Measurement of ribavirin and evaluation of its stability in human plasma by high-performance liquid chromatography with UV detection

Loregian, Arianna; Scarpa, Maria Cristina; Pagni, Silvana; Parisi, Saverio Giuseppe; Palù, Giorgio

doi:10.1016/j.jchromb.2007.05.039

Cited by 37 publications

(22 citation statements)

References 25 publications

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“…DCV and RBV concentrations were quantified with validated high-performance liquid chromatography methods with ultraviolet detection (unpublished results) in plasma collected before taking drugs (Ctrough). [2][3] The study was notified to the Ethical Committee for Clinical Experimentation, Padova Province (27602/16).…”

Section: Methodsmentioning

confidence: 99%

Daclatasvir plasma level and resistance selection in HIV patients with hepatitis C virus cirrhosis treated with daclatasvir, sofosbuvir, and ribavirin

Parisi

Loregian

Andreis

et al. 2016

International Journal of Infectious Diseases

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Section: Methodsmentioning

confidence: 99%

Daclatasvir plasma level and resistance selection in HIV patients with hepatitis C virus cirrhosis treated with daclatasvir, sofosbuvir, and ribavirin

Parisi

Loregian

Andreis

et al. 2016

International Journal of Infectious Diseases

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“…The extraction process and chromatography produced accuracy and precision data consistent with FDA guidelines [10]. The lower limit of quantification used for this assay was 0.05 μg/mL and is identical to that used for current plasma assays developed for RBV quantification [16]. Correlation between RBV DBS and RBV plasma concentrations supports the use of DBS for pharmacokinetic studies in the future.…”

Section: Discussionmentioning

confidence: 58%

Development and validation of a dried blood spot assay for the quantification of ribavirin using liquid chromatography coupled to mass spectrometry

Jimmerson

Zheng

Bushman

et al. 2014

Journal of Chromatography B

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Efficient, inexpensive and sensitive assays for the measurement of drugs are of interest for pharmacokinetic and pharmacodynamics (PK-PD) analysis. Dried blood spots (DBS) are a unique bioanaltyical matrix with the potential to fulfill this interest for the measurement of numerous analytes. Here we describe the development and validation of a reversed-phase high performance liquid chromatographic (LC), tandem mass spectrometry (MS/MS) assay for the determination of ribavirin (RBV) in DBS. A 3mm punch from spotted and dried whole blood was extracted in methanol utilizing isotopically labeled internal standard for LC-MS/MS analysis. Validation was performed over a range of 0.05 μg/mL to 10.0 μg/mL and the method was shown to be precise (coefficient of variation ≤ 15%) and accurate (within ±15% of control). These acceptance criteria were met for hematocrit ranges of 20-54%, for center versus edge punches and for spot volumes from 10-60 μL. RBV was stable for up to 140 days at room temperature and −20°C as well as for three freeze/thaw cycles. Correlation of RBV in DBS versus in plasma yielded r2 ≥ 0.98 demonstrating that DBS can be used as an alternative to plasma for PK-PD studies in human subjects.

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“…Limited work was available for DAPD and DXG. However, ZDV quantification by LC-MS/MS, using reverse phase chromatography, has been described previously [19,22–25]. Short runs were used for ZDV detection with mobile phase containing 0.1% of acetic acid [25] or at neutral pH [19,24].…”

Section: Discussionmentioning

confidence: 99%

Simultaneous quantification of 9-(β-d-1,3-dioxolan-4-yl)guanine, Amdoxovir and Zidovudine in human plasma by liquid chromatography–tandem mass spectrometric assay

Fromentin¹,

Asif²,

Obikhod³

et al. 2009

Journal of Chromatography B

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A sensitive method was developed and validated for simultaneous measurement of an investigational antiviral nucleoside, Amdoxovir (DAPD), its deaminated metabolite 9-(β-D-1,3-dioxolan-4-yl) guanine (DXG), and Zidovudine (ZDV) in human plasma. This method employed high-performance liquid chromatography-tandem mass spectrometry with electrospray ionization. DXG and DAPD separation with sufficient resolution was necessary since they differ in only one mass to charge ratio, which increases the risk of overlapping MS/MS signals. However, the new method was observed to have functional sensitivity and specificity without interference. Samples were purified by ultrafiltration after protein precipitation with methanol. The total run time was 29 min. A linear calibration range from 2 to 3,000 ng mL −1 and 2 to 5,000 ng mL −1 was achieved for DAPD and DXG, and ZDV, respectively. Precisions and accuracies were both ± 15% (± 20% for the lower limit of quantification) and recoveries were higher than 90%. Matrix effects/ion suppressions were also investigated. The analytes were chemically stable under all relevant conditions and the method was successfully applied for the analysis of plasma samples from HIV-infected persons treated with combinations of DAPD and ZDV.

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Measurement of ribavirin and evaluation of its stability in human plasma by high-performance liquid chromatography with UV detection

Cited by 37 publications

References 25 publications

Daclatasvir plasma level and resistance selection in HIV patients with hepatitis C virus cirrhosis treated with daclatasvir, sofosbuvir, and ribavirin

Daclatasvir plasma level and resistance selection in HIV patients with hepatitis C virus cirrhosis treated with daclatasvir, sofosbuvir, and ribavirin

Development and validation of a dried blood spot assay for the quantification of ribavirin using liquid chromatography coupled to mass spectrometry

Simultaneous quantification of 9-(β-d-1,3-dioxolan-4-yl)guanine, Amdoxovir and Zidovudine in human plasma by liquid chromatography–tandem mass spectrometric assay

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