Measurement of Selenium in Clinical Specimens

Sheehan, T. M. T.; Halls, David J.

doi:10.1177/000456329903600302

Cited by 37 publications

(25 citation statements)

References 85 publications

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“…Blood selenium concentration is generally considered a useful measure of both selenium status and intake, but other tissues such as hair and nails are also used (Nève, 1991;Burk & Levander, 1999;Sheehan & Halls, 1999). Plasma or serum selenium reflects short-term status, and erythrocyte selenium reflects longer-term status, due to the incorporation of selenium during synthesis of these cells.…”

Section: Biochemical Tests For Nutritional Status Of Seleniummentioning

confidence: 99%

Assessment of requirements for selenium and adequacy of selenium status: a review

2004

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Objective: The intent of this review is to evaluate the scientific evidence for the assessment of adequacy of selenium status and of the requirements for selenium. From this evidence, attempts have been made to define levels of plasma selenium and dietary selenium intake, which could be used for the assessment of deficiency or adequacy of selenium status. Method: The first section briefly reviews the methods for assessment of selenium status. The second section outlines the requirements for selenium based on a number of criteria, and how these have been translated into recommended intakes of selenium. In the final section, levels of plasma selenium and dietary intake based on different criteria of adequacy have been proposed. Results and conclusion: The minimum requirement for selenium is that which prevents the deficiency disease, Keshan disease. The recommended intakes of selenium have been calculated from the requirement for optimum plasma glutathione peroxidase (GPx) activity that must, because of the hierarchy of selenoproteins, also take account of the amounts needed for normal levels of other biologically necessary selenium compounds. Whether optimal health depends upon maximization of GPx or other selenoproteins, however, has yet to be resolved, and the consequences of less-than-maximal GPx activities or mRNA levels need investigation. Intakes, higher than recommended intakes, and plasma selenium concentrations that might be protective for cancer or result in other additional health benefits have been proposed. There is an urgent need for more large-scale trials to assess any such beneficial effects and to provide further data on which to base more reliable estimates for intakes and plasma selenium levels that are protective.

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Section: Biochemical Tests For Nutritional Status Of Seleniummentioning

confidence: 99%

Assessment of requirements for selenium and adequacy of selenium status: a review

2004

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“…Serum Selenium levels were determined by graphite furnace atomic absorption spectroscopy (GFAAS) (Perkin Elmer Atomic Absorption Spectrophotometer A Analyst 800), using an Electrodeless Discharge Lamp (EDL) at 196.0 nm, with transversal heated furnace atomizer (THGA) and Zeeman effect background correction as described elsewhere (Sheehan and Halls, 1999). The interassay variation was 3.1%.…”

Section: Analytical Proceduresmentioning

confidence: 99%

Maternal – neonatal serum selenium and copper levels in Greeks and Albanians

Schulpis

Karakonstantakis

Gavrili³

et al. 2004

Eur J Clin Nutr

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Aim: To evaluate selenium (Se) and copper (Cu) concentrations in Greek and Albanian immigrant mothers and in the cord blood of their newborns. Subjects and methods: From 1118 Greek and 820 Albanian mothers and from the cord blood of their neonates blood was obtained for Se and Cu measurement. Se and Cu concentrations were determined in sera with graphite furnace atomic absorption spectroscopy (GFAAAS) and atomic absorption spectrometry, respectively. In all, 30 days' nutrient intakes were evaluated in both groups. Results: Animal protein, Se and Cu intakes were poor in the Albanians vs the Greeks (Po0.001). Se concentrations in the Greek mothers (68.378.5 mg/l) and in their newborns (37.0278.9 mg/l) were found higher as compared with those in Albanian mothers (37.479.9 mg/l) and in their newborns (34.379.1 mg/l) (Po0.001). Cu levels were also found higher (Po0.001) in the Greek mothers (16877353 mg/l) and in their neonates (449787 mg/l) compared with those in the Albanian mothers (9597318 mg/l) and in their newborns (229767 mg/l). Additionally, 31.5% of neonates born to Albanian women with Se concentrations less than 28 mg/l had higher Se levels (Po0.01) than their mothers. Conclusions: The low Se and Cu levels evaluated in the Albanian mothers and their newborns could be related to their poor animal protein intake which could be the consequence of their low socioeconomic status. As an effective preventive measure, accurate dietetic strategies to assess the requirements of pregnant immigrant women for trace elements may be planned in Greece.

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“…From the data presented in Table 3, it became evident that some proteins, such as those corresponding to Spots 10,13,15,16,19 and 20, were more abundant when diets were supplemented with SeMSeCys. Since Spots 10,11,13,14,15,17,18,19 and 20 were not detected in the control conditions, P values could not be calculated, although we considered that these proteins were overexpressed in response to SeMSeCys supplementation.…”

Section: Resultsmentioning

confidence: 99%

“…Alternative measurements of selenium bioavailability that are based on the assessment of the activity of other selenoproteins have been proposed [16,17]. However, their use as selenium biomarkers has not been demonstrated quite likely because of analytical requirements and low reproducibility.…”

Section: Nutritional Biomarkers and Selenium Intakementioning

confidence: 99%

Dietary supplementation with selenomethylselenocysteine produces a differential proteomic response

Mahn

Toledo

Ruz

2009

The Journal of Nutritional Biochemistry

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Measurement of Selenium in Clinical Specimens

Abstract: Additional key phrases: molecular fluorescence spectrometry; hydride; generation atomic absorption lind atomic fluorescence spectrometry; graphite furnace atomic absorption spectrometry; inductively-coupled plasma mass spectrometry

Cited by 37 publications

References 85 publications

Assessment of requirements for selenium and adequacy of selenium status: a review

Assessment of requirements for selenium and adequacy of selenium status: a review

Maternal – neonatal serum selenium and copper levels in Greeks and Albanians

Dietary supplementation with selenomethylselenocysteine produces a differential proteomic response

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