Measurement of Myocardial pH by Saturation Transfer in Man

Antioxidant treatment improves in vivo cardiac and skeletal muscle bioenergetics in patients with Friedreich's ataxia

“…Cardiac spectra were acquired using a one‐dimensional chemical shift imaging (1D CSI) sequence to encode signals with depth into the chest 20. Spectral localisation was improved by using presaturation bands positioned over the chest wall using the proton images 21. Cardiac signal was acquired from an 8‐cm‐thick axial slice (again guided by the proton images) and phase‐encoded to provide spectra from 1‐cm‐thick coronal slabs through the heart.…”

“…Typically, composite cardiac 31 P spectra were obtained by adding 2 or 3 1D CSI rows collected from the apex and part of the septum and the posterior wall. Spectra were then fitted using a purpose‐written interactive frequency domain‐fitting program 21. Time domain signals were simulated for PCr, the 3 peaks of ATP, PDE, and 2,3‐DPG incorporating prior knowledge of peak chemical shifts and j‐couplings.…”

Rapid‐sequence phosphorus‐31 magnetic resonance spectroscopy of the human heart using a 1.5‐T clinical system

“…The acquisition time and overall examination time of the two rapid-sequence 31 P-MRS methods that we examined, RS8-4 and RS16-1, were approximately 1/8 and 1/4 those of StdP. 31 P-MRS is a powerful tool for estimating myocardial energy metabolism (2,3,8,15). The myocardial PCr/ATP ratio determined by 31 P-MRS has been used to evaluate patients with cardiac diseases (1-4, 6, 9-12, 14).…”

“…31 P-MRS offers the unique possibility of studying cardiac high-energy phosphate metabolism non-invasively (2,3,8,15). Despite recent studies clearly demonstrating the diagnostic value of 31 P-MRS in cardiac patients (4,6,(9)(10)(11)(12)14), cardiac 31…”

Advances in human cardiac 31P‐MR spectroscopy: SLOOP and clinical applications

“…The contribution of blood to the ATP signals thus can be compensated by measuring the amplitude of the DPG‐signals (16, 24). Nevertheless, blood contamination also impedes the observation of inorganic phosphate ( P i ), and more sophisticated techniques are required if P i or the pH are to be measured (25). The problem of localization is further impeded by the complex motion pattern of the heart.…”