Measurement of soil microbial biomass phosphorus by an anion exchange membrane method

Effects of air-drying and freezing on phosphorus fractions in soils with different organic matter contents

“…The NaHCO 3 , NaOH and concentrated HCl extracts were divided into two aliquots in order to measure the total P and inorganic P. Organic P was calculated as a difference between total and inorganic P (Table 2). Biomass P (Pm) was determined by the fumigation extraction method after Kouno et al (1995). One-way ANOVA was carried out using the SPSS computer package (SPSS Inc. 1999, Chicago, USA) for all sets of data, and significant differences between means were determined through LSD test.…”

Priming and microbial nutrient limitation in lowland tropical forest soils of contrasting fertility

“…Total C and N in the extracts were determined by combustion and gas chromatography using a Thermo-Electron Flash 1112 Elemental Analyzer (CE Elantech, Lakewood, NJ). Readily-exchangeable phosphate (extractable P) and microbial P were determined by extraction with anion-exchange membranes and hexanol fumigation based on the method described by Kouno et al (1995). Phosphate was recovered from anion-exchange membranes by shaking for 1 h in 50 ml of 0.25 M H 2 SO 4 , with detection in the acid solution by automated molybdate colorimetry using a Lachat Quickchem 8500 (Hach Ltd, Loveland, CO, USA).…”

Stability of hydrolytic enzyme activity and microbial phosphorus during storage of tropical rain forest soils

“…Microbial phosphorus was determined by hexanol fumigation and extraction with anion-exchange membranes based on a method described by Kouno et al (1995) and Myers et al (1999). Anionexchange membrane strips (1 Â 4 cm; manufactured by BDH, Poole, UK; distributed by VWR International, West Chester, PA) were prepared in advance by initially shaking in 0.5 M NaHCO 3 .…”