Measurement of Soluble Adhesion Molecules: Can It Improve Diabetes Prediction?: Commentary on the article by Toivonen et al. on page 24

Wherrett, Diane K.

doi:10.1203/00006450-200101000-00005

Cited by 1 publication

(1 citation statement)

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“…ADAMTS-13 activity level was measured with an SDS–PAGE assay as previously described, using guanidine HCl treated vWF multimers as the substrate [13]. Proteolytic fragments generated from the substrate after incubation with a plasma sample were analyzed by SDS–PAGE and immunoblotting.…”

Section: Methodsmentioning

confidence: 99%

The influence of riboflavin photochemistry on plasma coagulation factors

Larrea¹,

Calabuig²,

Roldán

et al. 2009

Transfusion and Apheresis Science

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Studies with riboflavin in the 1960s showed that it could be effective at inactivating pathogens when exposed to light. The principal mode of action is through electron transfer reactions, most importantly in nucleic acids. This suggested that it could act as a photosensitizer useful in the inactivation of pathogens found in blood products.Objective-To study the influence of photo-inactivation with riboflavin on the coagulation factors of plasma.Methods-The photo-inactivation procedure of riboflavin plus light was applied. Fifty isogroup pools of two plasmas were made from 100 U of plasma that were derived from whole blood products that had previously been held overnight. Pools were split into two bags. One of them was photo-inactivated, and post inactivation samples were obtained. The second bag was not photoinactivated and samples were taken. Total protein, fibrinogen, FII, FV, FVII, FVIII, FIX, FX, FXI, FXIII, antithrombin III, PC, PS, α-2 antiplasmin and vWF:Ag, the multimeric structure of vWF and ADAMTS-13 were analyzed.Results-In plasma, the proteins most sensitive to photo-inactivation were fibrinogen, FXI, FVIII, FV, and FIX (33%, 32%, 30%, 18% and 18% loss, respectively). Coagulation inhibitors, PS, antithrombin III and PC showed little decrease (all 2%). Retention of vWF and ADAMTS-13 were 99% and 88%, respectively.Conclusions-As with other pathogen reduction procedures for plasma products, treatment with riboflavin and UV light resulted in reduction in the activity levels of several pro-coagulant factors. Coagulation inhibitors are well preserved.

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Section: Methodsmentioning

confidence: 99%