Measurement of myo-inositol in diabetic sera by GC/MS/SIM using n-butylboronate derivatives

Hasegawa, M.; Doi, Kunio; Baba, Shigeaki

doi:10.1016/0009-8981(88)90209-4

Cited by 3 publications

(1 citation statement)

References 10 publications

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“…A review paper by Setty and Huang discusses derivatization reactions followed by GC/MS detection, as well as the application of 1 H MRS techniques, for the quantitative measurement of myo -inositol in biological systems . Additional methods for the quantification of myo -inositol in tissue, plasma, serum, and urine samples using derivatization followed by GC/MS analysis in the selected ion monitoring mode can also be found in the literature. , Thin layer or other forms of open column chromatography were also used in early analyses to study myo -inositol in chloroform/methanol extracts from cell homogenates . However, these authors have emphasized careful sample handling due to the autolytic degradative nature of inositides.…”

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Development and Validation of an LC/MS/MS Procedure for the Quantification of Endogenousmyo-Inositol Concentrations in Rat Brain Tissue Homogenates

et al. 2004

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myo-Inositol is being investigated as a biomarker to monitor disease states involving the central nervous system. We have developed and validated a quantitative method to study endogenous myo-inositol metabolism in rat brain tissue. Tissue samples were homogenized, and their myo-inositol content was determined using spiked calibration curves and mass spectrometry. The assay was validated on an LC/MS/MS platform, and specificity was evaluated using accurate mass measurements. A novel chiral LC/MS/MS method was also developed to resolve myo-inositol from other endogenous inositol epimers and confirm the selectivity of the quantitative procedure. The validated method is selective, convenient, precise (<15% RSD), accurate (<15% RE), and sensitive over a linear range of 0.100-100 microg/mL. This method could potentially be used as an instrument for monitoring pathological conditions related to psychotherapeutics, as well as a tool for screening curative pharmaceuticals for efficacy.

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confidence: 99%

Development and Validation of an LC/MS/MS Procedure for the Quantification of Endogenousmyo-Inositol Concentrations in Rat Brain Tissue Homogenates

et al. 2004

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Plasma inositol levels in lithium‐treated manic‐depressives, schizophrenics and controls

Agam

Belfair

Shapiro

et al. 1995

Human Psychopharmacology

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Plasma inositol levels of lithium-treated manic-depressives (LTMD), schizophrenics and healthy volunteers have been studied. In contrast with the findings of Allison and Stewart, that Li treatment raises rat plasma inositol while lowering brain inositol, but in agreement with a previous report of our group of no effect of lithium on CSF inositol levels, we now show no significant difference in plasma inositol concentrations of LTMD as compared with those of control subjects. Plasma inositol levels of the schizophrenics in the present study are slightly but significantly decreased from those of controls.

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Liquid chromatography – mass spectrometry methods for investigating osmolytes and related one-carbon metabolites in health and disease.

McEntyre¹

2016

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Measurement of myo-inositol in diabetic sera by GC/MS/SIM using n-butylboronate derivatives

Cited by 3 publications

References 10 publications

Development and Validation of an LC/MS/MS Procedure for the Quantification of Endogenousmyo-Inositol Concentrations in Rat Brain Tissue Homogenates

Development and Validation of an LC/MS/MS Procedure for the Quantification of Endogenousmyo-Inositol Concentrations in Rat Brain Tissue Homogenates

Plasma inositol levels in lithium‐treated manic‐depressives, schizophrenics and controls

Liquid chromatography – mass spectrometry methods for investigating osmolytes and related one-carbon metabolites in health and disease.

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