Measurement of vitamin A and vitamin E in human serum by high-performance liquid chromatography

Driskell, William J.; Neese, Jane W.; Bryant, Clyde C.; Bashor, Mark M.

doi:10.1016/s0378-4347(00)81869-1

Cited by 271 publications

(83 citation statements)

References 7 publications

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“…Insulin concentrations were determined with an immunoradiometric assay (IRMA assay kit, Biosource Europe, S.A., Nivelles, Belgium). Serum vitamin A and E were determined by high-pressure liquid chromatography (HPLC; Driskell et al, 1982). An enzymatic colorimetric method was used for the determination of cholesterol (Boehringer-Mannheim, GmbH, Mannheim, Germany).…”

Section: Methodsmentioning

confidence: 99%

Therapeutic effects of psyllium in type 2 diabetic patients

et al. 2002

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Objective: The aim of this study was to evaluate the effects of psyllium in type 2 diabetic patients. Design: The study included three phases: phase 1 (1 week), phase 2 (treatment, 14 g fibre=day, 6 weeks) and phase 3 (4 weeks). At the end of each phase a clinical evaluation was performed after the ingestion of a test breakfast of 1824.2 kJ (436 kcal). Measurements included concentrations of blood glucose, insulin, fructosamine, GHbA 1c , C-peptide and 24 h urinary glucose excretion. In addition, uric acid, cholesterol and several mineral and vitamin concentrations were also evaluated. Setting: The study was performed at the Department of Pharmacology, Toxicology and Nursing at the University of Leó n (Spain). Subjects: Twenty type 2 diabetic patients (12 men and 8 women) participated in the study with a mean age of 67.4 y for men and 66 y for women. The mean body mass index of men was 28.2 kg=m 2 and that of women 25.9 kg=m 2 . Results: Glucose absorption decreased significantly in the presence of psyllium (12.2%); this reduction is not associated with an important change in insulin levels (5%). GHbA 1c , C-peptide and 24 h urinary glucose excretion decreased (3.8, 14.9 and 22.5%, respectively) during the treatment with fibre (no significant differences) as well as fructosamine (10.9%, significant differences). Psyllium also reduced total and LDL cholesterol (7.7 and 9.2%, respectively, significant differences), and uric acid (10%, significant difference). Minerals and vitamins did not show important changes, except sodium that increased significantly after psyllium administration. Conclusions:The results obtained indicate a beneficial therapeutic effect of psyllium (Plantaben 1 ) in the metabolic control of type 2 diabetics as well as in lowering the risk of coronary heart disease. We also conclude that consumption of this fibre does not adversely affect either mineral or vitamin A and E concentrations. Finally, for a greater effectiveness, psyllium treatment should be individually evaluated.

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Section: Methodsmentioning

confidence: 99%

Therapeutic effects of psyllium in type 2 diabetic patients

et al. 2002

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“…The KLH titre values were log transformed (Base 2) before analysis. Quantitation of vitamin A in blood plasma (Driskell et al, 1982) and milk (Batra and Hidirogiou, 1995) (Bouda et al, 1980;Bardos et al, 1991 The increase at 3 days (> 0.274 pg/mL plasma) was probably related to the high intake of vitamin A through the colostrum and intestinal absorption of this vitamin (Perthes et al, 1985;bzpinar et al, 1988 According to Flachowsky et al (1990), the level of plasma vitamin A which is not reflective of that in the liver is unsuitable for estimation of the vitamin A status of calves and this is because plasma retinol concentration is homeostatistically regulated (Donoghue and Kronfeld, 1983). Friedman and Sklan (1989) Block and Farmer (1987 (Mitchell, 1967).…”

Section: Methodsmentioning

confidence: 99%

Effect of oral supplements of vitamin A on the plasma retinol levels in calves and their immunological unresponsiveness

Hidiroglou¹,

Markham²

1996

Reprod. Nutr. Dev.

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“…Measurement of α-tocopherol was performed as described by Driskell et al [29] and Miller et al [30]. All procedures were performed under subdued lighting.…”

Section: Selection Of Cases and Control Subjectsmentioning

confidence: 99%

The prospective association between adiponectin and coronary artery disease among individuals with type 1 diabetes. The Pittsburgh Epidemiology of Diabetes Complications Study

et al. 2004

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Aims/hypothesis: Recent findings suggest the potential involvement of adiponectin in obesity, diabetes and cardiovascular disease. We assessed the prospective association between adiponectin concentration and coronary artery disease in individuals with type 1 diabetes. Methods: Participants were identified from the Pittsburgh Epidemiology of Diabetes Complications cohort, a prospective follow-up study of childhood-onset type 1 diabetes. At baseline, subjects had a mean age of 28 years, and a mean diabetes duration of 19 years. Cases (deter mined by physician-diagnosed angina, confirmed myocardial infraction, stenosis ≥50%, ischemic ECG or revascularization) were matched to the control subjects with respect to sex, age and diabetes duration. Samples and risk factors for analyses were identified from the earliest exam prior to incidence in cases. Sera and information on all covariates were available for 28 cases and 34 control subjects. Proportional hazards models were constructed including matching variables. Results: Compared with those in men, adiponectin concentrations were elevated in females (p=0.009) and among individuals with macroalbuminuria (p=0.04). In multivariable analyses (adjusting for standard risk factors as well as lipoprotein measurements determined by nuclear magnetic resonance spectroscopy, E-selectin or antioxidants), adiponectin inversely predicted the incidence of coronary artery disease (hazard ratio=0.37 per 1 SD increase, 95% CI 0.19-0.73, p=0.004). Conclusions/interpretation: The results suggest that increased adiponectin concentration is prospectively associated with a lower risk of coronary artery disease type 1 diabetes. The potential of adiponectin determination as a useful marker of, and potential therapeutic target for, coronary artery disease prevention in type 1 diabetes should be further explored.

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Measurement of vitamin A and vitamin E in human serum by high-performance liquid chromatography

Cited by 271 publications

References 7 publications

Therapeutic effects of psyllium in type 2 diabetic patients

Therapeutic effects of psyllium in type 2 diabetic patients

Effect of oral supplements of vitamin A on the plasma retinol levels in calves and their immunological unresponsiveness

The prospective association between adiponectin and coronary artery disease among individuals with type 1 diabetes. The Pittsburgh Epidemiology of Diabetes Complications Study

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