Measurement of Total and Bioactive Interleukin-2 in Tissue Samples by Immunoaffinity-Receptor Affinity Chromatography

Phillips, Terry M.

doi:10.1002/(sici)1099-0801(199707)11:4<200::aid-bmc674>3.0.co;2-h

Cited by 13 publications

(5 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The following probes were used for whole-mount in situ hybridization: 5’-GCCTGGCAGCGAATGCGGTCCAGAAA GAAAGACAA-3’; 5’-AGGGGCCAGGGCGTGCACTACTGGATCAGAACATT-3’, 5’-GACTACGACCAGTTTGCCATGGTATTTTTCCAGGA-3’. Dewaxing, rehydration, and antigen retrieval were performed as previously described [20]. The sections were then incubated in diethyl pyrocarbonate (0.1 % DEPC)-treated PBS and fixed in 4 % paraformaldehyde in PBS for 10 minutes.…”

Section: Methodsmentioning

confidence: 99%

Renal neutrophil gelatinase associated lipocalin expression in lipopolysaccharide-induced acute kidney injury in the rat

Han

Liu

et al. 2012

BMC Nephrol

View full text Add to dashboard Cite

BackgroundNeutrophil gelatinase associated lipocalin (NGAL) is a highly predictive biomarker of acute kidney injury. To understand the role of NGAL in renal injury during sepsis, we investigated the temporal changes and biological sources of NGAL in a rat model of acute kidney injury, and explored the relationship between renal inflammation, humoral NGAL and NGAL expression during endotoxemia.MethodsTo induce acute renal injury, rats were treated with lipopolysaccharide (LPS, 3.5 mg/kg, ip), and the location of NGAL mRNA was evaluated by in situ hybridization. Quantitative RT-PCR was also used to determine the dynamic changes in NGAL, tumor necrosis factor α (TNFα) and interleukin (IL)-6 mRNA expression 1, 3, 6, 12, and 24 hours following LPS treatment. The correlation among NGAL, TNFα and IL-6 was analyzed. Urinary and plasma NGAL (u/pNGAL) levels were measured, and the relationship between humoral NGAL and NGAL expression in the kidney was investigated.ResultsRenal function was affected 3–12 hours after LPS. NGAL mRNA was significantly upregulated in tubular epithelia at the same time (P < 0.001). The course of NGAL mRNA upregulation occurred in parallel with renal damage. There was a transient increase in TNFα and IL-6 mRNA levels within 3 hours following LPS administration, and a strong correlation between TNFα and NGAL mRNA (r = 0.995, P <0.001) but not with IL-6 mRNA. Both pNGAL and uNGAL levels were markedly increased compared with those in the control group (P < 0.001); however, only uNGAL levels were correlated with NGAL mRNA (r = 0.850, P <0.001).ConclusionsNGAL upregulation is sensitive to LPS-induced renal TNFα increase and injury, which are observed in the tubular epithelia. Urinary NGAL levels accurately reflect changes in NGAL in the kidney.

show abstract

Section: Methodsmentioning

confidence: 99%

Renal neutrophil gelatinase associated lipocalin expression in lipopolysaccharide-induced acute kidney injury in the rat

Han

Liu

et al. 2012

BMC Nephrol

View full text Add to dashboard Cite

show abstract

“…Since then, numerous applications have been published using immobilized antibodies as affinity ligands [24-29, 41-46, 49, 54, 55, 130-138]. Phillips and coworkers [139-150] have also been important pioneers in the field of IALC and IACE. Numerous applications employing IALC or IACE include potentially useful clinical-diagnosis-prognosis tests [24, 26-29, 39-46, 151].…”

Section: Other Immunoaffinity Capillary Electrophoresis Applicationsmentioning

confidence: 99%

Immunoaffinity capillary electrophoresis as a powerful strategy for the quantification of low‐abundance biomarkers, drugs, and metabolites in biological matrices

2008

Self Cite

View full text Add to dashboard Cite

In the last few years, there has been a greater appreciation by the scientific community of how separation science has contributed to the advancement of biomedical research. Despite past contributions in facilitating several biomedical breakthroughs, separation sciences still urgently need the development of improved methods for the separation and detection of biological and chemical substances. In particular, the challenging task of quantifying small molecules and biomolecules, found in low abundance in complex matrices (e.g., serum), is a particular area in need of new highefficiency techniques. The tandem or on-line coupling of highly selective antibody capture agents with the high-resolving power of CE is being recognized as a powerful analytical tool for the enrichment and quantification of ultra-low abundance analytes in complex matrices. This development will have a significant impact on the identification and characterization of many putative biomarkers and on biomedical research in general. Immunoaffinity CE (IACE) technology is rapidly emerging as the most promising method for the analysis of low-abundance biomarkers; its power comes from a three-step procedure: (i) bioselective adsorption and (ii) subsequent recovery of compounds from an immobilized affinity ligand followed by (iii) separation of the enriched compounds. This technology is highly suited to automation and can be engineered to as a multiplex instrument capable of routinely performing hundreds of assays per day. Furthermore, a significant enhancement in sensitivity can be achieved for the purified and enriched affinity targeted analytes. Thus, a compound that exists in a complex biological matrix at a concentration far below its LOD is easily brought to well within its range of quantification. The present review summarizes several applications of IACE, as well as a chronological description of the improvements made in the fabrication of the analyte concentrator-microreactor device leading to the development of a multidimensional biomarker analyzer.

show abstract

“…Creatinine was quantitated in unretained peaks to correct for fluc- tuations in the concentration of albumin due to variations in urine output and volume. Another combined detection method was reported in which an antibody column was followed by an immobilized receptor cartridge to measure the total and bioactive interleukin-2 in tissue samples as an aid in the diagnosis of organ transplant rejection (41).…”

Section: P Po Os St Tc Co Ol Lu Um Mn N I Im Mm Mu Un No Od De Et Te mentioning

confidence: 99%

Peer Reviewed: Chromatographic Immunoassays

Hage¹,

Nelson²

2001

Anal. Chem.

View full text Add to dashboard Cite

Measurement of Total and Bioactive Interleukin-2 in Tissue Samples by Immunoaffinity-Receptor Affinity Chromatography

Cited by 13 publications

References 21 publications

Renal neutrophil gelatinase associated lipocalin expression in lipopolysaccharide-induced acute kidney injury in the rat

Renal neutrophil gelatinase associated lipocalin expression in lipopolysaccharide-induced acute kidney injury in the rat

Immunoaffinity capillary electrophoresis as a powerful strategy for the quantification of low‐abundance biomarkers, drugs, and metabolites in biological matrices

Peer Reviewed: Chromatographic Immunoassays

Contact Info

Product

Resources

About