Measurement of serum alkaline phosphatase

Davidson, D F

doi:10.1016/0141-0229(79)90004-8

Cited by 6 publications

(5 citation statements)

References 74 publications

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“…The mean plasma alkaline phosphatase activities in the mice from both sexes of the three strains from the metabolic cage study were lower than that from the group-housed mice, and this is likely attributed to the different assays used to assess this biochemical parameter, as previously reported for the activity of this enzyme. 17 A previous study of fasted, group-housed adult mice at the ages of 3, 6 and 12 months has reported that age does not have significant effects on the majority of electrolyte and metabolite concentrations, 13 with the exception of plasma urea concentrations, which declined with age in B6J mice, and alkaline phosphatase activity, which was elevated in the youngest group in all three strains. Our findings in the 24-to 30-week-old mice housed in metabolic cages are similar to those of these mice at three different ages, and these combined results suggest that the age of the adult animal is not a significant contributor in determining the concentrations of plasma electrolytes and metabolites that were measured in these studies ( Table 4).…”

Section: Discussionmentioning

confidence: 92%

Establishing normal plasma and 24-hour urinary biochemistry ranges in C3H, BALB/c and C57BL/6J mice following acclimatization in metabolic cages

et al. 2010

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Physiological studies of mice are facilitated by normal plasma and 24-hour urinary reference ranges, but variability of these parameters may increase due to stress that is induced by housing in metabolic cages. We assessed daily weight, food and water intake, urine volume and final day measurements of the following: plasma sodium, potassium, chloride, urea, creatinine, calcium, phosphate, alkaline phosphatase, albumin, cholesterol and glucose; and urinary sodium, potassium, calcium, phosphate, glucose and protein in 24- to 30-week-old C3H/HeH, BALB/cAnNCrl and C57BL/6J mice. Between 15 and 20 mice of each sex from all three strains were individually housed in metabolic cages with ad libitum feeding for up to seven days. Acclimatization was evaluated using general linear modelling for repeated measures and comparison of biochemical data was by unpaired t-test and analysis of variance (SPSS version 12.0.1). Following an initial 5-10% fall in body weight, daily dietary intake, urinary output and weight in all three strains reached stable values after 3-4 days of confinement. Significant differences in plasma glucose, cholesterol, urea, chloride, calcium and albumin, and urinary glucose, sodium, phosphate, calcium and protein were observed between strains and genders. Thus, these results provide normal reference values for plasma and urinary biochemistry in three strains housed in metabolic cages and demonstrate that 3-4 days are required to reach equilibrium in metabolic cage studies. These variations due to strain and gender have significant implications for selecting the appropriate strain upon which to breed genetically-altered models of metabolic and renal disease.

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Section: Discussionmentioning

confidence: 92%

Establishing normal plasma and 24-hour urinary biochemistry ranges in C3H, BALB/c and C57BL/6J mice following acclimatization in metabolic cages

et al. 2010

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“…The slight increment (+7¢4%) in alkaline phosphatase activity observed after 72 h refrigeration in the absence of EDTA is a well documented phenomenon exhibited by lyophilized human-based QC serum following reconstitution, and has been reviewed elsewhere. 30 Alkaline phosphatases are zinc-containing metalloenzymes. 31 Preincubation of sample in the presence of EDTA leads to a pH-, temperature-and time-dependent irreversible inactivation of enzyme activity, which is thought to be due to the removal of functionally essential zinc atoms.…”

Section: Alkaline Phosphatase and Amylasementioning

confidence: 99%

“…30 Alkaline phosphatases are zinc-containing metalloenzymes. 31 Preincubation of sample in the presence of EDTA leads to a pH-, temperature-and time-dependent irreversible inactivation of enzyme activity, which is thought to be due to the removal of functionally essential zinc atoms.…”

Section: Alkaline Phosphatase and Amylasementioning

confidence: 99%

Effects of contamination of blood specimens with liquid potassium-EDTA anticoagulant

Davidson

2002

Ann Clin Biochem

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“…Optimized UV kinetic-test according to IFCC (International Federation of Clinical chemistry) was used to determine alanin amino transferase (ALT) (Huang et al, 2006). Alkaline phosphatase (ALP) was assayed according to Davidson (1979). The blood plasma malondialdehyde (MDA) level was determined by a method based on the reaction with thiobarbituric acid at 90-100C (Yagi, 1976).…”

Section: Methodsmentioning

confidence: 99%

The Effects of Pre-weaning and Post-weaning Feeding Periods on Biochemical Parameters in Terms of Metabolic Profile in Dorper and Lacaune Lambs

Budak¹

2023

IJAR

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Background: This study was conducted to evaluate the effects of feeding on biochemical parameters in terms of metabolic profile in female and male lambs of Dorper and Lacaune breeds at the developmental stages from birth. Methods: A total of 32 singleton newborn Dorper and Lacaune breed lambs (n= 8 for each group; male and female) were used. The trial was continued total of 5 nutritional periods each of 21-day after the first two weeks (0-14 days) suckling period. The biochemical parameters determined were paraoxonase 1 (PON1), ischemia modified albumin (IMA), total protein (TP), albumin (ALB), aspartat amino transferase (AST), alanin amino transferase (ALT), alkaline phosphatase (ALP) and malondialdehyde (MDA). Result: PON1 activity of all lambs was found to be low during the suckling period (P≤0.001). The highest IMA value was reached in against to the decrease in TP and ALP levels during the weaning period (P≤0.001). There was no difference between the groups in terms of PON1, TP, ALB and ALP. Increases were observed in the AST, ALT, IMA and MDA levels of male Dorpers. (P≤0.05). It was concluded that adaptation periods to solid feeds may cause oxidative damage or variable metabolic activity in male Dorpers.

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Measurement of serum alkaline phosphatase

Cited by 6 publications

References 74 publications

Establishing normal plasma and 24-hour urinary biochemistry ranges in C3H, BALB/c and C57BL/6J mice following acclimatization in metabolic cages

Establishing normal plasma and 24-hour urinary biochemistry ranges in C3H, BALB/c and C57BL/6J mice following acclimatization in metabolic cages

Effects of contamination of blood specimens with liquid potassium-EDTA anticoagulant

The Effects of Pre-weaning and Post-weaning Feeding Periods on Biochemical Parameters in Terms of Metabolic Profile in Dorper and Lacaune Lambs

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