Measurements of amino acid transport in internally dialyzed giant axons

Horn, L.W.

doi:10.1007/bf01869714

Cited by 4 publications

(2 citation statements)

References 26 publications

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“…It is not known what role glycine and glutathione have in the cephalopod olfactory system. Glycine acts at several synapses of the squid central nervous system (Caldwell and Lea, 1978; Horn, 1986; Vinogradova et al, 2002; Lima et al, 2003), but, because there is no clear role for glycine in our model organisms, we cannot draw any conclusions about the role of glycine in squid. There were two classes of cells in squid OE that had high glutathione labeling (Classes 5 and 6), and were morphologically identified as a subset of type 2 cells.…”

Section: Discussionmentioning

confidence: 96%

Cross‐Species Comparison of Metabolite Profiles in Chemosensory Epithelia: An Indication of Metabolite Roles in Chemosensory Cells

Mobley

Lucero

Michel

2008

The Anatomical Record

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Comparative studies of chemosensory systems in vertebrates and invertebrates have greatly enhanced our understanding of anatomical and physiological constraints of chemical detection. Immunohistochemical comparisons of chemosensory systems are difficult to make across species due to limited cross-reactivity of mammalian-based antibodies. Immunostaining chemosensory tissues with glutaraldehyde-based antibodies generated against small metabolites in combination with hierarchical cluster analyses provide a novel approach for identifying and classifying cell types regardless of species. We used this ''metabolite profiling'' technique to determine whether metabolite profiles can be used to identify cell classes within and across different species including mouse, zebrafish, lobster and squid. Within a species, metabolite profiles for distinct cell classes were generally consistent. We found several metabolite-based cell classifications that mirrored function or receptor protein-based classifications. Although profiles of all six metabolites differed across species, we found that specific metabolites were associated with certain cell types. For example, elevated levels of glutathione were characteristic of nonsensory cells from vertebrates, suggesting an antioxidative role in non-neuronal cells in sensory tissues. Collectively, we found significantly different metabolite profiles for distinct cell populations in chemosensory tissue within all of the species studied. Based on their roles in other systems or cells, we discuss the roles of L-arginine, L-aspartate, L-glutamate, glycine, glutathione, and taurine within chemosensory epithelia. Anat Rec, 291:410-432, 2008. 2008 Key words: olfactory; vomeronasal; mouse; zebrafish; lobster; squidAbbreviations used: AOI = area of interest; Arg = arginine; Asp = aspartate; ATG = aesthetasc tegumental glands; EC = ensheathing cell; Glu = glutamate; GSH = glutathione; Gly = glycine; IR = immunoreactivity; NOS = nitric oxide synthase; OE = olfactory epithelium; ORN = olfactory receptor neuron; RGB = red, green, blue; SC = sustentacular cell; Tau = taurine; VN = vomeronasal neuron; VNO = vomeronasal organ.The Supplementary Material referred to in this article can be found at

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Section: Discussionmentioning

confidence: 96%

Cross‐Species Comparison of Metabolite Profiles in Chemosensory Epithelia: An Indication of Metabolite Roles in Chemosensory Cells

Mobley

Lucero

Michel

2008

The Anatomical Record

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“…Recently, Horn (1986) has developed a modification using a much more permeable dialysis tubing. For the present study, we have used a dialysis tubing with a nominal molecular-weight cut-off of 6,000 (Spectrapor HF MWCO 6000, Spectrum, Los Angeles, CA).…”

Section: Internal Dialysismentioning

confidence: 99%

The interaction of intracellular Mg2+ and pH on Cl- fluxes associated with intracellular pH regulation in barnacle muscle fibers.

Russell

Brodwick

1988

The Journal of General Physiology

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The intraceilular dialysis technique was used to measure unidirectional C1-fluxes and net acid extrusion by single muscle fibers from the giant barnacle. Decreasing pH~ below normal levels of 7.35 stimulated both CI-effiux and influx. These increases of CI-fluxes were blocked by disulfonic acid stilbene derivatives such as SITS and DIDS. The SITS-sensitive C1-effiux was sharply dependent upon pHi, increasing ~20-fold as pHi was decreased from 7.35 to 6.7. Under conditions of normal intracellular Mg ')+ concentration, the apparent pK~ for the activation of C1-effiux was 7.0. We found that raising [MgZ+]i, but not [Mg'2+],,, had a pronounced inhibitory effect on both SITS-sensitive unidirectional C1-fluxes as well as on SITS-sensitive net acid extrusion. Increasing [Mg')+]~ shifted the apparent pKg. of CIefflux to a more acid value without affecting the maximal flux that could be attained. This relation between pHi and [Mg'~+]i on SITS-sensitive CI-effiux is consistent with a competition between H ions and Mg ions. We conclude that the SITS-inhibitable C1-fluxes are mediated by the pHi-regulatory transport mechanism and that changes of intracellular Mg 2+ levels can modify the activity of the pHi regulator/anion transporter.

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Intracellular Ca2+ does not activate the SITS-sensitive anion transporter in barnacle muscle

Matsumoto

Russell

1988

Biochimica et Biophysica Acta (BBA) - Biomembranes

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Measurements of amino acid transport in internally dialyzed giant axons

Cited by 4 publications

References 26 publications

Cross‐Species Comparison of Metabolite Profiles in Chemosensory Epithelia: An Indication of Metabolite Roles in Chemosensory Cells

Cross‐Species Comparison of Metabolite Profiles in Chemosensory Epithelia: An Indication of Metabolite Roles in Chemosensory Cells

The interaction of intracellular Mg2+ and pH on Cl- fluxes associated with intracellular pH regulation in barnacle muscle fibers.

Intracellular Ca2+ does not activate the SITS-sensitive anion transporter in barnacle muscle

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