Measurement of Platelet Microparticles

Zwicker, Jeffrey I.; Lacroix, Romaric; Dignat-George, Françoise; Furie, Barbara C.; Furie, Bruce

doi:10.1007/978-1-61779-307-3_10

Cited by 20 publications

(20 citation statements)

References 53 publications

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“…The flow cytometric analysis of platelets is being used increasingly for the detection of agonist-induced alterations in the membrane expression of platelet activation markers [1,2], as well as for the detection and enumeration of platelet-derived microparticles [3,4]. Flow cytometry may be used to detect an increased surface expression of P-selectin (CD62p) or LIMP (CD63), or activation of the platelet glycoprotein IIb/IIIa complex using a PAC-1 antibody.…”

Section: Accepted Manuscriptmentioning

confidence: 99%

Flow cytometry demonstrates differences in platelet reactivity and microparticle formation in subjects with thrombocytopenia or thrombocytosis due to primary haematological disorders

Connor¹,

D.F.²,

Joseph³

2013

Thrombosis Research

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Section: Accepted Manuscriptmentioning

confidence: 99%

Flow cytometry demonstrates differences in platelet reactivity and microparticle formation in subjects with thrombocytopenia or thrombocytosis due to primary haematological disorders

Connor¹,

D.F.²,

Joseph³

2013

Thrombosis Research

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“…Microparticles were analyzed as previously described. 20,21 The presence of uPA, uPAR and tissue plasminogen activator (tPA) was evaluated by flow cytometry using FITC-labeled antibodies on microparticles gated according to positivity for CD146-PE (endothelial microparticles), CD59-PE (endothelial, erythrocyte and leukocyte microparticles) or CD41-PE (platelet microparticles) (Online Supplementary Figure S3). Antibodies matched for protein concentration and fluorescence/protein ratio were used as controls.…”

Section: Characterization Of Microparticles By Flow Cytometry and Enzmentioning

confidence: 99%

Leukocyte- and endothelial-derived microparticles: a circulating source for fibrinolysis

et al. 2012

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The online version of this article has a Supplementary Appendix. BackgroundWe recently assigned a new fibrinolytic function to cell-derived microparticles in vitro. In this study we explored the relevance of this novel property of microparticles to the in vivo situation. Design and MethodsCirculating microparticles were isolated from the plasma of patients with thrombotic thrombocytopenic purpura or cardiovascular disease and from healthy subjects. Microparticles were also obtained from purified human blood cell subpopulations. The plasminogen activators on microparticles were identified by flow cytometry and enzyme-linked immunosorbent assays; their capacity to generate plasmin was quantified with a chromogenic assay and their fibrinolytic activity was determined by zymography. ResultsCirculating microparticles isolated from patients generate a range of plasmin activity at their surface. This property was related to a variable content of urokinase-type plasminogen activator and/or tissue plasminogen activator. Using distinct microparticle subpopulations, we demonstrated that plasmin is generated on endothelial and leukocyte microparticles, but not on microparticles of platelet or erythrocyte origin. Leukocyte-derived microparticles bear urokinase-type plasminogen activator and its receptor whereas endothelial microparticles carry tissue plasminogen activator and tissue plasminogen activator/inhibitor complexes. ConclusionsEndothelial and leukocyte microparticles, bearing respectively tissue plasminogen activator or urokinase-type plasminogen activator, support a part of the fibrinolytic activity in the circulation which is modulated in pathological settings. Awareness of this blood-borne fibrinolytic activity conveyed by microparticles provides a more comprehensive view of the role of microparticles in the hemostatic equilibrium.Key words: fibrinolytic microparticles, plasmin, plasminogen, uPA; tPA. Plawinski L, Robert S, Doeuvre L, Sabatier F, Martinez de Lizarrondo S, Mezzapesa A, Anfosso F, Leroyer AS, Poullin P, Jourde N, Njock M-S, Boulanger CM, Anglés-Cano E, and Dignat-George F. Leukocyte-and endothelial-derived microparticles: a circulating source for fibrinolysis. Haematologica 2012;97(12):1864-1872. doi:10.3324/haematol.2012 This is an open-access paper. Citation: Lacroix R, Leukocyte-and endothelial-derived microparticles: a circulating source for fibrinolysis ABSTRACT© F e r r a t a S t o r t i F o u n d a t i o n

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“…This technology is designed to scan and sort at a rate of thousands of single cells or particles per second (van der Pol et al, 2010). Flow cytometry is widely used to detect origin, size, and morphology of circulating ECVs (Kim et al, 2002; Hunter et al, 2008; Kesimer et al, 2009; Mobarrez et al, 2010; Orozco and Lewis, 2010; Zwicker et al, 2012). Through hydrodynamic focusing, the suspended cells flow through a compressed chamber to the interrogation point, where the sample encounters the laser.…”

Section: Characterization Of Extracellular Vesiclesmentioning

confidence: 99%

Alternative Methods for Characterization of Extracellular Vesicles

Momen-Heravi¹,

Balaj²,

Alian³

et al. 2012

Front. Physio.

126

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Extracellular vesicles (ECVs) are nano-sized vesicles released by all cells in vitro as well as in vivo. Their role has been implicated mainly in cell–cell communication, but also in disease biomarkers and more recently in gene delivery. They represent a snapshot of the cell status at the moment of release and carry bioreactive macromolecules such as nucleic acids, proteins, and lipids. A major limitation in this emerging new field is the availability/awareness of techniques to isolate and properly characterize ECVs. The lack of gold standards makes comparing different studies very difficult and may potentially hinder some ECVs-specific evidence. Characterization of ECVs has also recently seen many advances with the use of Nanoparticle Tracking Analysis, flow cytometry, cryo-electron microscopy instruments, and proteomic technologies. In this review, we discuss the latest developments in translational technologies involving characterization methods including the facts in their support and the challenges they face.

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Measurement of Platelet Microparticles

Cited by 20 publications

References 53 publications

Flow cytometry demonstrates differences in platelet reactivity and microparticle formation in subjects with thrombocytopenia or thrombocytosis due to primary haematological disorders

Flow cytometry demonstrates differences in platelet reactivity and microparticle formation in subjects with thrombocytopenia or thrombocytosis due to primary haematological disorders

Leukocyte- and endothelial-derived microparticles: a circulating source for fibrinolysis

Alternative Methods for Characterization of Extracellular Vesicles

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