Measurement of ß2-Microglobulin in Serum by a Particle-Enhanced Nephelometric Immunoassay

Abstract

Summary:A particle-enhanced immunoassay of ß 2 -microglobulin in serum is described. It is based on the agglutination of complexes formed between the serum ß 2 -microglobulin and latex particles coated with F(ab') 2 fragments of polyclonal anti-ß 2 -microglobulin antibodies. The analytical ränge of the method is 0.50 to 16 mg/1; it can be extended by appropriate dihition to 0.12 to 80 mg/1 with good precision (CV less than 5% over the whole ränge). The accuracy and the precision are confirmed by a good correlation with radioimmunoassay (n = 123, r = 0.993). No error due to antigen excess was observed, even up to 292 mg/1.The main advantages of the method are its simplicity, its low cost per test and its high sensitivity (final dilution of the sample at 1/1200) with no known interference. The calibration curve is stable for at least 2 weeks.

A randomised, double blind, placebo controlled trial of the effect of theophylline in prevention of vasomotor nephropathy in very preterm neonates with respiratory distress syndrome

“…16 Serum theophylline concentrations were determined by the fluorescent polarisation immunoassay, 17 and b2 microglobulin by the nephelometric immunoassay. 18 Creatinine clearance was estimated using Schwartz's formula for preterm neonates [19][20][21] :…”

Détermination de la bêta-2-microglobuline dans les liquides biologiques par immunoanalyse : comparaison RIA, immunochémiluminescence et immunoturbidimétrie

“…Notre étude confirme que les méthodes d'immunoturbidimétrie et d'immunochémiluminescence sont des alternatives à la méthode de RIA [17,19,21,30]. En effet, les performan- ces analytiques de linéarité, de reproductibilité et de répéta-bilité sont satisfaisantes (Tableaux 1,2).…”