2009
DOI: 10.2460/ajvr.70.11.1315
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Measurement of plasma renin concentration in cats by use of a fluorescence resonance energy transfer peptide substrate of renin

Abstract: The study data indicated that the assay involving the FRET peptide substrate of renin is potentially a rapid and specific method for measurement of plasma renin concentration in cats.

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Cited by 9 publications
(6 citation statements)
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“…Renin enzymatic activity from EDTA-aprotinin supplemented mouse plasma samples (described in the Section 4.2) were measured in a 96-well microplate (Synergy HT reader and Gen5 v1.09 software, BioTek Instruments, Inc., Winooski, VT, USA) and quantified using exogenous fluorescence resonance transfer (FRET) peptide substrates of renin FRET-QXL™520/5-FAM, optimized for mouse renin (SensoLyte 520 mouse renin assay kit, AnaSpec, Fremont, CA, USA) as previously reported [25,27,32,78,79,80,81]. Cleavage of the FRET substrate by mouse renin results in the recovery of quenched fluorescence of 5-FAM, which was detected at excitation/emission = 490/520 nm with minimum autofluorescence of plasma samples.…”
Section: Methodsmentioning
confidence: 99%
“…Renin enzymatic activity from EDTA-aprotinin supplemented mouse plasma samples (described in the Section 4.2) were measured in a 96-well microplate (Synergy HT reader and Gen5 v1.09 software, BioTek Instruments, Inc., Winooski, VT, USA) and quantified using exogenous fluorescence resonance transfer (FRET) peptide substrates of renin FRET-QXL™520/5-FAM, optimized for mouse renin (SensoLyte 520 mouse renin assay kit, AnaSpec, Fremont, CA, USA) as previously reported [25,27,32,78,79,80,81]. Cleavage of the FRET substrate by mouse renin results in the recovery of quenched fluorescence of 5-FAM, which was detected at excitation/emission = 490/520 nm with minimum autofluorescence of plasma samples.…”
Section: Methodsmentioning
confidence: 99%
“…Within 30 min of collection, EDTA-plasma samples were prepared by centrifugation, frozen, and stored at Ϫ20°C until analysis. Plasma renin concentrations were determined using a modification of a commercial fluorometric kit (SensoLyte 520 Rat Renin Assay Kit, AnaSpec, Fremont, CA) (19). Briefly, stock rat renin substrate and recombinant rat renin were diluted at 1:50 and 1:500 with assay buffer, respectively.…”
Section: Methodsmentioning
confidence: 99%
“…Blood was collected in EDTA (7.2 mg, Vacutainer, BD, Franklin Lakes, NJ) via the arterial catheter. Plasma renin concentrations were measured in triplicate via a fluorometric kit (SensoLyte 520 Rat Renin Assay Kit, AnaSpec, Inc., Fremont, CA), as described previously [23, 24]. …”
Section: Methodsmentioning
confidence: 99%