2020
DOI: 10.3390/membranes10030043
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Measurement of Multi Ion Transport through Human Bronchial Epithelial Cell Line Provides an Insight into the Mechanism of Defective Water Transport in Cystic Fibrosis

Abstract: We measured concentration changes of sodium, potassium, chloride ions, pH and the transepithelial potential difference by means of ion-selective electrodes, which were placed on both sides of a human bronchial epithelial 16HBE14σ cell line grown on a porous support in the presence of ion channel blockers. We found that, in the isosmotic transepithelial concentration gradient of either sodium or chloride ions, there is an electroneutral transport of the isosmotic solution of sodium chloride in both directions a… Show more

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Cited by 9 publications
(10 citation statements)
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“…Over the past few decades, we have witnessed the development of ISEs from LC to SC that has miniaturized and integrated the ISEs. In addition to the wearable sensors, SC-ISEs could be further intensively explored for in vivo investigations at cell, tissue or organ level [103][104][105][106][107]. More scientific challenges and the promise of practical application provide tremendous space and the next hot-spot for the SC-ISEs.…”
Section: Discussionmentioning
confidence: 99%
“…Over the past few decades, we have witnessed the development of ISEs from LC to SC that has miniaturized and integrated the ISEs. In addition to the wearable sensors, SC-ISEs could be further intensively explored for in vivo investigations at cell, tissue or organ level [103][104][105][106][107]. More scientific challenges and the promise of practical application provide tremendous space and the next hot-spot for the SC-ISEs.…”
Section: Discussionmentioning
confidence: 99%
“…Immortalized human bronchial epithelial cells (16HBE14o-) were obtained from Dieter Gruenert from the University of California (San Francisco, CA, USA) (UC Case No. SF1992-A66) [ 2 , 52 ]. Human bronchial epithelial (HBE) cells were cultured in MEM (Sigma-Aldrich, St. Louis, MO, USA) supplemented with 10% FBS (Gibco, Carlsbad, CA, USA), 100 U/mL penicillin (Sigma-Aldrich, St. Louis, MO, USA) and 100 µg/mL streptomycin (Sigma-Aldrich, St. Louis, MO, USA) at 37 °C in a humidified atmosphere with 5% CO 2 [ 2 , 52 ].…”
Section: Methodsmentioning
confidence: 99%
“…The monolayer of HBE cells creates a connection between the external and internal environment in the body, which makes them the main target of inhaled insults [ 1 ]. Epithelial cells are rich in a wide variety of ion transport proteins, among which Na + , Cl − and K + channels have been identified to play a role in the regulation of the fluid layer [ 2 ]. Malfunctions of bronchial epithelial ions transport processes and the subsequent impairment of the liquid balance in the lungs are associated with severe diseases, such as cystic fibrosis and pulmonary edema [ 3 , 4 ].…”
Section: Introductionmentioning
confidence: 99%
“…On the apical surface, CFTR drives Cl − and HCO 3 − secretion and regulates Na + absorption by inhibiting the epithelial Na + channel (ENaC) [ 1 , 16 ]. In case of impaired CFTR function, the combination of decreased Cl − secretion through CFTR and increased Na + resorption through ENaC with consequent water compartmental shifts reduces the amount of ASL decreasing the height of the periciliary fluid and impairing the mucociliary clearance (MCC) [ 1 , 11 , 16 , 17 , 18 , 19 , 20 ]. The lack of HCO 3 − secretion leads to a reduced ASL pH with consequent further Na + and fluid absorption because of the paucity of inhibition of ENaC by the secreted protein short palate lung and nasal epithelial clone 1 (SPLUNC1).…”
Section: The Role Of Cftr Dysfunction In Airway Ep...mentioning
confidence: 99%