Measurement of photosynthetic production of a marine phytoplankton population using a stable 13C isotope

Hama, Takeo; Miyazaki, Tatsuo; Ogawa, Yohsuke; Iwakuma, Toshio; Takahashi, Masayuki; Otsuki, Akira; Ichimura, Shun‐ei

doi:10.1007/bf00396282

Cited by 437 publications

(280 citation statements)

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“…The reproducibility for δ 15 N and δ 13 C measurements were both better than 0.3 ‰. The NF and CF rates were calculated by using similar equations proposed by Montoya et al (1996) and Hama et al (1983), respectively.…”

Section: N 2 and Carbon Fixation Rate Measurementsmentioning

confidence: 99%

Effect of light on N2 fixation and net nitrogen release of Trichodesmium in a field study

et al. 2018

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Abstract. Dinitrogen fixation (NF) by marine cyanobacteria is an important pathway to replenish the oceanic bioavailable nitrogen inventory. Light is the key to modulating NF; however, field studies investigating the light response curve (NF-I curve) of NF rate and the effect of light on diazotrophderived nitrogen (DDN) net release are relatively sparse in the literature, hampering prediction using models. A dissolution method was applied using uncontaminated 15 N 2 gas to examine how the light changes may influence the NF intensity and DDN net release in the oligotrophic ocean. Experiments were conducted at stations with diazotrophs dominated by filamentous cyanobacterium Trichodesmium spp. in the western Pacific and the South China Sea. The effect of light on carbon fixation (CF) was measured in parallel using the 13 C tracer method specifically for a station characterized by Trichodesmium bloom. Both NF-I and CF-I curves showed a I k (light saturation coefficient) range of 193 to 315 µE m −2 s −1 , with light saturation at around 400 µE m −2 s −1 . The proportion of DDN net release ranged from ∼ 6 to ∼ 50 %, suggesting an increasing trend as the light intensity decreased. At the Trichodesmium bloom station, we found that the CF / NF ratio was light-dependent and the ratio started to increase as light was lower than the carbon compensation point of 200 µE m −2 s −1 . Under low-light stress, Trichodesmium physiologically preferred to allocate more energy for CF to alleviate the intensive carbon consumption by respiration; thus, there is a metabolism tradeoff between CF and NF pathways. Results showed that shortterm (< 24 h) light change modulates the physiological state, which subsequently determined the C / N metabolism and DDN net release by Trichodesmium. Reallocation of energy associated with the variation in light intensity would be helpful for prediction of the global biogeochemical cycle of N by models involving Trichodesmium blooms.

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Section: N 2 and Carbon Fixation Rate Measurementsmentioning

confidence: 99%

Effect of light on N2 fixation and net nitrogen release of Trichodesmium in a field study

et al. 2018

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“…Although measuring incorporation of 14 C by the entire phytoplankton community is relatively rapid and inexpensive, the analysis of individual taxa within mixed natural samples is a time-consuming process (isolation and analysis of cells from individual taxa) and is impractical for high-throughput studies. In addition, the radioactivity of this isotope limits its application in the field (Hama et al, 1983). Methods based on 13 C avoid the use of radioactive isotopes; however, analysis can be relatively expensive.…”

Section: Introductionmentioning

confidence: 99%

Snapshot prediction of carbon productivity, carbon and protein content in a Southern Ocean diatom using FTIR spectroscopy

et al. 2015

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Diatoms, an important group of phytoplankton, bloom annually in the Southern Ocean, covering thousands of square kilometers and dominating the region's phytoplankton communities. In their role as the major food source to marine grazers, diatoms supply carbon, nutrients and energy to the Southern Ocean food web. Prevailing environmental conditions influence diatom phenotypic traits (for example, photophysiology, macromolecular composition and morphology), which in turn affect the transfer of energy, carbon and nutrients to grazers and higher trophic levels, as well as oceanic biogeochemical cycles. The paucity of phenotypic data on Southern Ocean phytoplankton limits our understanding of the ecosystem and how it may respond to future environmental change. Here we used a novel approach to create a 'snapshot' of cell phenotype. Using mass spectrometry, we measured nitrogen (a proxy for protein), total carbon and carbon-13 enrichment (carbon productivity), then used this data to build spectroscopy-based predictive models. The models were used to provide phenotypic data for samples from a third sample set. Importantly, this approach enabled the first ever rate determination of carbon productivity from a single time point, circumventing the need for timeseries measurements. This study showed that Chaetoceros simplex was less productive and had lower protein and carbon content during short-term periods of high salinity. Applying this new phenomics approach to natural phytoplankton samples could provide valuable insight into understanding phytoplankton productivity and function in the marine system.

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“…Primary productivity was measured by the simulated in situ methods using a 13 C-technique (Hama et al, 1983). Samples were taken from five depths corresponding to 100, 50, 30, 10 and 1% photon fluxes just above the sea surface, with a submersible pump (Terada ER-25B).…”

Section: Methodsmentioning

confidence: 99%

“…Particulate organic carbon and nitrogen were determined simultaneously. Primary productivity was calculated according to Hama et al (1983). The dark uptake was always corrected for primary productivity.…”

Section: Methodsmentioning

confidence: 99%

“…There have been few measurements of primary productivity since then because the use of 14 C in the natural environment has been strictly restricted in Japan. A stable isotope of carbon, 13 C, has been developed for the determination of primary productivity since the late 1970's (Slawyk et al, 1977;Hama et al, 1983;Kanda et al, 1985a, b;Collos and Slawyk, 1986). However, there are still few data available about primary productivity determined by the 13 C method in Japanese waters (Hama et al, 1983;Kanda et al, 1985b).…”

Section: Introductionmentioning

confidence: 99%

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Primary productivity in a cold water mass and the neighborhood area occurring off Enshu-nada in the late summer of 1989

Shimoto

Matsumura

1992

J Oceanogr

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Primary productivity off Enshu-nada was measured by the 13 C method in September 1989. Primary productivity was estimated in a cold water mass developed off Enshunada for the first time. The obtained value was 469 mgC·m -2 ·d -1 and higher than those in the pelagic area of Kuroshio, but equivalent to those in the neritic and the Oyashio areas. It was indicated that cold water mass is the place where organic matter is produced actively. Extremely high chlorophyll a of more than 5 µg·l -1 were found in the cold water mass. The high productivity was due to high standing crop of phyoplankton. Furthermore, calculated light efficiency and quantum yield showed consistent increase with depth and showed a maximum at 10% light level. Both were larger on the coastal side than those on the oceanic side of the Kuroshio current.

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Measurement of photosynthetic production of a marine phytoplankton population using a stable 13C isotope

Cited by 437 publications

References 17 publications

Effect of light on N<sub>2</sub> fixation and net nitrogen release of <i>Trichodesmium</i> in a field study

Effect of light on N<sub>2</sub> fixation and net nitrogen release of <i>Trichodesmium</i> in a field study

Snapshot prediction of carbon productivity, carbon and protein content in a Southern Ocean diatom using FTIR spectroscopy

Primary productivity in a cold water mass and the neighborhood area occurring off Enshu-nada in the late summer of 1989

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Measurement of photosynthetic production of a marine phytoplankton population using a stable 13C isotope

Cited by 437 publications

References 17 publications

Effect of light on N&lt;sub&gt;2&lt;/sub&gt; fixation and net nitrogen release of &lt;i&gt;Trichodesmium&lt;/i&gt; in a field study

Effect of light on N&lt;sub&gt;2&lt;/sub&gt; fixation and net nitrogen release of &lt;i&gt;Trichodesmium&lt;/i&gt; in a field study

Snapshot prediction of carbon productivity, carbon and protein content in a Southern Ocean diatom using FTIR spectroscopy

Primary productivity in a cold water mass and the neighborhood area occurring off Enshu-nada in the late summer of 1989

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Effect of light on N<sub>2</sub> fixation and net nitrogen release of <i>Trichodesmium</i> in a field study

Effect of light on N<sub>2</sub> fixation and net nitrogen release of <i>Trichodesmium</i> in a field study