Measurement of total testosterone in women: comparison of a direct radioimmunoassay versus radioimmunoassay after organic solvent extraction and celite column partition chromatography

Davison, Sonia Louise; Bell, Robin Jean; Montalto, Joseph; Sikaris, Ken; Donath, Susan; Stanczyk, Frank Z.; Davis, Susan R.

doi:10.1016/j.fertnstert.2005.05.058

Cited by 29 publications

(18 citation statements)

References 14 publications

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“…In women, ovarian aging is characterized by a reduced ovarian sensitivity to FSH, which is accompanied by a progressive decline in serum androgen levels (Davison et al 2005, Sowers et al 2009). Several studies show that women with an elevated FSH concentration, independent of age, have a poor response to ovarian stimulation, leading to a lower pregnancy rate using assisted reproduction techniques (Scott et al 1989, Martin et al 1996, Sharif et al 1998, El-Toukhy et al 2002.…”

Section: Discussionmentioning

confidence: 99%

Evidence that high variation in antral follicle count during follicular waves is linked to alterations in ovarian androgen production in cattle

Mossa¹,

Jimenez-Krassel²,

Folger³

et al. 2010

Reproduction

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Androgens have an important role in ovarian follicular growth and function, but circulating androgen concentrations are also associated with ovarian dysfunction, cardiovascular disease, and metabolic disorders in women. The extent and causes of the variation in androgen production in individuals, however, are unknown. Because thecal cells of follicles synthesize androstenedione and testosterone, variation in production of these androgens is hypothesized to be directly related to the inherently high variation in number of healthy growing follicles in ovaries of individuals. To test this hypothesis, we determined whether thecal CYP17A1 mRNA (codes for a cytochrome P450 enzyme involved in androgen synthesis), LH-induced thecal androstenedione production, androstenedione concentrations in follicular fluid, and circulating testosterone concentrations were lower in cattle with relatively low versus high number of follicles growing during follicular waves and whether ovariectomy reduced serum testosterone concentrations. Results demonstrated that cattle with a low follicle number had lower (P!0.05) abundance of CYP17A1 mRNA in thecal cells, reduced (P!0.01) capacity of thecal cells to produce androstenedione in response to LH, lower (P!0.01) androstenedione concentrations in ovulatory follicles, and lower (P!0.02) circulating testosterone concentrations during estrous cycles compared with animals with high follicle number. Also, serum testosterone in cattle with low or high follicle number was reduced by 63 and 70%, respectively, following ovariectomy. In conclusion, circulating androgen concentrations are lower in cattle with low versus high number of follicles growing during follicular waves, possibly because of a reduced responsiveness of thecal cells to LH.

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Section: Discussionmentioning

confidence: 99%

Evidence that high variation in antral follicle count during follicular waves is linked to alterations in ovarian androgen production in cattle

Mossa¹,

Jimenez-Krassel²,

Folger³

et al. 2010

Reproduction

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“…According to many authors calculation of free androgen levels using total testosterone measurement by means of direct radioimmunoassay is regarded as a suitable alternative method of assessing testosterone availability [32]. More recently, radioimmunoassay after organic solvent extraction was introduced as a more accurate method to measure total testosterone [33]. Although total testosterone and free androgen levels using conventional measures are invalid to make inferences about the extent to which relevant tissues are androgenized on an individual level, such measurements do enable us to control for the possibility that differences in these hormones are an obvious explanation for differences in sexual function between groups [34].…”

Section: Discussionmentioning

confidence: 99%

Genital Responsiveness in Healthy Women With and Without Sexual Arousal Disorder

Laan

Driel

Lunsen

2008

The Journal of Sexual Medicine

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Introduction Most pharmacological treatments that are currently being developed for women with sexual arousal disorder are aimed at remedying a vasculogenic deficit. Aim This study investigated whether pre- and postmenopausal women with sexual arousal disorder are less genitally responsive to visual sexual stimuli than pre- and postmenopausal women without sexual problems. Method Twenty-nine medically healthy women with sexual arousal disorder (15 premenopausal and 14 postmenopausal), diagnosed using the Diagnostic and Statistical Manual of Mental Disorders, 4th Edition (DSM-IV) criteria, and 30 age-matched women without sexual problems (16 premenopausal and 14 postmenopausal) were shown sexual stimuli depicting cunnilingus and intercourse. Main Outcome Measure Genital arousal was assessed as vaginal pulse amplitude (VPA) using vaginal photoplethysmography. Results Results showed no significant differences between the two groups in mean and maximum VPA, nor in latency of VPA response. Conclusion Women with sexual arousal disorder diagnosed according to DSM-IV criteria were not less genitally responsive to visual sexual stimuli than women without such problems. These findings are in line with previous studies. The sexual problems these women report are clearly not related to their potential to become genitally aroused. We argue that the DSM-IV criteria for sexual arousal disorder are in need of revision. In medically healthy women, impaired genital responsiveness is not a valid diagnostic criterion.

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“…However, a testosterone level should be measured prior to therapy primarily to exclude women who may be at risk of side‐effects if treated (see below). Total testosterone is notoriously difficult to measure at lower levels, as seen in women with sensitivity and precision 33 . Total testosterone and SHBG should be measured and from these, free testosterone can be reliably estimated using the Sodergard equation 34 .…”

Section: Appropriate Candidates For Testosterone Therapymentioning

confidence: 99%

“…Total testosterone is notoriously difficult to measure at lower levels, as seen in women with sensitivity and precision. 33 Total testosterone and SHBG should be measured and from these, free testosterone can be reliably estimated using the Sodergard equation. 34 The measurement of free testosterone in women by direct 'kit' assays is generally completely unreliable.…”

Section: Appropriate Candidates For Testosterone Therapymentioning

confidence: 99%

Should women receive androgen replacement therapy, and if so, how?

Davis

2010

Clinical Endocrinology

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The available clinical evidence supports efficacy of testosterone therapy for the treatment of postmenopausal women with hypoactive sexual desire disorder (HSDD) who have undergone a comprehensive clinical evaluation. Although few preparations designed to deliver an appropriate dose of testosterone for women are available, use of testosterone by women for the management of HSDD is widespread. Issues that continue to simulate debate in this therapeutic area include whether HSDD is a condition that merits pharmacotherapy, how effective is such treatment and whether testosterone therapy is safe. Hence the question, should women receive androgen replacement therapy, and if so, how?

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Measurement of total testosterone in women: comparison of a direct radioimmunoassay versus radioimmunoassay after organic solvent extraction and celite column partition chromatography

Cited by 29 publications

References 14 publications

Evidence that high variation in antral follicle count during follicular waves is linked to alterations in ovarian androgen production in cattle

Evidence that high variation in antral follicle count during follicular waves is linked to alterations in ovarian androgen production in cattle

Genital Responsiveness in Healthy Women With and Without Sexual Arousal Disorder

Should women receive androgen replacement therapy, and if so, how?

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