Measurements of Chlorpyrifos Levels in Forager Bees and Comparison with Levels that Disrupt Honey Bee Odor-Mediated Learning Under Laboratory Conditions

Urlacher, Elodie; Monchanin, Coline; Rivière, Coraline; Richard, Freddie-Jeanne; Lombardi, Christie; Michelsen-Heath, Sue; Hageman, Kimberly J.; Mercer, Alison R.

doi:10.1007/s10886-016-0672-4

Cited by 58 publications

(32 citation statements)

References 64 publications

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“…The potential off-target health consequences of organophosphate pesticide exposure for humans include neonatal developmental abnormalities (4,5), endocrine disruption (6), neurodegeneration, cancer (7,8), metabolic disruption (9), heart disease (10), chronic kidney disease, and other less common pathologies. In addition, organophosphate pesticides are reported to have negative impacts on honeybee colonies, which are critical pollinators for numerous agricultural products (11)(12)(13). Although the evidence is mixed and often correlative, the original use of these organophosphates as nerve agents strongly suggests that these pesticides are not aligned with human or wildlife health.…”

mentioning

confidence: 99%

Probiotic Lactobacillus rhamnosus Reduces Organophosphate Pesticide Absorption and Toxicity to Drosophila melanogaster

Trinder

McDowell

Daisley

et al. 2016

Appl Environ Microbiol

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Organophosphate pesticides used in agriculture can pose health risks to humans and wildlife. We hypothesized that dietary supplementation with Lactobacillus, a genus of commensal bacteria, would reduce absorption and toxicity of consumed organophosphate pesticides (parathion and chlorpyrifos [CP]). Several Lactobacillus species were screened for toleration of 100 ppm of CP or parathion in MRS broth based on 24-h growth curves. Certain Lactobacillus strains were unable to reach stationary-phase culture maxima and displayed an abnormal culture morphology in response to pesticide. Further characterization of commonly used, pesticide-tolerant and pesticide-susceptible, probiotic Lactobacillus rhamnosus strain GG (LGG) and L. rhamnosus strain GR-1 (LGR-1), respectively, revealed that both strains could significantly sequester organophosphate pesticides from solution after 24-h coincubations. This effect was independent of metabolic activity, as L. rhamnosus GG did not hydrolyze CP and no difference in organophosphate sequestration was observed between live and heat-killed strains. Furthermore, LGR-1 and LGG reduced the absorption of 100 M parathion or CP in a Caco-2 Transwell model of the small intestine epithelium. To determine the effect of sequestration on acute toxicity, newly eclosed Drosophila melanogaster flies were exposed to food containing 10 M CP with or without supplementation with live LGG. Supplementation with LGG simultaneously, but not with administration of CP 3 days prior (prophylactically), mitigated CP-induced mortality. In summary, the results suggest that L. rhamnosus may be useful for reducing toxic organophosphate pesticide exposure via passive binding. These findings could be transferable to clinical and livestock applications due to affordability and practical ability to supplement products with food-grade bacteria. IMPORTANCEThe consequences of environmental pesticide pollution due to widespread usage in agriculture and soil leaching are becoming a major societal concern. Although the long-term effects of low-dose pesticide exposure for humans and wildlife remain largely unknown, logic suggests that these chemicals are not aligned with ecosystem health. This observation is most strongly supported by the agricultural losses associated with honeybee population declines, known as colony collapse disorder, in which pesticide usage is a likely trigger. Lactobacilli are bacteria used as beneficial microorganisms in fermented foods and have shown potentials to sequester and degrade environmental toxins. This study demonstrated that commonly used probiotic strains of lactobacilli could sequester, but not metabolize, organophosphate pesticides (parathion and chlorpyrifos). This Lactobacillus-mediated sequestration was associated with decreased intestinal absorption and insect toxicity in appropriate models. These findings hold promise for supplementing human, livestock, or apiary foods with probiotic microorganisms to reduce organophosphate pesticide exposure.O rganophosphate pesticides are ...

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mentioning

confidence: 99%

Probiotic Lactobacillus rhamnosus Reduces Organophosphate Pesticide Absorption and Toxicity to Drosophila melanogaster

Trinder

McDowell

Daisley

et al. 2016

Appl Environ Microbiol

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“…Besides its acute lethal effects, chlorpyrifos severely affected the formation and retrieval of appetitive memory in the honeybee, which could have an impact on the foraging behavior and honey production (Urlacher et al 2016). Eighty percent of dead honeybee samples collected periodically from four different locations during the citrus and stone fruit tree blooming season in the Valencian Community (Spain) had chlorpyrifos (Calatayud-Vernich et al 2016).…”

Section: Side Effects On Pollinatorsmentioning

confidence: 99%

Vine and citrus mealybug pest control based on synthetic chemicals. A review

Mansour¹,

Belzunces²,

Suma³

et al. 2018

Agron. Sustain. Dev.

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Synthetic chemicals are extensively used to limit the substantial crop damage induced by two closely related scale insects, the vine mealybug Planococcus ficus (Signoret) and the citrus mealybug Planococcus citri Risso (Hemiptera: Pseudococcidae). Both organisms are economically important pests occurring in vineyards and/or in citrus orchards worldwide. Synthetic chemicals can be either incorporated in pesticides aimed at directly controlling these pests or used as semiochemicals (i.e., sex pheromones) for monitoring, mass trapping, mating disruption, and/or for kairomonal attraction to enhance parasitoid performances. Growing evidence of both an alarming bee decline and destruction of auxiliary fauna driven by pesticides have stimulated an urgent need for in-depth research clarifying the adverse side effects of pesticides on beneficial arthropods. We have reviewed the current knowledge on mealybug pest control based on insecticides and semiochemicals. We highlight the following major advances: (1) How the active substances of insecticides (four organophosphates, imidacloprid, buprofezin, and spirotetramat) affect target and non-target organisms, (2) in which contexts and how a semiochemical-based strategy could be applied to deal with serious mealybug infestations, and (3) the implications of the appropriate exploitation of these synthetic chemicals for sustainable development. Using selective insecticides with novel modes of action and long-lasting efficacy in combination with eco-friendly semiochemical-based tools is a promising strategy for developing sustainable integrated pest management programs. This would help to maintain biodiversity dynamics and vital ecosystem services, thereby sustaining crop yields.

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“…They are predominately used to control pests in agricultural settings. Human exposure to OPs can adversely affect human health (Liu et al 2014;Lerro et al 2015;Sánchez-Santed et al 2016;Urlacher et al 2016;Muñoz-Quezada et al 2016a). Acute intoxication results in headache and abdominal pain, nausea, vomiting, dizziness, dermatitis, skin lesions, sleep problems, salivation, blurry vision, leg cramps or muscular weakness (Azazh 2011;Jensen et al 2011;Suratman et al 2015), extrapyramidal symptoms (Reji et al 2016), kidney injury (Lee et al 2015), among others.…”

Section: Introductionmentioning

confidence: 99%

Reliability and factorial validity of a questionnaire to assess organophosphate pesticide exposure to agricultural workers in Maule, Chile

Muñoz-Quezada

Lucero

Bradman

et al. 2018

International Journal of Environmental Health Research

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The aim was to evaluate the reliability and validity of a questionnaire to assess organophosphate pesticide (OP) exposure in agricultural workers. We then enrolled a random sample of 114 agricultural workers from the region of Maule, Chile (mean age = 50 years [SD = 12]). An internal consistency analysis (Cronbach's alpha> 0.70) and a Varimax rotational factorial analysis were applied. The instrument had a high reliability to predict likely occupational pesticide exposures: Cronbach's alpha = 0.95, the Kaiser-Meyer-Olkin (KMO) measure was 0.90 and the Bartell sphericity test = p < 0.001. Four factors explaining 68% of the variance were extracted. The factors identified were as follows: (1) labor conditions during application of OPs; (2) use of personal protective equipment; (3) workplace conditions related to OP exposure and (4) home conditions related to OP exposure. The questionnaire has adequate metric properties to characterize likely OP exposure of agricultural workers and to explore associated working and home conditions.

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Measurements of Chlorpyrifos Levels in Forager Bees and Comparison with Levels that Disrupt Honey Bee Odor-Mediated Learning Under Laboratory Conditions

Cited by 58 publications

References 64 publications

Probiotic Lactobacillus rhamnosus Reduces Organophosphate Pesticide Absorption and Toxicity to Drosophila melanogaster

Probiotic Lactobacillus rhamnosus Reduces Organophosphate Pesticide Absorption and Toxicity to Drosophila melanogaster

Vine and citrus mealybug pest control based on synthetic chemicals. A review

Reliability and factorial validity of a questionnaire to assess organophosphate pesticide exposure to agricultural workers in Maule, Chile

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