Measurement of water filtration in skeletal muscle in man by an osmotic transient method

Palm, T.; Nielsen, Søren; Lassen, Niels A.

doi:10.1111/j.1475-097x.1983.tb00851.x

Cited by 3 publications

(3 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The validity and the assumptions basic to the present technique of measuring capillary filtration capacity has been discussed in a previous paper by the authors (Palm, et al, 1983), and will not be discussed in detail here. Emptying of the venous vascular bed with decrease in the venous dispersion factor might be expected when muscle contractions are initiated.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Vascular recruitment in forearm muscles during exercise

Palm

Nielsen

Lassen

1983

Clinical Physiology

Self Cite

View full text Add to dashboard Cite

Blood flow and filtration of water across the vascular bed in human forearm muscles were studied at rest and during graded exercise with a hand ergometer. Blood flow was measured by dye dilution and water filtration was determined after injection of hyperoncotic albumin solution (23%) in the brachial artery creating a tissue to blood ultrafiltration measureable as a dilution in the effluent blood. The filtration constants were expressed as a filtration coefficient Fc (ml water/ml plasma mmHg increase in oncotic pressure), and, multiplying by the plasma flow, as a filtration capacity Kf (ml water/100 ml tissue min mmHg increase in oncotic pressure). During the increase in plasma flow induced by exercise, Fc remained constant at about 0.0007, but Kf increased in parallel with the hyperaemia from 0.0031 to 0.038 when plasma flow increased from 4 to 48 ml (100 ml min)-1. The data suggest a more massive recruitment of exchange area during exercise (a factor 12) than suspected on the basis of ultrafiltration in animals made with the prolonged venous stasis technique (showing a factor 2-5). The estimated variability in exchange surface area indicates, that animal studies of muscle circulation, whether pertaining to capillary permeability or capillary filtration should gain by an independent estimate of the number of capillaries that are flowing during the particular experimental situation.

show abstract

Section: Discussionmentioning

confidence: 99%

“…Multiplication of F, by the plasma flow gave the filtration capacity Kf (ml water/ 100 ml tissue min mmHg). A more detailed description of the technical procedure and the calculation has been given previously (Palm, 1983).…”

Section: Methodsmentioning

confidence: 99%

Vascular recruitment in forearm muscles during exercise

Palm

Nielsen

Lassen

1983

Clinical Physiology

Self Cite

View full text Add to dashboard Cite

show abstract

“…Also, CFC values in skeletal muscle during non-vasodilated conditions seem to be reduced to the same extent (e.g. Cobbold et al 1963, Korthuis et al 1982, 1984 or even more in man (Mellander & Oberg 1967, Palm et al 1983). The average reflection coefficients for albumin, of 0.92 during both situations, in the present study are in excellent agreement with those obtained by lymph protein flux analyses in skeletal muscle with intact circulation (cf.…”

Section: Albumin Clearance During Maximal Vasolidation Following Na-imentioning

confidence: 93%

Transcapillary clearance of albumin in rat skeletal muscle monitored by external detection. Effects of alterations in capillary surface area

Haraldsson

Rippe

1988

Acta Physiologica Scandinavica

View full text Add to dashboard Cite

The effects of noradrenaline (NA)-induced vasoconstriction on the transcapillary passage of albumin was evaluated by an external detection technique, allowing repetitive measurements of albumin clearance (Cl) during various conditions (in the same animal). Six isolated rat hindquarters were perfused with serum-albumin solutions during maximal vasodilation (papaverine 90 microM) and Cl was determined at different net filtration rates (Fv) induced by elevations of venous pressure. Then, the perfusate was changed to one of similar composition but containing noradrenaline (2-4 microM), and the procedure of determining Cl vs. Fv was repeated. Tissue accumulation of [99mTc]albumin was expressed in terms of clearance, using the isogravimetric Cl of defined muscle samples during maximum vasodilation in separate experiments as reference, the latter being 0.0246 +/- 0.0012 ml min-1 per 100 g. Noradrenaline caused an increase in vascular resistance from 2 to 14 mmHg min 100 g ml-1, while the Cl vs. Fv relationship was shifted downwards in a fashion parallel with the control Cl vs. Fv curve. For Fv = 0, Cl was 0.0101 +/- 0.0014 ml min-1 per 100 g during NA challenge. The average reflection coefficient for albumin (sigma tot) was 0.92 +/- 0.01 irrespective of vascular tone. Thus, both albumin clearance and the capillary filtration coefficient (CFC) seem to vary in direct proportion to the capillary surface area available for exchange.

show abstract

Comments to the recent papers by Palm, Nielsen and Lassen concerning measurements of filtration capacity

Folkow

1984

Clinical Physiology

View full text Add to dashboard Cite

Measurement of water filtration in skeletal muscle in man by an osmotic transient method

Cited by 3 publications

References 17 publications

Vascular recruitment in forearm muscles during exercise

Vascular recruitment in forearm muscles during exercise

Transcapillary clearance of albumin in rat skeletal muscle monitored by external detection. Effects of alterations in capillary surface area

Comments to the recent papers by Palm, Nielsen and Lassen concerning measurements of filtration capacity

Contact Info

Product

Resources

About