Measurement of tryptic activity in intestinal juice as a diagnostic test of pancreatic disease.

Cook, H. B.; Lennard-Jones, J E; Sherif, S M; Wiggins, H. S.

doi:10.1136/gut.8.4.408

Cited by 52 publications

(43 citation statements)

References 8 publications

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“…Analysis of the results showed that chronic or recurrent pancreatitis and carcinoma of the head of the pancreas were associated with extremely depressed peak trypsin levels (more than 3 sd below the normal mean in six out of eight and three out of three patients respectively). These findings support the original conclusions of Lundh (1962) and of subsequent workers (Cook et al, 1967;McCarthy and Brown, 1969).…”

Section: Discussionsupporting

confidence: 91%

“…The technique of pancreatic stimulation and sampling using a standard test meal was essentially that described by Cook et al (1967). The patients were studied after fasting overnight.…”

Section: Clinical Methodsmentioning

confidence: 99%

“…The threshold of the routine assay, when carried out as described, was equivalent to 50 gg of the trypsin preparation per ml. Cook et al(1967). Table II shows the P values (Student's t) for the comparison between the two sets of means, assuming that they are assessments of the same normal values.…”

Section: Linearity Of Trypsin Assaymentioning

confidence: 99%

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Factors influencing duodenal trypsin levels following a standard test meal as a test of pancreatic function

Zeitlin¹,

Sircus²

1974

Gut

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SUMMARY In this investigation, the measurement of trypsin levels in duodenal juice following a standard test meal (Lundh test) was evaluated as a test of pancreatic function, and a study was made of diseases and other factors which may influence its diagnostic efficiency. The method of trypsin assay, which required only basic laboratory equipment, gave a linear concentration-activity curve, with a threshold at 50 jig of crystalline trypsin per ml. Intestinal juice could be frozen and stored for up to six weeks with no detectable loss of tryptic activity.The normal control values were very similar to those found by other workers and were unaffected by the sex or age of the subject. When used to assess 32 patients in whom the presence or absence of pancreatic disease had been clearly established, the test had a diagnostic success rate of 9400.Retrospective analysis of results from 98 patients showed that trypsin levels were generally grossly reduced in patients with chronic pancreatitis or carcinoma of the head of the pancreas. Trypsin levels were normal in most patients with steatorrhoea not of pancreatic origin. Levels were generally depressed to intermediate levels in patients with a diabetic glucose tolerance, with or without steatorrhoea, but no other sign of pancreatic insufficiency. Some lowering of trypsin levels was also noted in patients having an obstruction of the common bile duct. A small but significant depression of tryptic activity was noted in patients with villous atrophy and no pancreatic disease.

show abstract

Section: Discussionsupporting

confidence: 91%

“…The technique of pancreatic stimulation and sampling using a standard test meal was essentially that described by Cook et al (1967). The patients were studied after fasting overnight.…”

Section: Clinical Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Factors influencing duodenal trypsin levels following a standard test meal as a test of pancreatic function

Zeitlin¹,

Sircus²

1974

Gut

View full text Add to dashboard Cite

show abstract

“…The range of values found in the test is shown in Table 4. On the whole there is good discrimination between normal and diseased glands (Cook et al, 1967;McCarthy and Brown, 1969 (Marks and Bank, 1963). Another such test is the estimation of faecal chymotrypsin and this can be performed on out-patients by an analysis of two stool samples per patient (Amman et al, 1968).…”

Section: Pancreatic Function Testsmentioning

confidence: 99%

Gastric and Pancreatic Function Tests

Bouchier

1970

Ann Clin Biochem

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“…Tryptic activity, whose lower limit of normal is 9.6micro-equiv. min./2 h., is measured using a synthetic substrate and an auto-titrator (Cook, Lennard-Jones, Sherif, and Wiggins, 1967).…”

Section: Investigation Ofpancreatic Functionmentioning

confidence: 99%

Pancreatic Function Tests

Baron

1969

Ann Clin Biochem

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Measurement of tryptic activity in intestinal juice as a diagnostic test of pancreatic disease.

Cited by 52 publications

References 8 publications

Factors influencing duodenal trypsin levels following a standard test meal as a test of pancreatic function

Factors influencing duodenal trypsin levels following a standard test meal as a test of pancreatic function

Gastric and Pancreatic Function Tests

Pancreatic Function Tests

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