Measurement of the QT Interval: Lessons from Thirty-Two Animal Species for the Correction of the QT Interval by Heart Rate

W, Rabkin Simon

doi:10.23937/2378-2951/1410127

Int J Clin Cardiol

2018

DOI: 10.23937/2378-2951/1410127

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Measurement of the QT Interval: Lessons from Thirty-Two Animal Species for the Correction of the QT Interval by Heart Rate

Rabkin Simon W¹

Abstract: Background: The optimal method to correct the QT interval, an important indication of arrhythmia risk, for the impact of heart rate has been controversial. The fundamental biologic relationship of the QT interval-heart rate relationship has rarely been studied across different species. Methods: The literature on the QT and heart rate of 32 different species was examined specifically in the whales, dol

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2022

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Comparison of Sources and Methods for the Isolation of Equine Adipose Tissue-Derived Stromal/Stem Cells and Preliminary Results on Their Reaction to Incubation with 5-Azacytidine

Trachsel

Stage

Rausch

et al. 2022

Animals

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Physiological particularities of the equine heart justify the development of an in vitro model suitable for investigations of the species-specific equine cardiac electrophysiology. Adipose tissue-derived stromal/stem cells (ASCs) could be a promising starting point from which to develop such a cardiomyocyte (CM)-like cell model. Therefore, we compared abdominal, retrobulbar, and subcutaneous adipose tissue as sources for the isolation of ASCs applying two isolation methods: the collagenase digestion and direct explant culture. Abdominal adipose tissue was most suitable for the isolation of ASCs and both isolation methods resulted in comparable yields of CD45-/CD34-negative cells expressing the mesenchymal stem cell markers CD29, CD44, and CD90, as well as pluripotency markers, as determined by flow cytometry and real-time quantitative PCR. However, exposure of equine ASCs to 5-azacytidine (5-AZA), reportedly inducing CM differentiation from rats, rabbits, and human ASCs, was not successful in our study. More precisely, neither the early differentiation markers GATA4 and NKX2-5, nor the late CM differentiation markers TNNI3, MYH6, and MYH7 were upregulated in equine ASCs exposed to 10 µM 5-AZA for 48 h. Hence, further work focusing on the optimal conditions for CM differentiation of equine stem cells derived from adipose tissue, as well as possibly from other origins, are needed.

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Comparison of Sources and Methods for the Isolation of Equine Adipose Tissue-Derived Stromal/Stem Cells and Preliminary Results on Their Reaction to Incubation with 5-Azacytidine

Trachsel

Stage

Rausch

et al. 2022

Animals

View full text Add to dashboard Cite

show abstract

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Measurement of the QT Interval: Lessons from Thirty-Two Animal Species for the Correction of the QT Interval by Heart Rate

Cited by 1 publication

References 63 publications

Comparison of Sources and Methods for the Isolation of Equine Adipose Tissue-Derived Stromal/Stem Cells and Preliminary Results on Their Reaction to Incubation with 5-Azacytidine

Comparison of Sources and Methods for the Isolation of Equine Adipose Tissue-Derived Stromal/Stem Cells and Preliminary Results on Their Reaction to Incubation with 5-Azacytidine

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