Measurement of neutral ceramidase activity in vitro and in vivo

Simoes, Michael; Saleh, Amalia; Choi, Yong‐Mi; Airola, Michael V.; Haley, John D.; Coant, Nicolas

doi:10.1016/j.ab.2022.114577

Cited by 3 publications

(1 citation statement)

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“…To further elucidate how the reprogrammed cell metabolism process is involved in the inflammatory response of macrophages exposed to PaP-EVs, we analyzed the large nodes (degree > 7) within the core protein group. Consistent with the results obtained from KEGG enrichment analysis, which indicated the activation of inflammation-related pathways, we also observed a significant decrease in the expression of anti-inflammatory factor ASAH1 , and HMOX1 (Figure a). Other large nodes (degree > 7) were annotated in energy metabolism (ATP1B1, CYP27A1) and glucose metabolism pathways (PLCB2, NPL, GALM, GFAT), with most exhibiting a downregulation trend (Figure b).…”

Section: Resultsmentioning

confidence: 99%

Impact of Airborne Pathogen-Derived Extracellular Vesicles on Macrophages Revealed by Raman Spectroscopy and Multiomics

Qin,

Shi,

Zhu

et al. 2023

Environ. Sci. Technol.

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Long-term exposure to the indoor environment may pose threats to human health due to the presence of pathogenic bacteria and their byproducts. Nanoscale extracellular vesicles (EVs) extensively secreted from pathogenic bacteria can traverse biological barriers and affect physio-pathological processes. However, the potential health impact of EVs from indoor dust and the underlying mechanisms remain largely unexplored.Here, Raman spectroscopy combined with multiomics (genomics and proteomics) was used to address these issues. Genomic analysis revealed that Pseudomonas was an efficient producer of EVs that harbored 68 types of virulence factor-encoding genes. Upon exposing macrophages to environmentally relevant doses of Pseudomonas aeruginosa PAO1-derived EVs, macrophage internalization was observed, and release of inflammatory factors was determined by RT-PCR. Subsequent Raman spectroscopy and unsupervised surprisal analysis of EV-affected macrophages distinguished metabolic alterations, particularly in proteins and lipids. Proteomic analysis further revealed differential expression of proteins in inflammatory and metabolism-related pathways, indicating that EV exposure induced macrophage metabolic reprogramming and inflammation. Collectively, our findings revealed that pathogen-derived EVs in the indoor environments can act as a new mediator for pathogens to exert adverse health effects. Our method of Raman integrated with multiomics offers a complementary approach for rapid and in-depth understanding of EVs' impact.

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Section: Resultsmentioning

confidence: 99%