Measurement of proliferating cell nuclear antigen (PCNA) and its clinical application in oral cancers

Tsuji, Tatsuo; Sasaki, Kohsuke; Kimura, Yasutoshi; Yamada, Kazuto; Mori, Masahiko; Shinozaki, Fumihiko

doi:10.1016/s0901-5027(05)80765-9

Cited by 51 publications

(37 citation statements)

References 7 publications

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“…PCNA is one of the proliferation proteins and the antibodies to this protein would help to assess the tumour proliferation activity of oral lesions [8].…”

Section: Discussionmentioning

confidence: 99%

Proliferating Cell Nuclear Antigen in Premalignancy and Oral Squamous Cell Carcinoma

Poosarla

Ramesh

Gudiseva

et al. 2015

JCDR

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“…PCNA is one of the proliferation proteins and the antibodies to this protein would help to assess the tumour proliferation activity of oral lesions [8].…”

Section: Discussionmentioning

confidence: 99%

Proliferating Cell Nuclear Antigen in Premalignancy and Oral Squamous Cell Carcinoma

Poosarla

Ramesh

Gudiseva

et al. 2015

JCDR

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“…Immunostaining for proliferating cell nuclear antigen (PCNA) and Ki67 of the olfactory epithelium allowed to identify the most phases of the cell cycle as was described by Tsuji et al (1992) and Pendleton et al (1993) studying expression of protein markers in human.…”

Section: Pcna and Ki67 Immunostaining Of Cells From Oe Of 60-day Agedmentioning

confidence: 99%

Establishment of a protocol for obtention of neuronal stem cells lineages from the dog olfactory epithelium

et al. 2010

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Miglino M.A. 2010. Establishment of a protocol for obtention of neuronal stem cells lineages from the dog olfactory epithelium. Pesquisa . Departamento de Ciência Animal, Campus Universitário, Bairro Cibrazen, Bom Jesus, PI 64900-000, Brazil. E-mail: flavioribeiro@ufpi.edu.br A morphological and cell culture study from nasal mucosa of dogs was performed in order to establish a protocol to obtain a cell population committed to neuronal lineage, as a proposal for the treatment of traumatic and degenerative lesions in these animals, so that in the future these results could be applied to the human species. Twelve mongrel dogs of 60-day aged pregnancy were collected from urban pound dogs in São Paulo. Tissue from cribriform ethmoidal lamina of the fetuses was collected at necropsy under sterile conditions around 1h to 2h postmortem by uterine sections and sections from the fetal regions described above. Isolated cells of this tissue were added in DMEM/F-12 medium under standard conditions of incubation (5% CO 2 , >37°C). Cell culture based on isolated cells from biopsies of the olfactory epithelium showed rapid growth when cultured for 24 hours, showing phase-bright sphere cells found floating around the fragments, attached on culture flasks. After 20 days, a specific type of cells, predominantly ellipsoids or fusiform cells was characterized in vitro. The indirect immunofluorescence examination showed cells expressing markers of neuronal precursors (GFAP, neurofilament, oligodendrocyte, and III â-tubulin). The cell proliferation index showed Ki67 immunostaining with a trend to label cell groups throughout the apical region, while PCNA immunostaining label predominantly cell groups lying above the basal lamina. The transmission electron microscopy from the olfactory epithelium of dogs revealed cells with electron-dense cytoplasm and preserving the same distribution as those of positive cell staining for PCNA. Metabolic activity was confirmed by presence of euchromatin in the greatest part of cells. All these aspects give subsidies to support the hypothesis about resident progenitor cells among the basal cells of the olfactory epithelium, committed to renewal of these cell populations, especially neurons. RESUMO.-[Estabelecimento de um protocolo para obtenção de linhagens de células-tronco neuronais a partir do epitélio olfatório de cães.] Foi realizado um estudo morfológico e por cultivo celular a partir de células provenientes da mucosa olfatória de cães, como forma de estabelecer um protocolo de cultivo, como uma proposta para o tratamento de lesões traumáticas e nervosas degenerativas nestes animais e futuramente, para que tais resultados possam ser aplicados a espécie humana. Foram utilizados doze cães sem raça definida, a termo, oriundos de castrações do Centro de Controle de Zoonoses de São Paulo. O tecido da lâmina cribiforme do etmóide dos fetos foi coletado sob necropsia, em condições estéreis, 1 a 2 horas post mortem, por meio de incisão uterina e acesso da região fetal supracitada. Depois as células isol...

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“…Cell kinetic data may also be a useful adjunct to histologically based tumor classifications, and are among the most important indicators of treatment response and relapse in many types of cancers. 5,6 In justification of the reasons above, many biologic markers have been proposed to provide information on the differentiation, proliferation, and prognosis of various lesions. Monoclonal antibodies directed toward nuclear antigens are increasingly used as tools to obtain valuable information concerning the proliferative changes of the tumors.…”

Section: Introductionmentioning

confidence: 99%

Proliferative Index in Invasive Tumor Front of Oral Squamous Cell Carcinoma: A Potential Prognostic Indicator

Bhuyan¹,

Sarangi²,

Das³

et al. 2018

The Journal of Contemporary Dental Practice

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Aim:The aim of the study was to evaluate the proliferative index (PI) at their invasive front of oral cancer and their association with Bryne's grades of oral squamous cell carcinoma (OSCC) and compare the PI with lymph node metastasis, site of involvement, and habits. Materials and methods:The Ki-67 antigen expression was immunohistochemically evaluated in a total of 102 cases that included the histopathologically diagnosed archival specimens of OSCCs. They were subdivided by Bryne's histopathological grading into grade I (40 cases), grade II (32 cases), and grade III (30 cases). The nucleus with brown stain was considered positive. Cells were counted under 400× magnification. The proliferative activity thus determined was then expressed as a percentage of Ki-67 labeling index (Ki-67 LI) positive cells. Results:A stepwise increase in the mean Ki-67 LI was found from grade I to III squamous cell carcinoma, thus correlating with the histological grading. In addition, there was a higher PI seen in cases associated with metastatic lymph node, which concords with the higher biologic aggressiveness and poor prognosis of the lesion. Conclusion:The present study shows a definitive correlation of Ki-67 antigen with the Bryne's histological grading, all the parameters of Bryne's grading for OSCC and lymph node status of the patient proving its association as an effective tool to grade the tumors and finally read the prognosis of the tumor. Clinical significance: Cell proliferation is regarded as one of the most important biologic mechanisms in oncogenesis. The role of cell proliferation in tumor progression has been inferred in studies concerned with human cancer by comparing the PI of normal tissue, preneoplastic and neoplastic lesions. The Ki-67 antigen-labeled cells can prove to be an effective aid to grade the tumors. It might be possible to standardize and objectify tumor grading among pathology laboratories. Proliferative Index in Invasive Tumor

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Measurement of proliferating cell nuclear antigen (PCNA) and its clinical application in oral cancers

Cited by 51 publications

References 7 publications

Proliferating Cell Nuclear Antigen in Premalignancy and Oral Squamous Cell Carcinoma

Proliferating Cell Nuclear Antigen in Premalignancy and Oral Squamous Cell Carcinoma

Establishment of a protocol for obtention of neuronal stem cells lineages from the dog olfactory epithelium

Proliferative Index in Invasive Tumor Front of Oral Squamous Cell Carcinoma: A Potential Prognostic Indicator

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