Measurement of the influence of flavonoids on DNA repair kinetics using the comet assay

Charles, Catherine; Chemais, Monia; Stévigny, Caroline; Dubois, Jacques; Nachergael, Amandine; Duez, Pierre

doi:10.1016/j.foodchem.2012.06.112

Cited by 17 publications

(15 citation statements)

References 43 publications

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“…Charles et al (2012) used the comet assay to measure the influence on DNA repair kinetics of different flavonoids at the highest non-toxic concentration. The flavonoids have the capacity to modulate DNA repair and this ability was tested (Charles et al 2012). Similar studies were also carried out by other teams (Azqueta et al 2014, Silva et al 2009.…”

Section: Modification Of the Comet Assaymentioning

confidence: 75%

Comet assay and its use for evaluating oxidative DNA damage in some pathological states

et al. 2019

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The comet assay, or single-cell gel electrophoresis (SCGE), is a sensitive, rapid, relatively simple and inexpensive method for detecting DNA strand breaks in individual cells. It is used in a broad variety of applications and as a tool to investigate DNA damage and repair. The sensitivity and specificity of the assay are greatly enhanced if the DNA incubated with an enzyme, whichrecognizes a specific kind of DNA damage. This damage induced by oxidative stress plays a pivotal role in many diseases and in aging. This article is a critical review of the possible application of the comet assay in some pathological states in clinical practice. Most of the studies relate to evaluating the response of an organism to chemotherapy or radiotherapy with statistically significant evidence of DNA damage in patients. Other useful applications have been demonstrated for patients with heart or neurodegenerative diseases. Only a few studies have been published on the use of this method in critically ill patients, although its use would be appropriate. There are also other scenarios where the comet assay could prove to be very useful in the future, such as in predicting the likelihood of certain pathological conditions.

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Section: Modification Of the Comet Assaymentioning

confidence: 75%

Comet assay and its use for evaluating oxidative DNA damage in some pathological states

et al. 2019

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“…Some aromatic plants also contain polyphenols, compounds that have shown special interest over the last few years, due to the great variety of biological properties most of them display, notably for anti-carcinogenic, anti-inflammatory, antiviral, anti-allergic, estrogenic, and immune-stimulating effects (Atoui et al, 2005). From these, flavonoids have been extensively investigated for their possible roles in cancer chemoprevention (Charles et al, 2012). Many of these activities have been associated with the metal chelation and redox properties of polyphenols, which allow them to act as antioxidants, hydrogen donators, and reactive oxygen species quenchers (Kaisoon et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Phenolic composition, in vitro antioxidant effects and tyrosinase inhibitory activity of three Algerian Mentha species: M. spicata (L.), M. pulegium (L.) and M. rotundifolia (L.) Huds (Lamiaceae)

Brahmi

Hauchard

Guendouze

et al. 2015

Industrial Crops and Products

131

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International audienceThe leaves of three Mentha species harvested in Algeria, Mentha spicata L. (MS), Mentha pulegium L. (MP) and Mentha rotundifolia (L.) Huds (MR) were examined for their content in polyphenols and for some activities-linked biological properties these could impart. The contents in total phenolics (TPC) and flavonoids (TFC) were evaluated by the Folin–Ciocalteu and the aluminum chloride methods, respectively. Whereas MS showed the highest TPC (12.0 ± 0.3 mg gallic acid equivalents/g of dry weight), MR had the highest content in TFC (3.3 ± 0.1 mg quercetin equivalents of dry weight). The pharmacological properties of these extracts were evaluated by assessing in vitro their antioxidant and antityrosinase activities. The modulation of mushroom tyrosinase activity was measured by colorimetry of the melanins formed in the presence of tyrosine. MS exhibited the strongest radical scavenging activity (RSA) in all assays: (i) the IC50s values to neutralize the 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) cation radicals (ABTS+) and the 2,2′-diphenyl-1-picrylhydrazyl radicals (DPPH) were 10.3 ± 0.9 and 16.2 ± 0.2 μg/mL, respectively; and (ii) its original electrochemically measured superoxide quenching index value is 188 ± 37 μg/mL (AI50). MR however showed the highest tyrosinase inhibitory activity (IC50 = 108 ± 20 μg/mL). A silica gel thin-layer chromatography (TLC) technique revealed the presence of caffeic and rosmarinic acids and diosmin in all extracts. These results were confirmed by high performance liquid chromatography with diode array detection (HPLC/DAD

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“…At 24 h, the flavone apigenin was found to reduce DNA fragmentation. The flavonol quercetin substantially increases DNA fragmentation at 12 h which may correspond either to direct DNA damage or to the induction of non-specific endonuclease activity [58].…”

Section: Recents Studies Using Comest Assaymentioning

confidence: 99%

Comet Assay on Toxicogenetics; Several Studies in Recent Years on Several Genotoxicological Agents

Gharsalli¹

2016

J Environ Anal Toxicol

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Measurement of the influence of flavonoids on DNA repair kinetics using the comet assay

Cited by 17 publications

References 43 publications

Comet assay and its use for evaluating oxidative DNA damage in some pathological states

Comet assay and its use for evaluating oxidative DNA damage in some pathological states

Phenolic composition, in vitro antioxidant effects and tyrosinase inhibitory activity of three Algerian Mentha species: M. spicata (L.), M. pulegium (L.) and M. rotundifolia (L.) Huds (Lamiaceae)

Comet Assay on Toxicogenetics; Several Studies in Recent Years on Several Genotoxicological Agents

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