Measurement of reticulated platelets by simple flow cytometry: An indirect thrombocytopoietic marker

Jiménez, M. Monteagudo; Guedán, M.J. Amengual; Martin, L.; Campos, J.A. Soler; Martínez, I. Roig; Vilella, Carles Tolosa

doi:10.1016/j.ejim.2006.03.006

Cited by 35 publications

(17 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…First described in 1969 ( 70 ), reticulated platelets are immature platelets circulating in the blood, containing a residual RNA. They can be analyzed with flow cytometry and give an indirect determination of thrombopoietic rate ( 71 ). Lack of standardization of methods and definition of threshold values make difficult the interpretation of RPLT value ( 72 ): even if the applicability of RPLT determination is not completely defined, recent works conclude that this is a promising tool to distinguish thrombocytopenia caused by bone marrow hypoproduction to that one due to platelet destruction ( 73 ).…”

Section: Laboratory Investigationsmentioning

confidence: 99%

The Centenary of Immune Thrombocytopenia—Part 2: Revising Diagnostic and Therapeutic Approach

2017

View full text Add to dashboard Cite

Primary immune thrombocytopenia (ITP) is the most common cause of thrombocytopenia in children and adolescents and can be considered as a paradigmatic model of autoimmune disease. This second part of our review describes the clinical presentation of ITP, the diagnostic approach and overviews the current therapeutic strategies. Interestingly, it suggests an algorithm useful for differential diagnosis, a crucial process to exclude secondary forms of immune thrombocytopenia (IT) and non-immune thrombocytopenia (non-IT), which require a different therapeutic management. Advances in understanding the pathogenesis led to new therapeutic targets, as thrombopoietin receptor agonists, whose role in treatment of ITP will be discussed in this work.

show abstract

Section: Laboratory Investigationsmentioning

confidence: 99%

The Centenary of Immune Thrombocytopenia—Part 2: Revising Diagnostic and Therapeutic Approach

2017

View full text Add to dashboard Cite

show abstract

“…"Flou"-citometrijska analiza retikulovanih trombocita je zato i našla svoje mesto kao dodatna, neinvazivna dijagnostička metoda u ispitivanju trombocitopenija. Brojne studije su u poslednjih dvadesetak godina prikazale retikulovane trombocite u perifernoj krvi kao odraz stepena megakariopoeze u kostnoj srži [22]. Zato je i zadatak biohemijskih laboratorija da podstaknu ovakvu vrstu ispitivanja, u cilju dobijanja kompletnijih informacija o dijagnozi, tretmanu i prognozi trombocitopenija.…”

Section: Zaključakunclassified

Application of new parameters of platelets (CD61 and reticulated platelets) in laboratory monitoring trombocytopenia

Krsmanović¹,

Stanković²

2018

NČ urgent medic Halo 194

View full text Add to dashboard Cite

“…In 1969, Ingram and Coopersmith were the first to show an increase in immature platelets in mature beagles subjected to severe blood loss, reflecting increased thrombopoietic activity (5). Several more recent publications confirmed this observation (2,(10)(11)(12). This might also be of interest in patients undergoing stem cell transplantation (SCT) for hematological malignancies (7,(13)(14)(15)(16)(17)(18)(19).…”

mentioning

confidence: 96%

Immature platelet fraction measured on the Sysmex XN hemocytometer predicts thrombopoietic recovery after autologous stem cell transplantation

Linden

Klinkenberg

Meex

et al. 2014

European J of Haematology

View full text Add to dashboard Cite

ObjectivesA period of thrombocytopenia is common after stem cell transplantation (SCT). To prevent serious bleeding complications, prophylactic platelet transfusions are administered. Previous studies have shown that a rise in immature platelets precedes recovery of platelet count. Our aim was to define a cutoff value for immature platelets predicting thrombopoietic recovery within 2 d.MethodsHematological parameters were measured on the Sysmex XN hemocytometer. We calculated reference change values (RCV) for platelets in eight healthy individuals as marker for platelet recovery. To define a cutoff value, we performed ROC analysis using data from 16 autologous SCT patients.ResultsRCV for platelet concentration was 14.1%. Platelet recovery was observed 13 (median; range 9–31) days after SCT. Increase in immature platelet fraction (IPF) before platelet recovery was seen in all autologous SCT patients. Optimal cutoff IPF was found to be 5.3% for platelet recovery within 2 d (specificity 0.98, sensitivity 0.47, positive predictive value 0.93).ConclusionsWe identified an optimal cutoff value for IPF 5.3% to predict platelet recovery after autologous SCT within 2 d. Implementing this cutoff value in transfusion strategy may reduce the number of prophylactic platelet transfusions.

show abstract

Measurement of reticulated platelets by simple flow cytometry: An indirect thrombocytopoietic marker

Cited by 35 publications

References 11 publications

The Centenary of Immune Thrombocytopenia—Part 2: Revising Diagnostic and Therapeutic Approach

The Centenary of Immune Thrombocytopenia—Part 2: Revising Diagnostic and Therapeutic Approach

Application of new parameters of platelets (CD61 and reticulated platelets) in laboratory monitoring trombocytopenia

Immature platelet fraction measured on the Sysmex XN hemocytometer predicts thrombopoietic recovery after autologous stem cell transplantation

Contact Info

Product

Resources

About