Measurement of the evolution of rigid and viscoelastic mass contributions from fibrin network formation during plasma coagulation using quartz crystal microbalance

Lakshmanan, Ramji S.; Efremov, Vitaly; Cullen, Sinéad M.; Killard, Anthony J.

doi:10.1016/j.snb.2013.10.081

Cited by 25 publications

(23 citation statements)

References 31 publications

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“…Moreover, in previous work, it has been shown that the respective final maximum values, Δ f max and Δ Г max , observed when clot formation was complete correlated well with fibrinogen concentration in PPP samples [11, 12]. However, the physical meaning and corresponding direct interpretation of MA( t ), Δ f ( t ) and Δ Г ( t ) are challenging.…”

Section: Resultsmentioning

confidence: 99%

Measurement of the viscoelastic properties of blood plasma clot formation in response to tissue factor concentration-dependent activation

et al. 2016

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The coagulation of blood plasma in response to activation with a range of tissue factor (TF) concentrations was studied with a quartz crystal microbalance (QCM), where frequency and half width at half maximum (bandwidth) values measured from the conductance spectrum near resonant frequency were used. Continuous measurement of bandwidth along with the frequency allows for an understanding of the dissipative nature of the forming viscoelastic clot, thus providing information on the complex kinetics of the viscoelastic changes occurring during the clot formation process. Using a mathematical model, these changes in frequency and bandwidth have been used to derive novel QCM parameters of effective elasticity, effective mass density and rigidity factor of the viscoelastic layer. The responses of QCM were compared with those from thromboelastography (TEG) under identical conditions. It was demonstrated that the nature of the clot formed, as determined from the QCM parameters, was highly dependent on the rate of clot formation resulting from the TF concentration used for activation. These parameters could also be related to physical clot characteristics such as fibrin fibre diameter and fibre density, as determined by scanning electron microscopic image analysis. The maximum amplitude (MA) as measured by TEG, which purports to relate to clot strength, was unable to detect these differences.

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Section: Resultsmentioning

confidence: 99%

Measurement of the viscoelastic properties of blood plasma clot formation in response to tissue factor concentration-dependent activation

et al. 2016

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“…Furthermore, the blood proteins, especially fibrinogen and fibrin fibers, behave not viscously but rigidly after their attachment for a wide range of coating materials (Andersson et al 2005). When the blood start to clot, not only the change in viscosity but also the change in elasticity and the fibrin adhesion may occur simultaneously as the result of the formation of fibrin fibers (Lakshmanan et al 2014). In addition, other factors, such as the nonhomogeneous shear vibration, boundary effect and micro-form standing wave for the ultra-small sample volume, may influence the resonant state of the FBAR sensors.…”

Section: Discussionmentioning

confidence: 99%

Micro-electromechanical film bulk acoustic sensor for plasma and whole blood coagulation monitoring

Chen

Song

et al. 2017

Biosensors and Bioelectronics

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Monitoring blood coagulation is an important issue in the surgeries and the treatment of cardiovascular diseases. In this work, we reported a novel strategy for the blood coagulation monitoring based on a micro-electromechanical film bulk acoustic resonator. The resonator was excited by a lateral electric field and operated under the shear mode with a frequency of 1.9GHz. According to the apparent step-ladder curves of the frequency response to the change of blood viscoelasticity, the coagulation time (prothrombin time) and the coagulation kinetics were measured with the sample consumption of only 1μl. The procoagulant activity of thromboplastin and the anticoagulant effect of heparin on the blood coagulation process were illustrated exemplarily. The measured prothrombin times showed a good linear correlation with R=0.99969 and a consistency with the coefficient of variation less than 5% compared with the commercial coagulometer. The proposed film bulk acoustic sensor, which has the advantages of small size, light weight, low cost, simple operation and little sample consumption, is a promising device for miniaturized, online and automated analytical system for routine diagnostics of hemostatic status and personal health monitoring.

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“…Various optical or mechanical methods, such as surface plasmon resonance [31], quartz crystal microbalance [32], surface acoustic waves [33] or ultrasounds [34], capillary migration [35], magnetic bead movement detection [36] or conductivity measurement, have been reported in the literature to measure blood coagulation [37][38][39]. Some of them have been developed thoroughly, especially the conductivity measurement, and led to devices currently on the market.…”

Section: Discussionmentioning

confidence: 99%

Whole blood spontaneous capillary flow in narrow V-groove microchannels

Berthier

Brakke

Furlani

et al. 2015

Sensors and Actuators B: Chemical

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Measurement of the evolution of rigid and viscoelastic mass contributions from fibrin network formation during plasma coagulation using quartz crystal microbalance

Cited by 25 publications

References 31 publications

Measurement of the viscoelastic properties of blood plasma clot formation in response to tissue factor concentration-dependent activation

Measurement of the viscoelastic properties of blood plasma clot formation in response to tissue factor concentration-dependent activation

Micro-electromechanical film bulk acoustic sensor for plasma and whole blood coagulation monitoring

Whole blood spontaneous capillary flow in narrow V-groove microchannels

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