Measurements of trabecular bone structure in man (for radionuclide dosimetry

Beddoe, A H; Darley, P.J.; Spiers, F. W.

doi:10.1088/0031-9155/21/4/010

Cited by 88 publications

(71 citation statements)

References 12 publications

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“…Nevertheless, stereologic arguments would suggest that if sufficient numbers of 2-dimensional distances were taken for the subject specimens and for multiple and sufficiently distant microtome sections of each biopsy specimen, the results from multiple 2-dimensional images should closely approximate their 3D distribution. This assumption is further supported by the fact that the bone trabeculae are isotropically arranged in the os coxae, and thus sampling of different core angles would not be required (19).…”

Section: Discussionmentioning

confidence: 99%

Spatial Distribution of Blood Vessels and CD34+ Hematopoietic Stem and Progenitor Cells Within the Marrow Cavities of Human Cancellous Bone

Watchman¹,

Bourke²,

Lyon³

et al. 2007

Journal of Nuclear Medicine

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Current bone marrow dosimetry methods inherently assume that the target cells of interest for the assessment of leukemia risk (stochastic effects) or marrow toxicity (deterministic effects) are uniformly localized throughout the marrow cavities of cancellous bone. Previous studies on mouse femur, however, have demonstrated a spatial gradient for the hematopoietic stem and progenitor cells, with higher concentrations near the bone surfaces. The objective of the present study was to directly measure the spatial concentration of these cells, as well as marrow vasculature structures, within images of human disease-free bone marrow. Methods: Core-biopsy samples of normal bone marrow from the iliac crest were obtained from clinical cases at Shands Hospital at the University of Florida Department of Pathology. The specimens were sectioned and immunohistochemically stained for CD34 (red) and CD31 (brown) antigens. These 2 stains were used simultaneously to differentiate between hematopoietic stem and progenitor cells (CD34 1 /CD31 2 ) and vascular endothelium (CD34 1 /CD31 1 ). Distances from hematopoietic CD34 1 cells and blood vessels to the nearest bone trabecula surface were measured digitally and then binned in 50-mm increments, with the results then normalized per unit area of marrow tissue. The distances separating hematopoietic CD34 1 cells from vessels were also tallied. Results: Hematopoietic CD34 1 cells were found to exist along a linear spatial gradient with a maximal areal concentration localized within the first 50 mm of the bone surfaces. An exponential spatial concentration gradient was found in the concentration of blood vessel fragments within the images. Distances between hematopoietic CD34 1 cells and blood vessels exhibited a lognormal distribution indicating a shared spatial niche. Conclusion: Study results confirm that the spatial gradient of hematopoietic stem and progenitor cells previously measured in mouse femur is also present within human cancellous bone. The dosimetric implication of these results may be significant for those scenarios in which the absorbed dose itself is nonuniformly delivered across the marrow tissues, as would be the case for a low-energy b-or a-particle emitter localized on the bone surfaces.

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Section: Discussionmentioning

confidence: 99%

Spatial Distribution of Blood Vessels and CD34+ Hematopoietic Stem and Progenitor Cells Within the Marrow Cavities of Human Cancellous Bone

Watchman¹,

Bourke²,

Lyon³

et al. 2007

Journal of Nuclear Medicine

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show abstract

“…Spiers et al 1978Spiers et al , 1981. The Leeds research group developed a novel optical scanning system from which chord-length distributions were acquired in the lumbar vertebrae for several subjects, as well as at several skeletal sites of a 1.7-y-old child (5 sites), a 9-y-old child (5 sites), and a 44-y-old male (7 sites) Beddoe et al 1976;. These chord-length distributions were used by Whitwell to derive dose factors (marrow and endosteal doses per unit skeletal activity burden) for several radionuclides of interest in radiation protection .…”

Section: Discussionmentioning

confidence: 99%

An Image-Based Computational System for the Design of Radionuclide Therapies for Skeletal Tumors

Bolch¹

2005

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“…The red bone marrow is the doselimiting organ in the majority of targeted radionuclide therapy. As has been previously reviewed (14,15), the anatomic data used to generate the red marrow S values for the adult male of Pamphlet 11 and MIRDOSE3 came from a single 44-y-old man (16,17). Because the marrow mass was not available from this subject, the masses used to convert the absorbed fractions to S values came from studies dating to 1926 (18,19).…”

Section: See Page 645mentioning

confidence: 99%

Toward Patient-Friendly Cell-Level Dosimetry

Sgouros

2007

Journal of Nuclear Medicine

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Measurements of trabecular bone structure in man (for radionuclide dosimetry

Cited by 88 publications

References 12 publications

Spatial Distribution of Blood Vessels and CD34+ Hematopoietic Stem and Progenitor Cells Within the Marrow Cavities of Human Cancellous Bone

Spatial Distribution of Blood Vessels and CD34+ Hematopoietic Stem and Progenitor Cells Within the Marrow Cavities of Human Cancellous Bone

An Image-Based Computational System for the Design of Radionuclide Therapies for Skeletal Tumors

Toward Patient-Friendly Cell-Level Dosimetry

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