Automatic replacement of bobbins on the frame is one of the core problems that must be solved in the textile industry. In the process of changing the bobbin, it is faced with problems such as the position offset of the bobbin, the loosening and deformation of the bobbin, which will lead to the failure of replacing the bobbin. Therefore, it is necessary to initialize the creel coordinates regularly, also considering the labor intensity and poor reliability of manual positioning. This paper proposes an automatic creel positioning method based on monocular vision. Firstly, the industrial camera was installed on the drum changing manipulator controlled by the truss system, and each yarn frame in the yarn area was inspected. Secondly, the end face image of the creel was collected at a reasonable distance, and the collected images were transmitted to the computer in real time through the Ethernet bus. Thirdly, the center coordinates (x, y) and radius r of the creel were marked by the improved Hough circle detection algorithm. Finally, the coordinate deviation of the creel was calculated and transmitted to the system controller to realize deviation correction. Before the test, the creel positioning markers were specially designed and the camera was calibrated. Thus, the influence of image complex background, creel end roughness, reflection and other factors can be reduced, and the image processing speed and positioning accuracy can be improved. The results show that the positioning effect of this method is fine when the distance between the center of the camera lens and the center of the end face of the creel is 170~190 mm. Moreover, when the distance is 190 mm, the positioning effect is the best, with an average error of only 0.51 mm. In addition, the deviation between the center coordinate and the radius of the end face of the marker is also very small, which is better than the requirements of bobbin yarn changing accuracy.