Measurement of total HDL, HDL2 and HDL3 by dextran sulfate—MgCl2 precipitation technique in human serum

Talameh, Y.; Wei, Robert; Naito, Herbert K.

doi:10.1016/0009-8981(86)90113-0

Cited by 52 publications

(19 citation statements)

References 13 publications

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“…HDL-cholesterol was measured after precipitation of apo B-containing lipoproteins using dextran sulfate and magnesium chloride, and HDL2 and HDL3 subfractions separated by differential precipitation. 28 LDL-cholesterol was calculated according to Friedewald et al 29 Apo A-l, and apo B were measured by immunoturbidometric methods (Incstar, Stillwater, MN, USA) on the COBAS analyzer, and Lp(a) was assayed using an ELISA (Perlmmune). LDL particle size was measured by non-denaturing polyacrylamide gels electrophoresis 30 and HbA1c was assayed by a standard high pressure liquid chromatographic method (Biorad Labs, Hercules, CA, USA).…”

Section: Methodsmentioning

confidence: 99%

Impact of an ACE inhibitor and calcium antagonist on microalbuminuria and lipid subfractions in type 2 diabetes: a randomised, multi-centre pilot study

Bakris¹,

Smith²,

Richardson³

et al. 2002

J Hum Hypertens

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Background: Microalbuminuria (MA) is associated with increased cardiovascular risk and lipid abnormalities in people with type 2 diabetes. ACE inhibitors and calcium channel blockers (CCBs) reduce MA and are neutral on total cholesterol and triglycerides. The effect of ACE inhibitors and CCBs on lipid subfractions such as Lp(a), apolipoprotein (apo) A1, apo B, and others, however, is unclear. The current study tests the hypothesis that a fixed-dose combination of an ACE inhibitor, benazepril (B) with the dihydropyridine CCB, amlodipine (A), will further reduce arterial pressure and reduce atherogenic lipid fractions compared to either agent alone. Design: A multicentre, randomised, open-label, parallel group design was used to study 27 participants with type 2 diabetes. Measurements for total cholesterol, high-and low-density lipoprotein (HDL and LDL), triglycerides, apo A1, apo B, Lp(a), MA, arterial pressure and creatinine clearance were obtained at baseline and at 12-week intervals during the 36 week study. Results: Arterial pressure was significantly reduced at 36 weeks in all three groups (P ‫؍‬ 0.0078 for A, P ‫؍‬

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Section: Methodsmentioning

confidence: 99%

Impact of an ACE inhibitor and calcium antagonist on microalbuminuria and lipid subfractions in type 2 diabetes: a randomised, multi-centre pilot study

Bakris¹,

Smith²,

Richardson³

et al. 2002

J Hum Hypertens

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show abstract

“…HDL-cholesterol fractions were obtained by dextran sulfatemagnesium chloride precipitation and assayed using a commercial kit (Daiichi, Tokyo, Japan). 12 Separation of lipoproteins by ultracentrifugation was performed as described by Havel et al 13 Plasma remnant-like particle (RLP)-cholesterol was determined by immunoabsorption using the commercial RLP-C JIMRO kit. 14 Plasma cholesteryl ester transfer protein (CETP) concentrations were determined by enzyme-linked immunosorbent assay using the monoclonal antibody TP2 and a rabbit polyclonal antibody raised against recombinant human CETP.…”

Section: Laboratory Analysesmentioning

confidence: 99%

High Frequency of a Retinoid X Receptor γ Gene Variant in Familial Combined Hyperlipidemia That Associates With Atherogenic Dyslipidemia

Nohara

Kawashiri

Claudel

et al. 2007

ATVB

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“…High-density lipoprotein cholesterol was determined with the dextransulfate/Mg 2+ precipitation technique, 19 apolipoproteins by electroimmunoassay, 20 and serum parathyroid hormone by radioimmunoassay.…”

Section: Laboratory Evaluationmentioning

confidence: 99%

Wave reflections and cardiac hypertrophy in chronic uremia. Influence of body size.

Marchais¹,

Guérin²,

Pannier³

et al. 1993

Hypertension

225

119

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Arterial wave reflections (AWRs), an important determinant of cardiac afterload, are increased in hemodialysis patients. However, an association between AWRs and left ventricular hypertrophy has not been established in these subjects. We therefore sought an association between these two parameters in a cross-sectional study of two outpatient hemodialysis populations. AWRs were quantified (augmentation index, percent) as the ratio of the height of the late systolic peak to the total height of the carotid pulse wave recorded with a micromanometer-tipped probe. AWRs were determined in 44 hemodialysis patients, 22 with pronounced AWRs (group A: augmentation index >12%), and 22 with small or moderate AWRs (group B: augmentation index <12%). The groups were matched for age, sex, blood pressure, and hemodialysis duration. Left ventricular size was determined by echocardiography. Despite lower body size (P<.005) and similar blood pressure, cardiac output, peripheral resistance, and aortic pulse wave velocity, group A patients had greater left ventricular mass (P<.01). For the population as a whole, left ventricular mass and AWRs were positively correlated (P<.0001) independent of age, blood pressure, hemodialysis duration, or body size. Lesser body height was the principal factor associated with increased AWRs in group A (P<.001). We conclude that in hemodialysis patients AWRs are associated with the development of left ventricular hypertrophy and that small body height is a risk factor for long-term cardiovascular complications. 13 Both nonhemodynamic factors and hemodynamic alterations due to chronic flow and pressure overload play a role in LVH development in these patients. 13 Even though hypertension is held responsible for such a high prevalence, LVH develops and progresses with time on hemodialysis and is only loosely correlated with blood pressure (BP) level, at least as reflected by usual BP measurements. 13 However, systolic BP (SBP) and diastolic BP (DBP) are merely the limits of BP fluctuation during the cardiac cycle and not the best expression of the load against which the left ventricle must work. Indeed, SBP in the left ventricle or aorta is also determined by ventricular ejection and to a degree is dependent on preload. -5Aortic input impedance, determined by arteriolar tone, aortic distensibility and diameter, and the intensity and timing of arterial wave reflections (AWRs), provides valuable information concerning the characteristics of the arterial system in accepting pulsatile flow from the

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Measurement of total HDL, HDL2 and HDL3 by dextran sulfate—MgCl2 precipitation technique in human serum

Cited by 52 publications

References 13 publications

Impact of an ACE inhibitor and calcium antagonist on microalbuminuria and lipid subfractions in type 2 diabetes: a randomised, multi-centre pilot study

Impact of an ACE inhibitor and calcium antagonist on microalbuminuria and lipid subfractions in type 2 diabetes: a randomised, multi-centre pilot study

High Frequency of a Retinoid X Receptor γ Gene Variant in Familial Combined Hyperlipidemia That Associates With Atherogenic Dyslipidemia

Wave reflections and cardiac hypertrophy in chronic uremia. Influence of body size.

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