantly lower than the non-ischemic type (P = 0.039). Additionally, MBR was significantly correlated both to the logarithm of the aqueous VEGF concentration (P < 0.0001) and to the arteriovenous passage time (P = 0.0001).

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Measurement of microcirculation in the optic nerve head by laser speckle flowgraphy and scanning laser doppler flowmetry

Cited by 83 publications

References 17 publications

Repeatability and reproducibility of optic nerve head perfusion measurements using optical coherence tomography angiography

Repeatability and reproducibility of optic nerve head perfusion measurements using optical coherence tomography angiography

Laser speckle contrast imaging in biomedical optics

Retinal Blood Flow Correlates to Aqueous Vascular Endothelial Growth Factor in Central Retinal Vein Occlusion

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