Measurement of serum pralidoxime methylsulfate (Contrathion®) by high-performance liquid chromatography with electrochemical detection

Houzé, Pascal; Borron, Stephen W.; Scherninski, François; Bousquet, Bernard; Gourmel, Bernard; Baud, Frédéric J.

doi:10.1016/j.jchromb.2004.10.017

Cited by 30 publications

(28 citation statements)

References 20 publications

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“…The recovery could be improved by heat and/or chemical denaturation of the proteins in the blood serum. These procedures could be crucial for a correct determination of thiol concentrations in the sample [57,58].…”

Section: Determination Of the Thiols In Different Biological Matricesmentioning

confidence: 99%

Simultaneous determination of eight biologically active thiol compounds using gradient elution‐liquid chromatography with Coul‐Array detection

Petrlová¹,

Mikelová²,

Stejskal³

et al. 2006

J of Separation Science

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The most active form of sulfur in biomolecules is the thiol group, present in a number of biologically active compounds. Here we present a comprehensive study of thiol analysis using flow injection analysis/HPLC with electrochemical detection. The effect of different potentials of working electrodes, of organic solvent contents in the mobile phase, and of isocratic and gradient elution on simultaneous determination of thiol compounds (cysteine, cystine, N-acetylcysteine, homocysteine, reduced and oxidised glutathione, desglycinephytochelatin, and phytochelatins) are described and discussed. These thiol compounds were well separated and detected under optimised HPLC-electrochemical detection conditions (mobile phase: 80 mM trifluoroacetic acid and methanol with a gradient profile starting at 97:3 (TFA:methanol), kept constant for the first 8 min, then decreasing to 85:15 during one minute, kept constant for 8 min, and finally increasing linearly up to 97:3 from 17 to 18 min; the flow rate was 0.8 mL/min, column and detector temperature 25 degrees C, and the electrode potential 900 mV). We were able to determine tens of femtomoles (3 S/N) of the thiols per injection (5 microL), except for phytochelatin5 whose detection limit was 2.1 pmole. This technique was consequently used for simultaneous determination of compounds of interest in biological samples (maize tissue and human blood serum).

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Section: Determination Of the Thiols In Different Biological Matricesmentioning

confidence: 99%

Simultaneous determination of eight biologically active thiol compounds using gradient elution‐liquid chromatography with Coul‐Array detection

Petrlová¹,

Mikelová²,

Stejskal³

et al. 2006

J of Separation Science

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show abstract

“…The mass limits of detection were about 10 and 25 pg for the RAs and arROH, respectively. ECD has also been used for retinoid detection by others (Delgado Zamarreno et al, 1992;Iwahashi et al, 1987;MacCrehan and Schonberger, 1987a,b;Wring and Hart, 1989;Andreoli et al, 1997;Houze et al, 2004).…”

Section: Electrochemical Detection (Ecd)mentioning

confidence: 99%

Methods for detecting and identifying retinoids in tissue

Gundersen

2006

J. Neurobiol.

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Methods for retinoid analysis in tissue include direct spectrophotometry or fluorometry and retinoid responsive reporter constructs in the form of cell reporter assays or transgenic reporter animals, but chromatographic methods dominate and posses several superior features in quantitative analysis. The multitude of extraction protocols used can coarsely be divided into manual liquid-liquid extraction protocols and semi-or fully automated solid phase extraction-based protocols.Liquid chromatographic separation in reversed phase dominates although normal phase is also used. Detection is mainly performed with UV detectors although electrochemical and fluorescence detection is also used. Mass spectrometry in combination with LC is more often used in retinoid analysis and is likely to dominate in the future.

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“…[15][16][17][18][19] Cooper(II)-silica ligand exchange chromatography was used for determination of pralidoxime concentration in plasma and urine. 18 Later on, reversed-phase HPLC on octadecyl silica stationary phase was used for the determination of pralidoxime.…”

Section: Resultsmentioning

confidence: 99%

Time-dependent Changes in Concentration of Two Clinically used Acetylcholinesterase Reactivators (HI-6 and Obidoxime) in Rat Plasma Determined by HPLC Techniques after in vivo Administration

Karasová

Novotný²,

Antoš³

et al. 2010

ANAL. SCI.

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Measurement of serum pralidoxime methylsulfate (Contrathion®) by high-performance liquid chromatography with electrochemical detection

Cited by 30 publications

References 20 publications

Simultaneous determination of eight biologically active thiol compounds using gradient elution‐liquid chromatography with Coul‐Array detection

Simultaneous determination of eight biologically active thiol compounds using gradient elution‐liquid chromatography with Coul‐Array detection

Methods for detecting and identifying retinoids in tissue

Time-dependent Changes in Concentration of Two Clinically used Acetylcholinesterase Reactivators (HI-6 and Obidoxime) in Rat Plasma Determined by HPLC Techniques after in vivo Administration

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