This study aims to estimate the radon concentration and annual effective dose rate from water samples in Gezira State, Sudan. The study used 26 samples from various locations and analyzed them using a RAD7 electronic radon detector and RAD-H2O accessory (Durridge Co., USA). The effective dose rates received by individuals were estimated based on ingestion, inhalation, and age group (infants, children, and adults). The radon concentration in water samples ranged from (1.55±0.13 to 18.38±3.05) Bq.l-1, with a mean value of 7.68±1.07 Bq.l-1. The effective dose rates reserved by radon released from water to indoor air ranged from (3.87±0.32 to 45.89±7.62) Sv.y-1 with a mean value of 19.17±2.68 Sv.y-1. The effective dose rates due to ingestion, inhalation, and the total annual effective dose rate were found to range from (3.80±0.32 to 45.03±7.47; 3.91±0.33 to 46.32±7.69 and 7.70±0.65 to 91.35±15.16) Sv.y-1respectively, with mean values of (18.81±2.63; 19.34±2.71 and 38.15±5.34) Sv.y-1 respectively. Meanwhile, the calculated annual effective dose rates for infants, children, and adults have ranges of (16.28±1.37to 192.99±32.03; 10.85±0.91 to 128.66±21.35 and 7.75±0.65 to 91.90±15.25) Sv.y-1 respectively, with mean values of (80.60±11.27; 53.73±7.51and 38.38±5.37) Sv.y-1 respectively. The study shows that 23.1% of the samples exceeded the maximum contamination level, with 76.9% below the USEPA's maximum contaminant level of 11.1 Bq.l-1. All samples (100%) showed radon below the WHO and EU's reference level of 100 Bq.l-1. The overall radiation dose due to radon from water increased with age and water consumption rates, but was significantly lower than the UNSCEAR and WHO recommended limit of 103 Sv.y-1(1mSv.y-1). The radiation dose rate received by the bronchial epithelium via inhalation was about double the value if compared to stomach walls via ingestion. The findings were compared to other studies.