Measurements of genotoxic potential of cadmium in different tissues of fresh water climbing perch Anabas testudineus (Bloch), using the comet assay

Ahmed, Md. Kawser; Parvin, Elora; Arif, Murtaza; Akter, Mosammat Salma; Khan, Mohammad Rezwan; Islam, Monirul

doi:10.1016/j.etap.2010.04.003

Cited by 18 publications

(8 citation statements)

References 36 publications

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“…More than 50% TDNA as found in liver and kidney tissue at higher (2 mg/L) concentration of lead chloride might have caused apoptotic cell death of exposed fish. A recent study of Ahmed et al (2010b) reported higher % Tail DNA (38.81 ± 0.55%) in liver tissue of A. testudineus (Bloch) at 2 mg/L concentration of cadmium exposure. Exposure of same fish to lead and cadmium at same concentration (2 mg/L) reveals that lead is more genotoxic (57.84 ± 0.27% Tail DNA) than the cadmium.…”

Section: Discussionmentioning

confidence: 95%

Genetic damage induced by lead chloride in different tissues of fresh water climbing perch Anabas testudineus (Bloch)

Ahmed

Parvin

Arif

et al. 2011

Environ Monit Assess

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The present investigation was undertaken to study the induction of DNA damage by lead chloride (PbCl(2)) in freshwater climbing perch Anabas testudineus using alkaline single cell gel electrophoresis (comet assay). Based on the LC(50) values of lead chloride of A. testudineus three different concentrations viz., 0.1, 1.0 and 2.0 mg/L were selected to expose fish. The DNA damage was observed in the gill, kidney and liver tissue as the percentage of DNA in comet tails and comet heads in the tissue of the exposed fish. DNA damage at different concentrations showed sensitivity to particular tissue. The liver tissue exhibited significantly (p < 0.01) higher DNA damage, followed by kidney and gill. However, the DNA damage was found to be dose dependent; at 2 mg/L of PbCl(2) the tail and head DNA of liver tissue were 57.84% and 39.49%, in kidney tissue the values were 52.36% and 44.97% whereas in gill tissue the values were 48.86% and 48.96% respectively. The current study explored the utility of the comet assay for in vivo laboratory studies using A. testudineus species for screening the genotoxic potential of lead chloride.

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Section: Discussionmentioning

confidence: 95%

Genetic damage induced by lead chloride in different tissues of fresh water climbing perch Anabas testudineus (Bloch)

Ahmed

Parvin

Arif

et al. 2011

Environ Monit Assess

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“…Chandra and Khuda‐bukhsh () showed the induction of genotoxic effects of CdCl 2 singly and also in combination with azadirachtin (Aza) on a fish, Oreochromis mossambicus . Ahmed and coworkers (Ahmed et al, ) measured the induction of DNA damage by CdCl 2 in freshwater climbing perch Anabas testudineus (Bloch) using the alkaline single cell gel electrophoresis (comet assay). They determined the extent of DNA damage in gill, kidney, and liver of the organisms as the percentage of DNA in comet tails and comet heads in the tissue of the fish specimens exposed to 0.1, 1.0, and 2.0 mg L −1 concentrations of CdCl 2 .…”

Section: Discussionmentioning

confidence: 99%

“…In cultured cells, cadmium produces direct and indirect genotoxic effects such as DNA strand breaks, DNA–protein cross‐links, oxidative DNA damage, and chromosomal aberrations (Dally and Hartwig, ; Hwua and Yang, ; Misra et al, ). Recent works have demonstrated the interest of using the comet assay for assessing the genotoxicity of cadmium in aquatic ecosystems (Faverney et al, ; Hook and Lee, ; Emmanouil et al, ; Chang et al, ; Ahmed et al, ).…”

Section: Introductionmentioning

confidence: 99%

Genotoxicity of cadmium chloride in the marine gastropod Nerita chamaeleon using comet assay and alkaline unwinding assay

Sarkar

Bhagat

Ingole

et al. 2013

Environmental Toxicology

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This paper presents an evaluation of the genotoxic effects of cadmium chloride (CdCl2 ) on marine gastropod, Nerita chamaeleon following the technique of comet assay and the DNA alkaline unwinding assay (DAUA). In this study, the extent of DNA damage in gill cells of N. chamaeleon was measured after in vivo exposure to four different concentrations (10, 25, 50, and 75 µg/L) of CdCl2 . In vitro exposure of hydrogen peroxide (H2 O2 ; 1, 10, 25, and 50 µM) of the gill cells showed a significant increase in the percentage tail DNA, Olive tail moment, and tail length (TL). Significant changes in percentage tail DNA by CdCl2 exposure were observed in all exposed groups of snails with respect to those in control. Exposure to 75 µg/L of CdCl2 produced significant decrease in DNA integrity as measured by DAUA at all duration with respect to control. In vivo exposure to different concentrations of CdCl2 (10, 25, 50, and 75 µg/L) to N. chamaeleon showed considerable increase in DNA damage as observed by both alkaline comet assay and the DAUA. The extent of DNA damage in marine gastropods determined by the application of alkaline comet assay and DAUA clearly indicated the genotoxic responses of marine gastropod, N. chamaeleon to a wide range of cadmium concentration in the marine environment.

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“…In addition, it would be indicating that the method for detecting genotoxic effects seems to be more sensitive than the one for detecting accumulation. Several studies using fish (Anabas testudineus, Carassius gibelio, Labeo rohita, Corydoras paleatus, Danio rerio) have demonstrated Cd genotoxicity at concentrations ranging from 17 to 2.0 lM (Ahmed et al 2010;Cambier et al 2010;Jindal and Verma 2015), placing again O. bonariensis (gill LOEC 9.4 nM) among the most sensitive one.…”

Section: Resultsmentioning

confidence: 99%

“…Cadmium carcinogenicity has been demonstrated in humans and others mammals (Tchounwou et al 2012). Genotoxic effects have been also reported in several freshwater fish species (Ahmed et al 2010;Jindal and Verma 2015). In addition, Cr ?6 has been classify as human carcinogen acting via oxidative stress-dependent and -independent mechanisms (Nickens et al 2010).…”

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confidence: 92%

Genotoxic Effects Induced by Cd+2, Cr+6, Cu+2 in the Gill and Liver of Odontesthes bonariensis (Piscies, Atherinopsidae)

Gasulla

Picco

Carriquiriborde

et al. 2016

Bull Environ Contam Toxicol

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Genotoxic effects of Cd(+2), Cr(+6), and Cu(+2) on the gill and liver of the Argentinean Silverside (Odontesthes bonariensis) were studied using the comet assay and in relation with the metal tissue accumulation. Fish were exposed to three waterborne concentrations of each metal for 2 and 16 days. Genotoxicity was assessed by the single cell gel electrophoresis (comet assay). After 2 days, significant increase of the genetic damage index (GDI) was only observed in the gill of fish exposed to Cr(+6) and Cu(+2), and the LOECs were 2160 nM and 921.1 nM, respectively. The gill LOEC for Cd(+2) by 16 days was 9.4 nM. In the liver, LOECs were obtained only for Cd(+2) and Cr(+6) and were 9.4 and 2160 nM, respectively. The three metals were able to induce genotoxic effects at environmentally relevant concentrations and the gill was the most sensitive organ.

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Measurements of genotoxic potential of cadmium in different tissues of fresh water climbing perch Anabas testudineus (Bloch), using the comet assay

Cited by 18 publications

References 36 publications

Genetic damage induced by lead chloride in different tissues of fresh water climbing perch Anabas testudineus (Bloch)

Genetic damage induced by lead chloride in different tissues of fresh water climbing perch Anabas testudineus (Bloch)

Genotoxicity of cadmium chloride in the marine gastropod Nerita chamaeleon using comet assay and alkaline unwinding assay

Genotoxic Effects Induced by Cd+2, Cr+6, Cu+2 in the Gill and Liver of Odontesthes bonariensis (Piscies, Atherinopsidae)

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