Measurement of total and differential white blood cell counts in synovial fluid by means of an automated hematology analyzer

Hyaluronidase treatment of synovial fluid to improve assay precision for biomarker research using multiplex immunoassay platforms

“…Studies on flow-cytometer cell counting in SF samples are of particular relevance to our results on the Luminex platform (Moreno et al, 2000;Sugiuchi et al, 2005;Ekmann et al, 2010). These studies demonstrate that HAse treatment improved the precision by reducing errors due to high viscosity related flow.…”

“…These properties can make the analysis of SF from OA joints challenging. Consequently, methods to reduce SF viscosity by dilution and/or hyaluronidase (HAse) treatment are routinely used before a variety of analytic techniques (Song et al, 2012;Mateos et al, 2012;Sugiuchi et al, 2005;de Jager et al, 2007;Ekmann et al, 2010).…”

Líquido sinovial da articulação femuropatelar após desmotomia patelar medial experimental em eqüinos

“…Como a viscosidade do líquido sinovial está diretamente relacionada ao conteúdo de hialuronato (SUGIUCHI et al, 2005;TULAMO et al, 1994;TULAMO et al, 1996), a diminuição observada no 30 o po é sugestiva de trauma articular causado pela desmotomia patelar medial. Segundo SUGIUCHI et al (2005), na presença de inflamação, as enzimas no líquido sinovial quebram a molécula de hialuronato, o volume de líquido sinovial aumenta e a viscosidade diminuiu.…”

A 3-Day Delay in Synovial Fluid Crystal Identification Did Not Hinder the Reliable Detection of Monosodium Urate and Calcium Pyrophosphate Crystals

“…In the following hour, the specimen was analyzed by a second blinded observer in the same way. Every day, a new slide was prepared; native SF was pretreated with hyaluronidase to disintegrate clots (H3506 Type I-S from Bovine Tests, Fa; Sigma-Aldrich, St Louis, MO) as described by Sugiuchi et al 11 Daily crystal analysis was conducted blinded, which means that each fluid was examined by a different analyst each successive day and without the knowledge of earlier results. The results were documented independently and fixed in a protocol by an uninvolved third person (medical technician).…”