Measurement of Noradrenaline, Dopamine and Serotonin Contents in Follicular Fluid of Human Graafian Follicles after Superovulation Treatment

Bódis, József; Bognár, Zita; Hartmann, G; Török, Á.; If, Csaba

doi:10.1159/000294873

Cited by 38 publications

(33 citation statements)

References 2 publications

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“…The innervation of the ovary by adrenergic and cholinergic nerves has been well documented [5][6][7]. Other studies [8] showed that preovulatory human FF contains noradrenaline, dopamine and serotonin. In an in vitro cell culture system, serotonin exhibited a significant stimulatory effect on PG secretion by human GCs [2].…”

Section: Discussionmentioning

confidence: 92%

Influence of Melatonin on Basal and Gonadotropin-Stimulated Progesterone and Estradiol Secretion of Cultured Human Granulosa Cells and in the Superfused Granulosa Cell System

Bódis

Koppán

Kornya

et al. 2001

Gynecol Obstet Invest

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The aim of this study was to explore the direct action of melatonin (Me) on basal and gonadotropin-stimulated progesterone (PG) and estradiol (E₂) secretion of human granulosa cells (GCs) cultured in serum-free medium and in a superfused GC system. Human GCs were isolated from preovulatory follicular fluid aspirated from 34 women undergoing in vitro fertilization at the University Women’s Hospital of Tübingen. PG and E₂ production was measured in the presence and absence of Me, propranolol, LH or FSH using radioimmunoassay. Statistical analysis of the data was performed by ANOVA and Newman-Keuls test. Me stimulated E₂ secretion in a dose-dependent manner. Propranolol did not cause any change in E₂ secretion, and when given with Me, it only partially blocked but could not entirely prevent E₂ output. There was no statistically significant effect of Me on PG production when Me was administered at concentrations between 10^–4 and 10^–8 M. However, at 10^–3 M Me significantly suppressed PG output of granulosa cells. LH and FSH significantly stimulated the secretion of both steroid hormones. Me significantly reduced LH- and FSH-induced E₂ secretion, as well as LH-stimulated PG output, while it caused only a slight, yet significant decrease in PG secretion. In the superfused GC system, FSH and LH resulted in a significant stimulatory effect on PG release. Me did not modify the stimulatory effect of FSH on PG, while it caused some delay in LH-stimulated PG release. Propranolol and Me had no stimulatory effect on PG release. On the basis of our results we suggest that Me has a direct modulatory effect on basal E₂ and gonadotropin-stimulated E₂ and PG secretion of human GCs. The observed effect may play a physiological role in the regulation of GC function during the menstrual cycle.

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Section: Discussionmentioning

confidence: 92%

Influence of Melatonin on Basal and Gonadotropin-Stimulated Progesterone and Estradiol Secretion of Cultured Human Granulosa Cells and in the Superfused Granulosa Cell System

Bódis

Koppán

Kornya

et al. 2001

Gynecol Obstet Invest

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“…Noradrenaline has been reported to modulate ovarian steroidogenesis [131][132][133][134][135][136][137] instead of stimulating ovulation. These effects of noradrenaline probably are mediated by both a-and b-adrenergic receptors located in the ovary [131][132][133][134]137].…”

Section: Steroidogenesismentioning

confidence: 99%

Hypertension, RAS, and gender: what is the role of aminopeptidases?

Ramírez-Expósito

Martínez-Martos

2008

Heart Fail Rev

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Hypertension is the major risk factor for coronary heart disease, stroke, and renal disease. Also, it is probably the most important risk factor for peripheral vascular disease and vascular dementia. Although hypertension occurs in both men and women, gender differences have been observed. However, whether sex hormones are responsible for the observed gender-associated differences in arterial blood pressure, and which is their mechanism of action, remains unclear. Local and circulating renin-angiotensin systems (RAS) are examples of systems that may be involved in the pathogenesis of hypertension. Classically, angiotensin II (Ang II) has been considered as the effector peptide of the RAS, but Ang II is not the only active peptide. Several of its degradation products, including angiotensin III (Ang III) and angiotensin IV (Ang IV) also possess biological functions. These peptides are formed via the activity of several aminopeptidases. This review will briefly summarize what is known about gender differences in RAS-regulating aminopeptidase activities, their relationship with sex hormones, and their potential role in controlling blood pressure acting through local and circulating RAS.

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“…Tyrosine hydroxylase (TH) expression, a hallmark of dopaminergic and noradrenergic neurons, is found in the human fetal brain before all cortical layers have been formed [4][5][6], suggesting that at this early age DA is involved in proliferation, migration, and differentiation of young neurons [7][8][9]. Ambient DA was detected in follicular fluid of superovulated women [10], and human fetal brain tissue (9-10 gestational weeks [GWs]) in vitro [11]. All together, these findings imply that human neurodifferentiation may be regulated by DA.…”

Section: Introductionmentioning

confidence: 99%

Dopamine Receptors in Human Embryonic Stem Cell Neurodifferentiation

Belinsky

Sirois

Rich

et al. 2013

Stem Cells and Development

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We tested whether dopaminergic drugs can improve the protocol for in vitro differentiation of H9 human embryonic stem cells (hESCs) into dopaminergic neurons. The expression of 5 dopamine (DA) receptor subtypes (mRNA and protein) was analyzed at each protocol stage (1, undifferentiated hESCs; 2, embryoid bodies [EBs]; 3, neuroepithelial rosettes; 4, expanding neuroepithelium; and 5, differentiating neurons) and compared to human fetal brain (gestational week 17-19). D2-like DA receptors (D2, D3, and D4) predominate over the D1-like receptors (D1 and D5) during derivation of neurons from hESCs. D1 was the receptor subtype with the lowest representation in each protocol stage (Stages 1-5). D1/D5-agonist SKF38393 and D2/D3/D4-agonist quinpirole (either alone or combined) evoked Ca 2 + responses, indicating functional receptors in hESCs. To identify when receptor activation causes a striking effect on hESC neurodifferentiation, and what ligands and endpoints are most interesting, we varied the timing, duration, and drug in the culture media. Dopaminergic agonists or antagonists were administered either early (Stages 1-3) or late (Stages 4-5). Early DA exposure resulted in more neuroepithelial colonies, more neuronal clusters, and more TH + clusters. The D1/D5 antagonist SKF83566 had a strong effect on EB morphology and the expression of midbrain markers. Late exposure to DA resulted in a modest increase in TH + neuron clusters (*75%). The increase caused by DA did not occur in the presence of dibutyryl cAMP (dbcAMP), suggesting that DA acts through the cAMP pathway. However, a D2-antagonist (L741) decreased TH + cluster counts. Electrophysiological parameters of the postmitotic neurons were not significantly affected by late DA treatment (Stages 4-5). The mRNA of mature neurons (VGLUT1 and GAD1) and the midbrain markers (GIRK2, LMX1A, and MSX1) were lower in hESCs treated by DA or a D2-antagonist. When hESCs were neurodifferentiated on PA6 stromal cells, DA also increased expression of tyrosine hydroxylase. Although these results are consistent with DA's role in potentiating DA neurodifferentiation, dopaminergic treatments are generally less efficient than dbcAMP alone.

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Measurement of Noradrenaline, Dopamine and Serotonin Contents in Follicular Fluid of Human Graafian Follicles after Superovulation Treatment

Cited by 38 publications

References 2 publications

Influence of Melatonin on Basal and Gonadotropin-Stimulated Progesterone and Estradiol Secretion of Cultured Human Granulosa Cells and in the Superfused Granulosa Cell System

Influence of Melatonin on Basal and Gonadotropin-Stimulated Progesterone and Estradiol Secretion of Cultured Human Granulosa Cells and in the Superfused Granulosa Cell System

Hypertension, RAS, and gender: what is the role of aminopeptidases?

Dopamine Receptors in Human Embryonic Stem Cell Neurodifferentiation

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