Measurement of trazodone in plasma and brain of rat by capillary gas chromatography with a nitrogen-selective detector

Andriollo, O.; Lartigue-Mattei, C.; Chabard, J. L.; Bargnoux, H.; Petit, J.; Berger, J.A.; Pognat, J F

doi:10.1016/0378-4347(92)80161-i

Cited by 11 publications

(3 citation statements)

References 10 publications

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“…Several chromatographic methods have been presented for the estimation of trazodone in biological fluids employing HPLC-UV [8,9], HPLC-fluorescence [10,11], gas chromatography with nitrogen selective [12] and mass spectrometric detection [13,14]. Some of these methods suffer from various drawbacks namely sensitivity, long chromatographic run times, large sample volume for processing or a cumbersome extraction procedure, which prevent their use for routine subject sample analysis.…”

Section: Introductionmentioning

confidence: 99%

High throughput and sensitive determination of trazodone and its primary metabolite, m-chlorophenylpiperazine, in human plasma by liquid chromatography–tandem mass spectrometry

Patel

Sharma²,

Sanyal

et al. 2008

Journal of Chromatography B

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Section: Introductionmentioning

confidence: 99%

High throughput and sensitive determination of trazodone and its primary metabolite, m-chlorophenylpiperazine, in human plasma by liquid chromatography–tandem mass spectrometry

Patel

Sharma²,

Sanyal

et al. 2008

Journal of Chromatography B

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“…At present, various methods have been reported for determining TZD concentrations in human plasma, including methods using HPLC, 5,[12][13][14][15] capillary gas chromatography, 16 gas chromatography-MS/MS, [17][18][19] LC, 20 and LC-MS method. [21][22][23][24][25][26][27] However, these methods are limited by their low detection sensitivity, long chromatographic run times, the requirement of large sample volumes for processing, and complicated sample pretreatments.…”

Section: Introductionmentioning

confidence: 99%

Development of a Novel Analytical Method for Determining Trazodone in Human Plasma by Liquid Chromatography Coupled With Mass Spectrometry Coupled With Automatic 2-Dimensional Liquid Chromatograph-Mass Spectrometer Coupler 9500 and Its Application to Therapeutic Drug Monitoring

Ding

Zhang

Cui

et al. 2022

Therapeutic Drug Monitoring

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Background: Trazodone (TZD) is a tetracyclic serotonin antagonist and reuptake inhibitor that is used as a second-generation phenylpiperazine antidepressant. However, the plasma concentrations of TZD have shown individual variations in clinical practice. Quantification of TZD plasma concentrations may be an effective and valuable method to balance the clinical efficacy and adverse reactions. This study aimed to establish a novel liquid chromatography coupled with mass spectrometry (LC-MS) assay for measuring TZD concentrations in human plasma for therapeutic drug monitoring (TDM).Methods: After protein precipitation with acetonitrile, LC-MS quantification of TZD was performed in the multiple reaction monitoring mode with chromatographic separation using a mobile phase of MeOH and 0.1% formic acid in water. This method validation intends to investigate specificity, sensitivity, linearity, precision, accuracy, recovery, matrix effect, and stability according to United states food and drug administration guidelines.Results: This method showed good selectivity because no interfering peaks were observed in the plasma samples during the 2minute run time. The range of the calibration curve was 1-3000 ng/ mL. The detection and quantification limits were 0.3 and 1 ng/mL, respectively. The intraday and interday accuracies were 96.5%-103.4%, with precision relative SD% values of ,5%, except for the limit of quality. The mean TZD recovery from human plasma was 95.4%-104.5%. Finally, this method was successfully applied to TDM in 20 patients. The TZD plasma concentrations of the patients ranged between 21.5 and 2267.3 ng/mL. Conclusions:A novel analytical method was established to measure TZD by LC-MS coupled with an automatic 2-dimensional liquid chromatograph mass spectrometer coupler 9500 (LC-MS/MS-Mate 9500), which is superior to the ordinary LC-MS system in separation, transport, anti-interference, sensitivity, and quantitative analysis stability.

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“…Analytical methods that are reported for the determination of TRH in pharmaceutical formulations include UV absorption measurement at 246 nm 3 ionselective electrode 4, 5 voltammetry 6, 7 and HPLC 2,8 . Various chromatographic methods have been reported for the determination of TRH in biological fluids including HPLC 9,10 , capillary gas chromatography 11 , gas chromatography, mass spectrometry 12 and instrumental thin layer chromatography 13 . Though modern methods of analysis (HPLC, GLC, NMR and Mass) for purity assay of any drug afford simplicity, speed, good specificity and excellent precision and accuracy, they involve sophisticated equipments, which are not in the reach of most laboratories and small-scale industries.…”

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confidence: 99%

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2011

IJPSR

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Spectrophotometric methods have been developed for the assay of trazodone hydrochloride (TRH) in pure and pharmaceutical formulations. These method based on the formation of chloroform soluble ion-association complexes of TRH with bromphenol blue (BPB) and with chlorophenol red (CPR) in buffer of pH 2.0 (for BPB) and buffer of pH of 4 (for CPR) with absorption maximum at 420 nm and at 410 nm for BPB and CPR, respectively. Reaction conditions were optimized to obtain the maximum color intensity. The absorbance was found to increase linearly with increase in concentration of TRH, which was corroborated by the calculated correlation coefficient values (0.9996, 0.9945). The systems obeyed Beer's law in the range of 0.2-14.5 and 0.2-14.1m g/ml for (BPB) and (CPR), respectively. Various analytical parameters have been evaluated and the results have been validated by statistical data. No interference was observed from common excipients present in pharmaceutical formulations. The proposed methods are simple, accurate and suitable for quality control applications. INTRODUCTION: Trazodone hydrochloride, 2-{3-[4-(3chlorophenyl)-1-piperazinyl]propyl}-1, 2, 4-triazolo [4, 3-a]pyridin-3-(2H)-onemonohydrochloride, is a antidepressant. It has been shown to be effective in patients with major depressive disorders and other subsets of depressive disorders. It is generally more useful in depressive disorders associated with insomnia and anxiety. This drug does not aggravate psychotic symptoms in patients with schizophrenia or schizoaffective disorders.

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Measurement of trazodone in plasma and brain of rat by capillary gas chromatography with a nitrogen-selective detector

Cited by 11 publications

References 10 publications

High throughput and sensitive determination of trazodone and its primary metabolite, m-chlorophenylpiperazine, in human plasma by liquid chromatography–tandem mass spectrometry

High throughput and sensitive determination of trazodone and its primary metabolite, m-chlorophenylpiperazine, in human plasma by liquid chromatography–tandem mass spectrometry

Development of a Novel Analytical Method for Determining Trazodone in Human Plasma by Liquid Chromatography Coupled With Mass Spectrometry Coupled With Automatic 2-Dimensional Liquid Chromatograph-Mass Spectrometer Coupler 9500 and Its Application to Therapeutic Drug Monitoring

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