Measurements of acoustic radiation force of ultrahigh frequency ultrasonic transducers using model-based approach

Abstract

Even though ultrahigh frequency ultrasonic transducers over 60 MHz have been used for single-cell-level manipulation such as intracellular delivery, acoustic tweezers, and stimulation to investigate cell phenotype and cell mechanics, no techniques have been available to measure the actual acoustic radiation force (ARF) applied to target cells. Therefore, we have developed an approach to measure the ARF of ultrahigh frequency ultrasonic transducers using a theoretical model of the dynamics of a solid sphere in a gelatin phantom. To estimate ARF at the focus of a 130 MHz transducer, we matched measured maximum displacements of a solid sphere with theoretical calculations. We selected appropriate ranges of input voltages and pulse durations for single-cell applications, and the estimated ARF was in the range of tens of μ N. To gauge the influence of pulse duration, an impulse of different pulse durations was estimated. Fluorescence resonance energy transfer live cell imaging was demonstrated to visualize calcium transport between cells after a target single cell was stimulated by the developed ultrasonic transducer.

Effects of low intensity pulsed ultrasound stimulation on metabolic lipolysis of adipocytes

“…Three-axis computer-controlled position system (ILS150CC, Newport Corp., USA) was used to scan 30 mm by 30 mm area to stimulate 3T3-L1 cells in a 35 mm dish (Fig. 1a, b) [24, 25]. A custom LabView (National Instruments, USA) computer program was used to control the positioning system to repeatedly scan the same area seven times for 10 minutes.…”

“…The acoustic output such as acoustic pressure and spatial peak temporal average intensity ( I SPTA ) of the 2 MHz ultrasonic transducer was measured with a needle hydrophone (HGL-0085, Onda) using the same three-axis positioning system [25, 26].…”

Landscape of Cellular Bioeffects Triggered by Ultrasound-Induced Sonoporation

“…Other possibilities open when the target cells are close to the source of US. In such a case, bulk acoustic waves can be focused in a small area to generate concentrated acoustic energy [ 48 , 49 , 50 , 51 ], or hyperfrequency bulk acoustic waves can be used to induce acoustic streaming and create pores in the cell membrane [ 52 , 53 ].…”

Modeling cancer metastasis using acoustically bio-printed patient-derived 3D tumor microtissues

“…The acoustic radiation force has excellent biocompatibility and is well suited for biological applications. [32][33][34] The acoustic 3D printing device, due to its acoustically driven design and opencavity structure, can achieve fine manipulation of micro-liquids and non-blocking printing of high-viscosity materials. [35][36][37][38][39] We combined patient-derived cells and microtissue culture technology to construct an invasion model for colorectal tumor microtissue-CAFs, studying the migration mechanisms of the tumors and the CAFs.…”