Measurements of the genotoxic potential of (xeno-)oestrogens in the bivalve mollusc Scrobicularia plana, using the Comet assay

Petridis, Panos; Jha, Awadhesh N.; Langston, W.J.

doi:10.1016/j.aquatox.2009.05.010

Cited by 36 publications

(22 citation statements)

References 42 publications

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“…Some environmental estrogenic compounds including BPA, 4-NP, 4-t-OP, and their ethoxylates, have shown mutagenic effects in in vitro or in vivo assays [51][52][53][54][55][56]. Phthalate compounds, including dibutyl phthalate, diisobutyl phthalate, bis (2-ethylhexyl) phthalate, and mono-2-ethylhexyl phthalate, have potential mutagenic and carcinogenic effects on organisms and trick a possible pathway of tumor initiation in animals [57][58][59][60][61].…”

Section: Discussionmentioning

confidence: 99%

Assessment of hormonal activities and genotoxicity of industrial effluents using in vitro bioassays combined with chemical analysis

Fang

Ying

Zhao

et al. 2012

Enviro Toxic and Chemistry

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Wastewaters from various industries are a main source of the contaminants in aquatic environments. The authors evaluated the hormonal activities (estrogenic/anti‐estrogenic activities, androgenic/anti‐androgenic activities) and genotoxicity of various effluents from textile and dyeing plants, electronic and electroplate factories, pulp and paper mills, fine chemical factories, and municipal wastewater treatment plants in the Pearl River Delta region by using in vitro bioassays (yeast estrogen screen [YES]; yeast androgen screen [YAS]; and genotoxicity assay [umu/SOS]) combined with chemical analysis. The results demonstrated the presence of estrogenic, anti‐estrogenic, and anti‐androgenic activity in most industrial effluents, whereas no androgenic activities were detected in all of the effluents. The measured estrogenic activities expressed as estradiol equivalent concentrations (EEQs) ranged from below detection (3 of 26 samples) to 40.7 ng/L, with a mean of 7.33 ng/L in all effluents. A good linear relationship was found between the EEQs measured by YES bioassay and the EEQs calculated from chemical concentrations. These detected estrogenic compounds, such as 4‐nonylphenol and estrone, were responsible for the estrogenic activities in the effluents. The genotoxic effects expressed as benzo[a]pyrene equivalent concentrations (BaP EQs) varied between below detection and 88.2 µg/L, with a mean of 8.76 µg/L in all effluents. The target polycyclic aromatic hydrocarbons were minor contributors to the genotoxicity in the effluents, and some nontarget compounds in the effluents were responsible for the measured genotoxicity. In terms of estrogenic activities and genotoxicity, discharge of these effluents could pose high risks to aquatic organisms in the receiving environments. Environ. Toxicol. Chem. 2012;31:1273–1282. © 2012 SETAC

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Section: Discussionmentioning

confidence: 99%

Assessment of hormonal activities and genotoxicity of industrial effluents using in vitro bioassays combined with chemical analysis

Fang

Ying

Zhao

et al. 2012

Enviro Toxic and Chemistry

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“…comet assay), but may also have potential as biomarkers of effects once their link to higher level detrimental responses is more defined and accepted in line with human or mammalian studies. For example, various studies have demonstrated that there is a dose or concentration-dependent increase in strand breaks (measured by the comet assay) in marine and estuarine invertebrates in response to various environmental contaminants (Alamri et al, 2012;Canty et al, 2009;Cheung et al, 2006a;D'Agata et al, 2013;Jha et al, 2005;Lafarga-De la Cruz et al, 2008;Petridis et al, 2009). However, links between these effects and more ecologically relevant ones are only just starting to be elucidated.…”

Section: Appropriate Selection Of Biomarkersmentioning

confidence: 97%

“…dab and flounder) and in freshwater molluscs exposed to discrete sources of pesticide pollution in other parts of the world, and is now becoming an important indicator of endocrine-disrupting exposure in marine molluscs (Langston et al, 2007). In this context, clams collected from a nearby Plym estuary, exhibited enhanced DNA damage in their haemocytes as determined by comet assay when exposed to a range of known endocrine disrupting agents under laboratory conditions (Petridis et al, 2009). The study indicated that contaminants such as endocrine disrupting agents could manifest their toxicity in a variety of ways.…”

Section: Anthropogenic Inputmentioning

confidence: 98%

Applications of biological tools or biomarkers in aquatic biota: A case study of the Tamar estuary, South West England

Dallas

Jha

2015

Marine Pollution Bulletin

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“…The comet assay is a widely used technique that detects single or double-strand breaks and therewith genotoxic effects of compounds or apoptosis (Tice et al 2000, Liao et al 2009). Studies using the comet assay detected that steroids, in particular estradiol, synthetic (xeno)eostrogens (Yared et al 2002, Djelic & Anderson 2003, Petridis et al 2009) or thyroid hormone and T 3 (Djelic & Anderson 2003), can be genotoxic agents, as they induce the formation of reactive oxygen species. No works have been published so far evaluating the effects of stress hormones on DNA damage in oocytes.…”

Section: Discussionmentioning

confidence: 99%

Morphological and molecular effects of cortisol and ACTH on zebrafish stage I and II follicles

et al. 2015

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Oogenesis in zebrafish (Danio rerio) is controlled by the hypothalamus-pituitary-gonadal axis and reproductive hormones. In addition, an interference of stress hormones is known with reproductive biology. In the presented work, we aimed to explore the hypothesis that cortisol (Cort) and ACTH may affect early oogenesis in zebrafish, given the presence of the specific receptors for glucocorticoids and ACTH in the zebrafish ovary. Follicles at stages I and II were exposed in vitro to 1 mM Cort and ACTH for 48 h, then ultrastructural and molecular effects were analyzed. The comet assay demonstrated increased tail moments for Cort and ACTH treatment indicative of DNA damage. The mRNA expression of apoptotic genes (bax, bcl-2) was not altered by both treatments, but Cort increased significantly the expression of the ACTH receptor (mc2r). Cort stimulated the presence of the endoplasmic reticulum, predominantly at stage II, while ACTH induced a strong vacuolization. Viability of oocytes was not affected by both treatments and fluorescent staining (monodansylcadaverine/acridine orange) indicated a reduced quantity of autophagosomes for ACTH, and lower presence of nucleic acids in ooplasm for Cort and ACTH. Concluding, different responses were observed for stress hormones on early stages of zebrafish oocytes, which suggest a role for both hormones in the stress-mediated adverse effects on female gametogenesis. Reproduction (2015) 150 429-436

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Measurements of the genotoxic potential of (xeno-)oestrogens in the bivalve mollusc Scrobicularia plana, using the Comet assay

Cited by 36 publications

References 42 publications

Assessment of hormonal activities and genotoxicity of industrial effluents using in vitro bioassays combined with chemical analysis

Assessment of hormonal activities and genotoxicity of industrial effluents using in vitro bioassays combined with chemical analysis

Applications of biological tools or biomarkers in aquatic biota: A case study of the Tamar estuary, South West England

Morphological and molecular effects of cortisol and ACTH on zebrafish stage I and II follicles

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